The stimulating influence of the raph� nuclei on the morphofunctional development of the hippocampus during their combined cultivation

Abstract

It has been demonstrated in combined (raphé nuclei-hippocampus, cerebellum-hippocampus) organotypical cultures of the brain of newborn rats that, unlike cerebellar explants, the raphé nuclei grow in an oriented manner toward the hippocampus and exert a stimulating influence on its morphofunctional development; this is manifested: a) in an intense proliferation and migration of glial cells, which are accompanied by accelerated growth of neuronal processes and an increase in the dimensions of the initial piece; b) in the earlier formation of the background electrical activity of the neurons; c) in an increase in these cultures in the number of active cells; d) in a significant predominance of neurons with periodic (in the form of trains or groups) discharges. These data are in agreement with previously established effects of exogenous serotonin, and attest to an important role of the serotoninergic influences of the raphé nuclei in the morphofunctional development of the hippocampus.

Antiplatelet activity of 3-butyl-6-bromo-1(3H)-isobenzofuranone on rat platelet aggregation

“…Besides, PF 4 is released in combination at the site of injury or endothelial inflammation, even affect greater than described experimentally likely to occur in vivo [37]. 5-HT and PF 4 are widely recognized as activation symbols in lots of research [38,39]. Serotonin exists in hippocampus, frontal neocortex and platelets, being stored in subcellular storage vesicles at the terminal branches of neurons.…”

Juvenile stress attenuates the dorsal hippocampal postsynaptic 5-HT1A receptor function in adult rats

“…More study is therefore needed to elucidate this issue. Chubakov et al (1993) demonstrated that the serotoninergic influences of the raphe nuclei were important for the morphofunctional development of the hippocampus.…”

Direct and indirect cellular effects of aspartame on the brain

“…Serotonin (5-HT), like other monoamine neurotransmitters, has been shown to play a role in regulating brain development before the time it assumes its role as a neurotransmitter in the mature brain (Chubakov et al, 1986(Chubakov et al, , 1993Lauder, 1990;Whitaker-Azmitia, 1991;Turlejski, 1996;Whitaker-Azmitia et al, 1996). This neurotransmitter is concentrated in the raphe nucleus of the brain, and is also present in platelets.…”