“…Concentration of ergosterol in a fungus is influenced by both internal and external factors, such as fungal species (Huang et al 1985, Newell et al 1987, Salmanowicz and Nylund 1988, physiological state of fungus, its age, developmental stage (Huang et al 1985), general growth conditions, chemical composition of substrate, temperature and pH of the environment (Weete 1980, Arnezeder and Hampel 1991, Olsen 1973, Vanden Bossche 1990. Therefore, the condi- tions of fungus should closely mimic that of the anticipated environment (Gessner and Chauvet 1993).…”