The Status of Polychlorinated Biphenyls (PCBs) Extract from Zhanjiang Mangrove Sediments and the Effects on Tissue Structure and Inflammatory Cytokines in Zebrafish Liver

Qamar, Aftab; Waheed, Javaria; Mohyuddin, Sahar Ghulam; Chen, Zhibao; Kang, Dan-Ju; Li, Zhang; Anthony, Glen Hay; Gooneratne, Ravi; Nie, Fang-Hong; Chen, Jinjun

doi:10.1007/s00128-021-03439-6

Cited by 5 publications

(4 citation statements)

References 30 publications

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“…In the treatment group, PCBs were dispersed into the culture medium (Double distilled H 2 O) with a final concentration of 5 μg/L (L-PCBs), 10 μg/L (M-PCBs), and 15 μg/L (H-PCBs). The selection of these exposure concentrations was determined by the environmental-related concentrations (27~35 μg/kg) previously detected in mangrove sediments in our laboratory ( 12 ), as well as the previous literature on the toxicity of multi-PCBs to zebrafish (10 μg/L) in our laboratory ( 13 ).…”

Section: Methodsmentioning

confidence: 99%

Effects of Aroclor 1254 on Intestinal Immunity, Metabolism, and Microflora in Zebrafish

et al. 2022

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Polychlorinated biphenyls (PCBs) are widely distributed environmental toxicants, whose biological toxicity is magnified step by step through the transmission of the food chain. However, there is little research about the effect of PCBs on intestinal epithelial barrier function. In this experiment, the effects of PCB exposure on the intestines of zebrafish were evaluated. Animals were exposed to Aroclor 1254 (5 μg/L, 10 μg/L, 15 μg/L). After 21 days, the changes in histology, enzyme biomarkers, intestinal microorganisms, and metabolomics were detected. The inflammation and oxidative stress in the intestines of zebrafish were observed. Additionally, there were significant changes in intestinal microbiota and tissue metabolism, most of which were associated with oxidative stress, inflammation, and lipid metabolism. The results showed that PCBs exposure resulted in intestinal inflammation and oxidative stress in zebrafish.Moreover, intestinal metabolites and intestinal microflora of zebrafish were also disturbed. This study verified that exposure can lead to intestinal damage and changes in intestinal metabolic capacity and microorganisms, enlightening the consequences of PCB exposure.

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Section: Methodsmentioning

confidence: 99%

Effects of Aroclor 1254 on Intestinal Immunity, Metabolism, and Microflora in Zebrafish

et al. 2022

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“…Chronic inflammation is one of the key features of these metabolic disorders ( 13 ). Data from animal model studies and in vitro studies have reported an association between PCB exposure and inflammation ( 14 – 16 ).…”

Section: Introductionmentioning

confidence: 99%

Anthocyanin-rich blue potato meals protect against polychlorinated biphenyl-mediated disruption of short-chain fatty acid production and gut microbiota profiles in a simulated human digestion model

et al. 2023

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BackgroundPolychlorinated biphenyls (PCBs) are ubiquitous environmental pollutants associated with a wide variety of adverse human health outcomes. PCB 126 and PCB 153 are among the most prevalent congeners associated with human exposure. Emerging studies have suggested that PCB exposure leads to lower gut microbial diversity although their effects on microbial production of health promoting short-chain fatty acids (SCFAs) has been scarcely studied. Blue potatoes are rich in anthocyanins (ACNs), which is a class of polyphenols that promote the growth of beneficial intestinal bacteria such as Bifidobacterium and Lactobacillus and increase the generation of SCFAs. A batch-culture, pH-controlled, stirred system containing human fecal microbial communities was utilized to assess whether human gut microbiota composition and SCFA production are affected by: (a) PCB 126 and PCB 153 exposure; and (b) ACN-rich digests in the presence and absence of the PCB congeners.MethodsAnthocyanin-rich blue potato meals (11.03 g) were digested over 12 h with and without PCB 126 (0.5 mM) and PCB 153 (0.5 mM) using an in vitro simulated gut digestion model involving upper gastrointestinal digestion followed by metabolism by human fecal microbiota. Fecal digests were collected for analysis of gut microbial and SCFA profiles.ResultsPolychlorinated biphenyl-exposed fecal samples showed a significant (p < 0.05) decrease in species richness and a significantly (p < 0.05) different microbial community structure. PCB treatment was associated with an increased (p < 0.05) relative abundance of Akkermansia, Eggerthella, and Bifidobacterium and a decreased (p < 0.05) relative abundance of Veillonella, Streptococcus, and Holdemanella. ACN digests counteracted the altered abundances of Akkermansia and Bifidobacterium seen with the PCB treatment. PCB exposure was associated with a significant (p < 0.05) decrease in total SCFA and acetate concentrations. ACN digests were associated with significantly (p < 0.05) higher SCFA and acetate concentrations in the presence and absence of PCBs.ConclusionHuman fecal matter exposed to PCB 126 and PCB 153 led to decreased abundance and altered gut microbiota profiles as well as lowered SCFA and acetate levels. Importantly, this study showed that prebiotic ACN-rich potatoes counteract PCB-mediated disruptions in human gut microbiota profiles and SCFA production.

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“…Different endogenous or exogenous stimuli can cause intestinal damage and lead to related intestinal diseases 9,10 . It was found that polychlorinated biphenyls (PCBs) extract could induce the overexpression of immune cytokines and inflammatory responses in the intestinal mucosa of zebrafish 11 . Moreover, PCB126 can destroy the intestinal flora of mice and eventually cause host metabolic dysfunction by activating the aromatic hydrocarbon receptors 12 .…”

Section: Introductionmentioning

confidence: 99%

“…9,10 It was found that polychlorinated biphenyls (PCBs) extract could induce the overexpression of immune cytokines and inflammatory responses in the intestinal mucosa of zebrafish. 11 Moreover, PCB126 can destroy the intestinal flora of mice and eventually cause host metabolic dysfunction by activating the aromatic hydrocarbon receptors. 12 Currently, although correlational research have shown the effect of PCB126 on intestinal injury, the underlying molecular mechanism of PCB126 induced colon injury in mice is still unclear.…”

Section: Introductionmentioning

confidence: 99%

Melatonin alleviates 3,3′,4,4′,5‐pentachlorobenzene induced colon injury by relieving oxidative stress

Zhang,

Li,

Xia

et al. 2023

Environmental Toxicology

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Abstract3,3′,4,4′,5‐pentachlorobiphenyl (PCB126) is widely distributed, non‐degradable and bioaccumulative, which can affect the function of tissues and organs of the living organisms. Melatonin (MT) is a sort of indole neurohormone that is mainly secreted by the pineal gland. Numerous studies have shown that MT can alleviate intestinal injury through various mechanisms such as antioxidant, anti‐inflammatory, and anti‐apoptosis. For the above reasons, the aim of this study is to explore the mechanism of intestinal injury in mice after exposure to PCB126 as well as the antagonistic effect of MT. Mice were respectively fed PCB126 (0.326 mg/kg) and/or MT (10 mg/kg) in vivo. In vitro, colonic epithelial cells (MCEC) were treated with PCB126 (150 μM) and/or MT (2 mM). We found that the microscopic structure of colon tissue was impaired after exposure to PCB126. The levels of oxidative stress, the protein and mRNA levels of expression of inflammatory related factors were significantly increased and the expression levels of intestinal tight junction protein were decreased. Notably, MT can promote Nrf2/HO‐1 expression level and reduce the colonic injury caused by PCB126. Further in vitro treatment with reactive oxygen species inhibitors (NAC) showed that it significantly alleviated PCB126‐induced in MCEC cell damage. In summary, the above results suggested that MT alleviates PCB126‐induced colon inflammation by inhibiting the overproduction of reactive oxygen species (ROS) and up‐regulating the expression level of intestinal tight junction protein. Our results contribute to the further comprehension of the intestinal toxicity effects of PCB126 and the significant role of MT in preserving the mechanisms of intestinal injury.

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The Status of Polychlorinated Biphenyls (PCBs) Extract from Zhanjiang Mangrove Sediments and the Effects on Tissue Structure and Inflammatory Cytokines in Zebrafish Liver

Cited by 5 publications

References 30 publications

Effects of Aroclor 1254 on Intestinal Immunity, Metabolism, and Microflora in Zebrafish

Effects of Aroclor 1254 on Intestinal Immunity, Metabolism, and Microflora in Zebrafish

Anthocyanin-rich blue potato meals protect against polychlorinated biphenyl-mediated disruption of short-chain fatty acid production and gut microbiota profiles in a simulated human digestion model

Melatonin alleviates 3,3′,4,4′,5‐pentachlorobenzene induced colon injury by relieving oxidative stress

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