The strategies to DVI challenges in Typhoon Morakot

Lin, Chun-Yen; Huang, Tsun-Ying; Shih, Hsuan-Cheng; Yuan, Chiao-Hsuan; Chen, Liang-Ju; Tsai, Hua-Sheng; Pan, Chih-Hsin; Chiang, Hui-Ming; Liu, Hsin-Lin; Su, Wu‐Chung; Wu, Kun-Tien; Chao, Chi-Hsiang; Huang, Nu-En; Yang, Kun-Da; Hsieh, Hsing-Mei; Tsai, Li-Chin; Linacre, Adrian; Yu, Yu-Jen; Lin, Yen-Yang; Chu, Pao-Ching; Lee, James Chun-I

doi:10.1007/s00414-010-0479-8

Cited by 16 publications

(10 citation statements)

References 17 publications

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“…The selection of the bone element for molecular analysis is an important step, as DNA is not preserved equally in skeletal elements from different anatomical regions of the human body [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ]. The bone element type that offers the highest DNA yield, especially in ancient skeletons, is the temporal bone, in particular the inner ear of the petrous bone [ 29 , 30 , 31 , 32 , 33 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…In such cases, other bones have to be selected for the genetic testing of aged skeletons; long bones (mainly femur) are preferred for analyses [ 4 ], while metacarpal and other short bone elements provide promising or even better results [ 36 ]. Therefore, several bones from the same skeleton should be collected whenever possible [ 4 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ]. Regardless of the type of bone selected, the intrabone part plays an important role because of the high variability of DNA preservation observed not only between different bones, but also within an individual bone.…”

Section: Discussionmentioning

confidence: 99%

“…Although high-throughput shotgun sequencing and the analysis of genome-wide data have largely replaced current PCR-based methods in ancient DNA (aDNA) analysis [ 1 , 2 , 13 ], there are many aspects that aDNA analysis and forensic DNA analysis have in common; for instance, the use of limited amounts of degraded DNA, the precautions adopted to prevent contamination, and the use of authenticity criteria [ 14 , 15 , 16 , 17 , 18 , 19 , 20 ]. In addition, both disciplines have developed strategies to select the skeletal element which provides, a priori, the highest probabilities of positive outcomes [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

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The Baron Pasquale Revoltella’s Will in the Forensic Genetics Era

Fattorini

Previderé

Bonin

et al. 2023

Genes

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In this article, we describe multiple analytical strategies that were first developed for forensic purposes, on a set of three bone samples collected in 2011. We analyzed a single bone sample (patella) collected from the artificially mummified body of the Baron Pasquale Revoltella (1795–1869), as well two femurs which allegedly belonged to the Baron’s mother (Domenica Privato Revoltella, 1775–1830). Likely due to the artificial mummification procedures, the inner part of the Baron’s patella allowed the extraction of high-quality DNA yields, which were successfully used for PCR-CE and PCR-MPS typing of autosomal, Y-specific, and mitochondrial markers. The samples extracted from the trabecular inner part of the two femurs yielded no typing results by using the SNP identity panel, whereas the samples extracted from the compact cortical part of the same bone samples allowed genetic typing, even by the employment of PCR-CE technology. Altogether, 10/15 STR markers, 80/90 identity SNP markers, and HVR1, HVR2, and HVR3 regions of the mtDNA were successfully typed from the Baron’s mother’s remains by the combined use of PCR-CE and PCR-MPS technologies. The kinship analysis showed a likelihood ratio of at least 9.1 × 106 (corresponding to a probability of maternity of 99.9999999%), and thus confirmed the identity of the skeletal remains as those of the Baron’s mother. This casework represented a challenging trial for testing forensic protocols on aged bones samples. It highlighted the importance of accurately sampling from the long bones, and that DNA degradation is not blocked by freezing at −80 °C.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Baron Pasquale Revoltella’s Will in the Forensic Genetics Era

Fattorini

Previderé

Bonin

et al. 2023

Genes

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“…Seven victims were identified as grandparents or grandchildren based on their probable age. 19 Skinner et al 2010, in their article mentioned that in between 1991-1999, in Yugoslavia conflict, out of 30000 missing individuals, two-third of the 15000 victims were identified through DNA. 23% of 3919 remains were identified through dental information.…”

Section: Dna Analysis In Mass Disastersmentioning

confidence: 99%

DNA analysis in identifying mass disaster victims

Shanbhag¹,

Vaswani²,

Pramod³

2020

IJFMTS

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Disaster victim identification is very important for legal, administration, and humanity reasons. Disasters include airplane crashes, fire disasters, volcanoes, hurricane, tornadoes, wars and flood. Methods of identification include visual identification, fingerprints, DNA analysis, personal objects and odontology. DNA analysis is an important method of disaster victim identification particularly when other methods of identification are not possible or not conclusive. The current article enumerates and reviews the role played by DNA analysis in identifying mass disaster victims.

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“…If there are direct genetic relatives, such as an offspring and both genetic parents, then this process is well established; however if there are only putative siblings then the opportunity for misidentification increases. Such instances arose in the identification of deceased from a mass disaster (3). The comparison of the DNA profiles of two putative siblings can result in a likelihood ratio (LR) or combined sibling index (SI), being the odds favoring the tested samples originating from siblings compared to the samples originating from two genetically unrelated individuals.…”

mentioning

confidence: 99%

A novel strategy for sibship determination in trio sibling model

Lee¹,

Lin²,

Tsai³

et al. 2012

Croat Med J

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AimTo use a virtually simulated population, generated from published allele frequencies based on 15 short tandem repeats (STR), to evaluate the efficacy of trio sibship testing and sibling assignment for forensic purposes.MethodsVirtual populations were generated using 15 STR loci to create a large number of related and unrelated genotypes (10 000 trio combinations). Using these virtual populations, the probability of related and unrelated profiles can be compared to determine the chance of inclusions of being siblings if they are true siblings and the chance of inclusion if they are unrelated. Two specific relationships were tested – two reference siblings were compared to a third true sibling (3S trio, sibling trio) and two reference siblings were compared to an unrelated individual (2S1U trio, non-sibling trio).ResultsWhen the likelihood ratio was greater than 1, 99.87% of siblings in the 3S trio population were considered as siblings (sensitivity); 99.88% of non-siblings in the 2S1U trio population were considered as non-siblings (specificity); 99.9% of both populations were identified correctly as siblings and non-siblings; and the accuracy of the test was 99.88%.ConclusionsThe high sensitivity and specificity figures when using two known siblings compared to a putative sibling are significantly greater than when using only one known relative. The data also support the use of increasing number of loci allowing for greater confidence in genetic identification. The system established in this study could be used as the model for evaluating and simulating the cases with multiple relatives.

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The strategies to DVI challenges in Typhoon Morakot

Cited by 16 publications

References 17 publications

The Baron Pasquale Revoltella’s Will in the Forensic Genetics Era

The Baron Pasquale Revoltella’s Will in the Forensic Genetics Era

DNA analysis in identifying mass disaster victims

A novel strategy for sibship determination in trio sibling model

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