The Stop Signal Controls the Efficiency of Release Factor-Mediated Translational Termination

Tate, Warren P.; Dalphin, Mark E.; Pel, Herman J.; Mannering, Sally A.

doi:10.1007/978-1-4899-1766-9_10

Cited by 6 publications

(1 citation statement)

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“…The Class I polypeptide chain release factors (RFs) are proteins involved in decoding the translational termination signal (1). A tRNA analogue model for how RFs function was proposed after evidence that these proteins spanned the decoding site of the small subunit of the ribosome and the peptidyltransferase centre of the large subunit like a tRNA (2).…”

Section: Introductionmentioning

confidence: 99%

Translational termination in Escherichia coli: three bases following the stop codon crosslink to release factor 2 and affect the decoding efficiency of UGA-containing signals

Poole

Major

Mannering

et al. 1998

Nucleic Acids Research

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The observations that the Escherichia coli release factor 2 (RF2) crosslinks with the base following the stop codon (+4 N), and that the identity of this base strongly influences the decoding efficiency of stop signals, stimulated us to determine whether there was a more extended termination signal for RF2 recognition. Analysis of the 3' contexts of the 1248 genes in the E.coli genome terminating with UGA showed a strong bias for U in the +4 position and a general bias for A and against C in most positions to +10, consistent with the concept of an extended sequence element. Site-directed crosslinking occurred to RF2 from a thio-U sited at the +4, +5 and +6 bases following the UGA stop codon but not beyond (+7 to +10). Varying the +4 to +6 bases modulated the strength of the crosslink from the +1 invariant U to RF2. A strong selection bias for particular bases in the +4 to +6 positions of certain E. coli UGANNN termination sites correlated in some cases with crosslinking efficiency to RF2 and in vivo termination signal strength. These data suggest that RF2 may recognise at least a hexanucleotide UGA-containing sequence and that particular base combinations within this sequence influence termination signal decoding efficiency.

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Section: Introductionmentioning

confidence: 99%