The storage of nutritional resources during vitellogenesis of Panstrongylus megistus (Hemiptera: Reduviidae): The pathways of lipophorin in lipid delivery to developing oocytes

Fruttero, Leonardo L.; Frede, Silvia; Rubiolo, Edilberto R.; Canavoso, Lilián Etelvina

doi:10.1016/j.jinsphys.2011.01.009

Cited by 35 publications

(34 citation statements)

References 50 publications

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“…As was previously reported in D. maxima (Aguirre et al, 2008) as well as in other species (Kotaki, 2003;Fruttero et al, 2011), the high levels of hemolymphatic vitellogenin after a blood meal were consistent with the pronounced remodeling of ovarioles observed during vitellogenesis, and with the increasing amounts of vitellin found at the ovarian tissue. However, two remarkable findings need a special consideration.…”

Section: Discussionsupporting

confidence: 89%

“…As expected, vitellogenic ovaries of D. maxima were able to perform active uptake and deposition of yolk proteins (Supplementary video). A remarkable level of vitellogenin endocytosis during vitellogenesis was also described in R. prolixus (Oliveira et al, 1986) and more recently, in Panstrogylus megistus (Fruttero et al, 2011). In contrast, uptake of vitellogenin by terminal oocytes was minimal at early follicular atresia (Fig.…”

Section: Discussionmentioning

confidence: 54%

“…The homogenates were first centrifuged at 2500 Â g for 10 min at 4 C and the floating fat cake and pellet were discarded. The resulting material was centrifuged at 15,000 Â g for 30 min at 4 C and the supernatants collected and used for ELISA as previously reported (Fruttero et al, 2011). Protein concentration was determined according to Bradford (1976), using bovine serum albumin as standard.…”

Section: Vitellogenin and Vitellin Quantificationmentioning

confidence: 99%

“…In vivo uptake assays were assessed by using purified vitellogenin from hemolymph of vitellogenic females obtained by a combination of KBr gradient ultracentrifugation followed by ionexchange chromatography on DEAE-Trisacryl-M (Fruttero et al, 2011). Purified vitellogenin was labeled with FITC (Vg-FITC, 1 mg/ml) in DMSO as described elsewhere (Hermanson, 1996).…”

Section: In Vivo Vitellogenin Uptake By Oocytesmentioning

confidence: 99%

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Biochemical changes in the transition from vitellogenesis to follicular atresia in the hematophagous Dipetalogaster maxima (Hemiptera: Reduviidae)

Aguirre

Fruttero

Leyria

et al. 2011

Insect Biochemistry and Molecular Biology

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 54%

Section: Vitellogenin and Vitellin Quantificationmentioning

confidence: 99%

Section: In Vivo Vitellogenin Uptake By Oocytesmentioning

confidence: 99%

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Biochemical changes in the transition from vitellogenesis to follicular atresia in the hematophagous Dipetalogaster maxima (Hemiptera: Reduviidae)

Aguirre

Fruttero

Leyria

et al. 2011

Insect Biochemistry and Molecular Biology

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“…Adult insect females naturally produce a great amount of eggs to transmission their genesis and to that end they participate significant resources [11]. In the period of oogenesis, carbohydrates, lipids and proteins other substances are commonly accumulated in the development oocyte [12]. Synthesis of yolk proteins is a vital process for the growth of the egg.…”

Section: Introductionmentioning

confidence: 99%

Ovarian Development of House Fly (Musca domestica L.) (Diptera: Muscidae)

Xider

Amin

2018

KJAR

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Abstract:The ovarian development of Musca domestica, was examined under light microscope. There are two ovaries in the adult female of fly. Each ovary was formed of about 60-70 polytrophic ovarioles. The ovariole in cross sections organized in several circles. From cytological observations, ovary development and oogenesis could be divided in to 6 stages; ovaries with no noticeable follicle cells existing were characterized as (stage1), the follicle-growing stages considered as (stage2, 3), the vitellogenen in stages (stages4, 5(and mature egg stage (stage6). The oocyte initiated development at first stages of oogenesis; the nurse cells and follicle epithelial cells were considered to fulfill important functions with regard to the growth of the oocyte.

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OVARIAN NUTRITIONAL RESOURCES DURING THE REPRODUCTIVE CYCLE OF THE HEMATOPHAGOUSDIPETALOGASTER MAXIMA(HEMIPTERA: REDUVIIDAE): FOCUS ON LIPID METABOLISM

Leyria

Fruttero

Aguirre

et al. 2014

Arch Insect Biochem Physiol

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In this study, we have analyzed the changes of the ovarian nutritional resources in Dipetalogaster maxima at representative days of the reproductive cycle: previtellogenesis, vitellogenesis, as well as fasting-induced early and late atresia. As expected, the amounts of ovarian lipids, proteins, and glycogen increased significantly from previtellogenesis to vitellogenesis and then, diminished during atresia. However, lipids and protein stores found at the atretic stages were higher in comparison to those registered at previtellogenesis. Specific lipid staining of ovarian tissue sections evidenced remarkable changes in the shape, size, and distribution of lipid droplets throughout the reproductive cycle. The role of lipophorin (Lp) as a yolk protein precursor was analyzed by co-injecting Lp-OG (where OG is Oregon Green) and Lp-DiI (where DiI is 1,10-dioctadecyl-3,3,30,30-tetramethylindocarbocyanine) to follow the entire particle, demonstrating that both probes colocalized mainly in the yolk bodies of vitellogenic oocytes. Immunofluorescence assays also showed that Lp was associated to yolk bodies, supporting its endocytic pathway during vitellogenesis. The involvement of Lp in lipid delivery to oocytes was investigated in vivo by co-injecting fluorescent probes to follow the fate of the entire particle (Lp-DiI) and its lipid cargo (Lp-Bodipy-FA). Lp-DiI was readily incorporated by vitellogenic oocytes and no lipoprotein uptake was observed in terminal follicles of ovaries at atretic stages. Bodipy-FA was promptly transferred to vitellogenic oocytes and, to a much lesser extent, to previtellogenic follicles and to oocytes of ovarian tissue at atretic stages. Colocalization of Lp-DiI and Lp-Bodipy-FA inside yolk bodies indicated the relevance of Lp in the buildup of lipid and protein oocyte stores during vitellogenesis.

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The storage of nutritional resources during vitellogenesis of Panstrongylus megistus (Hemiptera: Reduviidae): The pathways of lipophorin in lipid delivery to developing oocytes

Cited by 35 publications

References 50 publications

Biochemical changes in the transition from vitellogenesis to follicular atresia in the hematophagous Dipetalogaster maxima (Hemiptera: Reduviidae)

Biochemical changes in the transition from vitellogenesis to follicular atresia in the hematophagous Dipetalogaster maxima (Hemiptera: Reduviidae)

Ovarian Development of House Fly (Musca domestica L.) (Diptera: Muscidae)

OVARIAN NUTRITIONAL RESOURCES DURING THE REPRODUCTIVE CYCLE OF THE HEMATOPHAGOUSDIPETALOGASTER MAXIMA(HEMIPTERA: REDUVIIDAE): FOCUS ON LIPID METABOLISM

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