The status of the CRISPR/Cas9 research in plant–nematode interactions

Dutta, Tushar K.; Ray, Soham; Phani, Victor

doi:10.1007/s00425-023-04259-0

Cited by 4 publications

(3 citation statements)

References 113 publications

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“…2022; Hellekes et al . 2023; Dutta et al . 2023); it is only with our work and with recent work from Mengyi Cao (personal communication) that CRISPR-Cas9 has been extended to entomopathogenic nematodes.…”

Section: Discussionmentioning

confidence: 99%

“…The resulting alleles were consistent with a mixture of NHEJ and MMEJ double-strand break repair (Xue and Greene 2021); we saw no evidence of homology-directed repair using the oligonucleotide template we had included. CRISPR-Cas9 is used extensively in established laboratory research animals and is emerging as a powerful tool in a growing variety of nematodes that have not previously been used extensively in laboratory research (Mendez et al 2022;Cadd et al 2022;Hellekes et al 2023;Dutta et al 2023); it is only with our work and with recent work from Mengyi Cao (personal communication) that CRISPR-Cas9 has been extended to entomopathogenic nematodes. We wish to particularly highlight the potential utility of the skpo-2 locus in performing CRISPR studies using other Steinernema entomopathogens.…”

Section: Prospects For Crispr-cas9 Gene Targeting In Steinernemamentioning

confidence: 99%

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Molecular identification of a peroxidase gene controlling body size in the entomopathogenic nematodeSteinernema hermaphroditum

Schwartz,

Tan,

Peraza

et al. 2023

Preprint

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The entomopathogenic nematodeSteinernema hermaphroditumwas recently rediscovered and is being developed as a genetically tractable experimental system for the study of previously unexplored biology, including parasitism of its insect hosts and mutualism with its bacterial endosymbiontXenorhabdus griffiniae. Through whole-genome re-sequencing and genetic mapping we have for the first time molecularly identified the gene responsible for a mutationally defined phenotypic locus in an entomopathogenic nematode. In the process we observed an unexpected mutational spectrum following EMS mutagenesis in this species. We find that the ortholog of the essentialC. elegansperoxidase geneskpo-2controls body size and shape inS. hermaphroditum. We confirmed this identification by inactivating the gene using CRISPR-Cas9. We propose that the identification ofskpo-2will accelerate gene targeting in otherSteinernemaentomopathogenic nematodes used commercially in pest control, asskpo-2is X-linked and males hemizygous for loss of its function can mate, makingskpo-2an easily recognized and maintained marker for use in co-CRISPR.

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Section: Discussionmentioning

confidence: 99%

Section: Prospects For Crispr-cas9 Gene Targeting In Steinernemamentioning

confidence: 99%

Molecular identification of a peroxidase gene controlling body size in the entomopathogenic nematodeSteinernema hermaphroditum

Schwartz,

Tan,

Peraza

et al. 2023

Preprint

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“…Editing GmARF16 and GmMYC2 has potential for enhancing salt tolerance Gene-editing technology has provided scientists with an opportunity to change or introduce single-nucleotide changes in plant genomes with high precision and without the need for double-strand breaks (Molla et al, 2021;Wang & Doudna, 2023). Base editing is a promising tool for plant breeding because it can be used to introduce bespoke edits that can, or already has, significantly enhanced yields, improve nutritional value or confer stress resistance (Hua et al, 2018;Eraslan et al, 2019;Dutta et al, 2023;Subburaj & Agapito-Tenfen, 2023). For instance, a point mutation in the miR156 binding site of OsSPL14 leads to rice plants with improved architecture and higher seed yields (Jiao et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

A novel miR160a–GmARF16–GmMYC2 module determines soybean salt tolerance and adaptation

Wang,

Li,

Zhuang

et al. 2023

New Phytologist

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Summary Salt stress is a major challenge that has a negative impact on soybean growth and productivity. Therefore, it is important to understand the regulatory mechanism of salt response to ensure soybean yield under such conditions. In this study, we identified and characterized a miR160a–GmARF16–GmMYC2 module and its regulation during the salt–stress response in soybean. miR160a promotes salt tolerance by cleaving GmARF16 transcripts, members of the Auxin Response Factor (ARF) family, which negatively regulates salt tolerance. In turn, GmARF16 activates GmMYC2, encoding a bHLH transcription factor that reduces salinity tolerance by down‐regulating proline biosynthesis. Genomic analysis among wild and cultivated soybean accessions identified four distinct GmARF16 haplotypes. Among them, the GmARF16H3 haplotype is preferentially enriched in localities with relatively saline soils, suggesting GmARF16H3 was artificially selected to improve salt tolerance. Our findings therefore provide insights into the molecular mechanisms underlying salt response in soybean and provide valuable genetic targets for the molecular breeding of salt tolerance.

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Molecular identification of a peroxidase gene controlling body size in the entomopathogenic nematode Steinernema hermaphroditum

Schwartz,

Tan,

Peraza

et al. 2023

Genetics

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The entomopathogenic nematode Steinernema hermaphroditum was recently rediscovered and is being developed as a genetically tractable experimental system for the study of previously unexplored biology, including parasitism of its insect hosts and mutualism with its bacterial endosymbiont Xenorhabdus griffiniae. Through whole-genome re-sequencing and genetic mapping we have for the first time molecularly identified the gene responsible for a mutationally defined phenotypic locus in an entomopathogenic nematode. In the process we observed an unexpected mutational spectrum following EMS mutagenesis in this species. We find that the ortholog of the essential C. elegans peroxidase gene skpo-2 controls body size and shape in S. hermaphroditum. We confirmed this identification by generating additional loss-of-function mutations in the gene using CRISPR-Cas9. We propose that the identification of skpo-2 will accelerate gene targeting in other Steinernema entomopathogenic nematodes used commercially in pest control, as skpo-2 is X-linked and males hemizygous for loss of its function can mate, making skpo-2 an easily recognized and maintained marker for use in co-CRISPR.

show abstract

The status of the CRISPR/Cas9 research in plant–nematode interactions

Cited by 4 publications

References 113 publications

Molecular identification of a peroxidase gene controlling body size in the entomopathogenic nematodeSteinernema hermaphroditum

Molecular identification of a peroxidase gene controlling body size in the entomopathogenic nematodeSteinernema hermaphroditum

A novel miR160a–GmARF16–GmMYC2 module determines soybean salt tolerance and adaptation

Molecular identification of a peroxidase gene controlling body size in the entomopathogenic nematode Steinernema hermaphroditum

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