The Stromal Vascular Fraction Improves Maintenance of the Fat Graft Volume

Cai, Wei; Yu, L; Tang, Xiaowei; Shen, Gan

doi:10.1097/sap.0000000000001589

Cited by 27 publications

(21 citation statements)

References 32 publications

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“…Therefore, the key to fat regeneration is to increase the content of SVF cells and ASCs. At present, the cell-assisted fat transplantation technology Lipotransfer involves the addition of regenerated cells (ASCs or SVF cells), which has proven to significantly improve the retention rate of fat grafts in small-scale fat transplantation (Zhang et al, 2016;Zhou et al, 2016;Gontijode-Amorim et al, 2017;Li et al, 2017;Cai et al, 2018;Bashir et al, 2019;Hong et al, 2019;Kim et al, 2019). However, this technology is limited based on safety challenges such as enzyme digestion, contamination, and possible promotion of tumor recurrence in the process of regeneration related to the steps of cell separation; these have hindered its clinical use (Fraser et al, 2011;Krumboeck et al, 2013;Orbay et al, 2018;Gebremeskel et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and Cellular Characteristics of a Stromal Vascular Fraction/Extracellular Matrix Gel Prepared Using Mechanical Shear Force on Human Fat

Yuan

Zou

Tan

et al. 2021

Front. Bioeng. Biotechnol.

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The retention of fat-derived grafts remains a challenge for regenerative medicine. Fat aspirates from patients undergoing liposuction were prepared into standard Coleman fat grafts or further isolated using mechanical shear force to prepare a stromal vascular fraction (SVF)/extracellular matrix (ECM) gel. The retention rate of the SVF/ECM gel was significantly higher than that of the Coleman fat at 3, 14, 28, and 60 days following transplantation on the backs of nude mice. The viscosity of the fat was directly proportional to the shearing force. Although the mechanical isolation did not affect the total number of cells, it significantly decreased the number of living cells. Flow cytometry showed a greater number of mesenchymal stem cells, supra-adventitial (SA)-adipose stromal cells (ASCs), and adipose-derived stem cells but a lower number of endothelial progenitor cells in the SVF/ECM gel than in the Coleman fat. Thus, mechanical isolation of fat can increase the pluripotency of adipocytes, which can improve graft retention in cell therapy.

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Section: Discussionmentioning

confidence: 99%

Phenotypic and Cellular Characteristics of a Stromal Vascular Fraction/Extracellular Matrix Gel Prepared Using Mechanical Shear Force on Human Fat

Yuan

Zou

Tan

et al. 2021

Front. Bioeng. Biotechnol.

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“…Дослідження стосувались використання СВФ задля покращення якості шкіри, лікування ран та шрамів, колінного остеоартриту, ахілесової тендинопатії, розладів скроневонижньощелепного суглоба, перианального свища, спричиненого хворобою Крона чи локальною радіотерапією, рубців голосових зв'язок, васкулопатії, андрогенної алопеції, мігрені, діабету, розсіяного склерозу, опіків, проблем із центральною нервовою системою, створення імплантів кісток та полегшення проблем із центральною нервовою системою і опорно-руховим апаратом [11,12,13].…”

Section: іі розвиток застосування свф як джерела стовбурових клітин для регенеративної медициниunclassified

Стромально-Васкулярна Фракція Жирової Тканини: Перспективи Для Регенеративної Біоінженерії

Лисичина¹,

Мотроненко²

2021

БіоІнжТех

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Реферат – Бурхливий розвиток пластичної естетичної хірургії сприяв збільшенню досліджень якостей жирової тканини, яка залишалась після операцій ліпосакції. Це допомогло детально описати її потенціал і з’ясовати, що в стромально-васкулярній фракції (СВФ) жирової тканини містяться стовбурові клітини, які можуть бути потенційним джерелом матеріалів для регенеративної медицини. Наразі ведуться десятки досліджень, спрямованих на вивчення потенціалу СВФ, зокрема її застосування при проблемах шкіри, шрамах, остеоартрозі, тендинопатії, загоєнні ран, андрогенній алопеції, васкулопатії, мігрені та рубцюванні голосових складок. Та попри значну кількість досліджень і можливих перспектив, поки ще немає офіційно затверджених протоколів і дозволів використання цієї методики для лікування конкретних хвороб. Оскільки проведених експериментів ще недостатньо, а правове регулювання цієї сфери не сформовано. У цій роботі аналізуються останні систематичні та стислі літературні огляди, що стосуються біологічних властивостей, клінічних застосувань та регуляторних вимог до СВФ з метою формування загального уявлення про поточний статус СВФ жирової тканини, як альтернативного джерела для регенеративної медицини.

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“…In addition to that, the lipoaspirate can be used in combination with other biological products. These include cell assisted lipo lling (CAL), where fat is combined with adipose-derived stem cells (ADSC) which have been reported to improve fat survival rate (4), ADSC transfer only, fat plus stromal vascular fraction (SVF) transfer, which contains ADSC's, endothelial cells, endothelial progenitor cells, pericytes, preadipocytes and hematopoietic cells (5) and fat plus platelet rich plasma (PRP), which is claimed to support angiogenesis and reduce absorption of fat (6,7).…”

Section: Prospero Registration Number: Crd42020213407mentioning

confidence: 99%

Protocol for a Systematic Review of Outcome Measurement Tools for Autologous fat Grafting of the Facial Region

Speiser¹,

Almadori²,

Langridge³

et al. 2020

Preprint

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BackgroundAutologous fat grafting has become an established therapeutic entity in several regions of the body. Especially in the facial region, AFG has been widely applied in the field of plastic and reconstructive surgery. Indications include reconstructive purposes such as primary genetic disorders in maxillofacial development, or secondary asymmetries following primary reconstructive surgery. In addition, AFG has found several applications in the field of aesthetic surgery, such as skin rejuvenation. In addition to conventional AFG, recently several alternative strategies of grafting have been developed. Examples for these are transplantation of a graft including adipose derived stem cells (ADSC’s), cell-assisted lipofilling (CAL), and grafting with stromal vascular fraction (SVF). Until today, there is no common consensus on how to measure a successful outcome of autologous graft transplantation in the facial region. This systematic review aims to critically evaluate the current available outcome measurement methods, and compare them with regards to accuracy, cost-effectiveness, safety and validation in the clinical setting. The quality of evidence of included publications will be assessed using the GRADE strategy.MethodsA comprehensive, structured literature search of published articles will be conducted. This is designed by authors and performed in adherence to the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA-P). The literature search will be performed using the electronic databases MEDLINE (PubMed), EMBASE and Cochrane Library, including publications from inception to May 2020. Two independent authors will conduct the search, screen the articles, and complete data collection. The main outcome will be which methods are currently used to determine a successful fat grafting procedure in the face. We propose to review the current methods used to establish their overall performance in several domains. In addition, patients measured via the 3D volumetric photography method will be further analysed with regards to accuracy of the camera and retention of fat per method and ml injected.Discussion Autologous fat grafting in the facial region has gained significant attention in the field of plastic and reconstructive surgery in the past decades. In light of the growing public interest in this topic, a systematic review of the outcome measurement tools is of essence. We will critically evaluate all approaches currently in practice and give a recommendation regarding several aspects of use. A common approach to measurement and reporting of results fosters an environment of high-standard healthcare delivery and promotes future research and informed clinical practice. In addition, we will further analyse the outcomes of different grafting methods using 3D volumetric photography as measurement tool. PROSPERO registration number: CRD42020213407

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The Stromal Vascular Fraction Improves Maintenance of the Fat Graft Volume

Cited by 27 publications

References 32 publications

Phenotypic and Cellular Characteristics of a Stromal Vascular Fraction/Extracellular Matrix Gel Prepared Using Mechanical Shear Force on Human Fat

Phenotypic and Cellular Characteristics of a Stromal Vascular Fraction/Extracellular Matrix Gel Prepared Using Mechanical Shear Force on Human Fat

Стромально-Васкулярна Фракція Жирової Тканини: Перспективи Для Регенеративної Біоінженерії

Protocol for a Systematic Review of Outcome Measurement Tools for Autologous fat Grafting of the Facial Region

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