The stroma of the thymus of the rat: Morphology and antigen diffusion, a reconsideration

Abstract

This work reconsiders aspects of the morphology of the capsule, of the blood vasculature, of the distribution of reticular fibers, and of the diffusion of intramediastinally injected antigens in the stroma of the thymus of the rat. This was done by an analysis of standard sections of normal thymuses, of sections of thymuses perfused with colloidal carbon, of silver-impregnated sections, and of sections of thymuses of rats injected intramediastinally with a fluorescent antigen or intravenously with Trypan blue, and by electron microscopy of the thymic capsule. The capsule consisted of two layers: an outer layer covering the entire periphery of a thymic lobe, and an inner layer which outlined the entire convoluted peripheral cortex of a lobe. Cortical vessels entered the capsule and septa in which they formed a capillary network. These capsular capillaries were fenestrated and leukocytes were often present near them. Adipocytes were also seen near these vessels in some areas of the capsule, and often at the bases of septa and trabeculae. Furthermore, much of the medulla had a dense network of coarse reticular fibers, whereas the remainder of the medulla and the cortex contained a loose network of fine fibers stretching out from the capsule, septa, and trabeculae. Intramediastinally injected fluorescent antigens were observed to spread in the capsule and septa and to diffuse in the fiber networks stretched across the cortex and the medulla. Fluorescence also highlighted cortical reticular cells but not the thymocytes. Intravenously injected Trypan blue stained the capsule, the septa, the cortical reticular cells, and the autofluorescent cells outlining the corticomedullary junction of each lobule. The unusual penetration of capillaries from the thymic parenchyma into the thymic capsule suggested that the capsular capillaries participate in peculiar thymic events, such as the recruitment of blood stem-cells. It is concluded that small amounts of blood antigens normally exude from capsular capillaries and diffuse into the fibers extending from the capsule across the cortex. The phenomenon would be increased under conditions causing thymic involution. An explanation is proposed to account for the development of involution which involves the exudation of antigens from the capsular capillaries. A comparable mechanism could also account for the development of a particular experimental immune tolerance.

The vasculature of the guinea-pig thymus: Topographic studies by light and electron microscopy.

“…On the other hand, OLSON and POSTE (1973) described a dual circulation in the guinea pig and the pig thymus, where some capillaries drain into subcapsular veins while others loop back to join venules at the cortico-medullary junction. Such a pattern was also seen by SAINTE-MARIE et al (1986) in the rat. KARDON and KESSEL (1981), examining vascular resin casts of rat thymus by SEM, found that small vessels (20-30,um in diameter) penetrated perpendicularly to the capsular surface and drained the characteristic radial capillaries of the cortex.…”

“…3) A dual circulation: Venous blood of the cortex drains towards the lobular surface as well as towards the cortico-medullary junction. Such a pattern has been reported in the rat (SAINTS-MARIE and MESSIER, 1970;SAINTE-MARIE et al, 1986), guinea pig and pig (OLSON and POSTE, 1973). These studies were all based on light microscope observations of whole mounts or serial sections of the thymus.…”

Ontogeny of IgM‐producing cells in the lymphoid organs of rainbow trout, Salmo gairdneri Richardson: an immuno‐ and enzyme‐histochemical study

“…In fact, cells staining for IgM did not occur in the thymus until the development of the thymic trabeculae. Furthermore, the peculiar location of IgM+ cells at the border between the inner and outer zones of the developing thymus, where trabeculae ramify into several branches, resembles the plasma cell location in the rat thymus, where this fact has been related to an exudation of small amounts of blood-borne antigens from the thymic septa (Sainte-Marie et al, 1986). Monocyte/macrophages play important roles in B-cell differentiation and function in mammals (Gisler et al, 1984,19873).…”

Microvasculature of Normal and Involuted Mouse Thymus

“…1987] and in the rat thymus [Sainte-Marie and Messier. 1970;Sainte-Marie et al, 1986] where corti cal capillaries have a dual drainage pattern, i.e. they con nect either with subcapsular or with medullary veins.…”