The stimulation of healing within a rat calvarial defect by mPCL–TCP/collagen scaffolds loaded with rhBMP-2

Sawyer, Andrew J.; Song, Santai; Susanto, Evelyn; Chuan, Peiying; Lam, Christopher; Woodruff, Maria A.; Hutmacher, Dietmar W.; Cool, Simon M.

doi:10.1016/j.biomaterials.2008.12.055

Cited by 191 publications

(171 citation statements)

References 30 publications

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“…Besides that, the Fmax values obtained with the titanium scaffolds in our study were close to the ones required for fracturing intact calvarial bones of 12-week-old rats, which were found to range from 80N to 100N 25 .…”

Section: Discussionsupporting

confidence: 79%

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Paired evaluation of calvarial reconstruction with prototyped titanium implants with and without ceramic coating

et al. 2014

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PURPOSE:To investigate the osseointegration properties of prototyped implants with tridimensionally interconnected pores made of the Ti 6 Al 4 V alloy and the influence of a thin calcium phosphate coating. METHODS:Bilateral critical size calvarial defects were created in thirty Wistar rats and filled with coated and uncoated implants in a randomized fashion. The animals were kept for 15, 45 and 90 days. Implant mechanical integration was evaluated with a push-out test.Bone-implant interface was analyzed using scanning electron microscopy. RESULTS:The maximum force to produce initial displacement of the implants increased during the study period, reaching values around 100N for both types of implants. Intimate contact between bone and implant was present, with progressive bone growth into the pores. No significant differences were seen between coated and uncoated implants. CONCLUSION:Adequate osseointegration can be achieved in calvarial reconstructions using prototyped Ti 6 Al 4 V Implants with the described characteristics of surface and porosity.

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Section: Discussionsupporting

confidence: 79%

“…In an evaluation of the influence of growth hormone supplementation on calvarial healing using an identical protein-2 (BMP-2) after 15 weeks 25 , and of 138N using calcium deficient hydroxyapatite (CDHA) implants coated with chitosan and treated with BMP-2 after eight weeks 26 .…”

Section: Discussionmentioning

confidence: 99%

Paired evaluation of calvarial reconstruction with prototyped titanium implants with and without ceramic coating

et al. 2014

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“…Moreover the application of BMP in irradiated rat mandibles resulted in predictable bone generation (Springer et al, 2008). The release of rhBMP-2 from collagen scaffolds is a clinically applicable approach to repair and regenerate critically sized craniofacial bone defects in a rat model (Sawyer et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

Influence of rhBMP-2 on bone formation and osseointegration in different implant systems after sinus-floor elevation. An in vivo study on sheep

Gutwald

Haberstroh

Stricker

et al. 2010

Journal of Cranio-Maxillofacial Surgery

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SUMMARY.Background: Several studies have reported certain bone morphogenic proteins (BMPs) to have positive effects on bone generation. Although some investigators have studied the effects of human recombinant BMP (rhBMP-2) in sinus augmentation in sheep, none of these studies looked at the placement of implants at the time of sinus augmentation. Furthermore, no literature could be found to report on the impact that different implant systems, as well as the positioning of the implants had on bone formation if rhBMP-2 was utilized in sinus-lift procedures. Purpose: The aim of this study was to compare sinus augmentation with rhBMP-2 on a poly-D, L-lactic-co-glycolic acid gelatine (PLPG) sponge with sinus augmentation with autologous pelvic cancellous bone in the maxillary sinus during the placement of different dental implants. Materials and methods: Nine adult female sheep were submitted to bilateral sinus-floor elevation. In one side (test group) the sinus lift was performed with rhBMP-2 on a PLPG-sponge, while the contralateral side served as the control by using cancellous bone from the iliac crest. Three different implants (Bränemark Ò , 3i Ò and Straumann Ò ) were inserted either simultaneously with the sinus augmentation or as a two staged procedure 6 weeks later. The animals were sacrificed at 6 and 12 weeks for histological and histomorphometrical evaluations during which boneto-implant contact (BIC) and bone density (BD) were evaluated. Results: BD and BIC were significantly higher at 12 weeks in the test group if the implants were placed at the time of the sinus lift (p \ 0.05). No difference was observed between the different implant systems or positions. Conclusions: The use of rhBMP-2 with PLPG-sponge increased BIC as well as BD in the augmented sinuses if compared to autologous bone. Different implant systems and positions of the implants had no effect on BIC or BD. Ó 2010 European Association for Cranio-Maxillo-Facial Surgery

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“…These osteoconductive scaffolds are often combined with growth factors (osteoinductive) (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) and/or with cells (osteogenic) (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32) to induce active stimulation of bone regeneration. The objective of most of these investigations is to enable bone defect healing, which remains an unsolved clinical problem.…”

mentioning

confidence: 99%

Porous scaffold architecture guides tissue formation

Cipitria

Lange

Schell

et al. 2012

Journal of Bone and Mineral Research

104

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Critical-sized bone defect regeneration is a remaining clinical concern. Numerous scaffold-based strategies are currently being investigated to enable in vivo bone defect healing. However, a deeper understanding of how a scaffold influences the tissue formation process and how this compares to endogenous bone formation or to regular fracture healing is missing. It is hypothesized that the porous scaffold architecture can serve as a guiding substrate to enable the formation of a structured fibrous network as a prerequirement for later bone formation. An ovine, tibial, 30-mm critical-sized defect is used as a model system to better understand the effect of the scaffold architecture on cell organization, fibrous tissue, and mineralized tissue formation mechanisms in vivo. Tissue regeneration patterns within two geometrically distinct macroscopic regions of a specific scaffold design, the scaffold wall and the endosteal cavity, are compared with tissue formation in an empty defect (negative control) and with cortical bone (positive control). Histology, backscattered electron imaging, scanning small-angle X-ray scattering, and nanoindentation are used to assess the morphology of fibrous and mineralized tissue, to measure the average mineral particle thickness and the degree of alignment, and to map the local elastic indentation modulus. The scaffold proves to function as a guiding substrate to the tissue formation process. It enables the arrangement of a structured fibrous tissue across the entire defect, which acts as a secondary supporting network for cells. Mineralization can then initiate along the fibrous network, resulting in bone ingrowth into a critical-sized defect, although not in complete bridging of the defect. The fibrous network morphology, which in turn is guided by the scaffold architecture, influences the microstructure of the newly formed bone. These results allow a deeper understanding of the mode of mineral tissue formation and the way this is influenced by the scaffold architecture. ß

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The stimulation of healing within a rat calvarial defect by mPCL–TCP/collagen scaffolds loaded with rhBMP-2

Cited by 191 publications

References 30 publications

Paired evaluation of calvarial reconstruction with prototyped titanium implants with and without ceramic coating

Paired evaluation of calvarial reconstruction with prototyped titanium implants with and without ceramic coating

Influence of rhBMP-2 on bone formation and osseointegration in different implant systems after sinus-floor elevation. An in vivo study on sheep

Porous scaffold architecture guides tissue formation

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