The Structural and Infected Cell Polypeptides of Influenza B Virus

Abstract

SUMMARYStructural and virus-induced infected cell polypeptides of several strains of influenza B virus were examined by high resolution polyacrylamide gel electrophoresis and shown to be directly analogous to those of the influenza A viruses. Eight structural polypeptides, P1, P2, Ps, HAj, HA2, NA, NP and M were observed in purified virus and at least two additional polypeptides, HA and NS could be detected in infected MDCK cells. The three P proteins plus NP were shown to be associated with RNA-dependent RNA polymerase activity and HA, HA1, HA2 and NA were shown to be glycosylated. Like the influenza A viruses, migrational differences of some of the infected cell polypeptides could be observed between different B strains. Investigation of a time course of virus replication failed to show any temporal control of protein synthesis in the infected cell.

Nucleotides in the panhandle structure of the influenza B virus virion RNA are involved in the specificity between influenza A and B viruses.

“…Preparation of RNP from the A\X-31 virus was as previously described . RNP preparation from B\HK\HG reassortant virus was according to Almond et al (1979) with slight modification. Briefly, concentrated egg-grown virus was dissolved in disruption buffer containing 10 mM Tris-HCl (pH 7n4), 100 mM NaCl, 100 mM KCl, 50 mM MgCl # , 4 mM DTT, 1 % NP40 and 0n5 % lysolecithin.…”

A Comparison of Proteins among Various Influenza B Virus Strains by One-Dimensional Peptide Mapping

“…Racaniello & Palese (1978 have demonstrated the migrational differences of N S 1 and M proteins between the B/Lee/40 and B/Maryland/59 strains. Almond et al (1979) have compared the proteins of four influenza B strains by SDS-PAGE, and have demonstrated that the mobility of NSI, NP and HA proteins are different among strains. In the present paper, we have also found migrational differences in HA, NA and NSI proteins.…”

“…Influenza B virus, like the influenza A virus, consists of the two surface glycoproteins, haemagglutinin (HA) and neuraminidase (NA) and the five internal non-glycosylated proteins, nucleoprotein (NP), matrix (M) protein and three P proteins (P1, P2, Pa) (Almond et al, 1979). The HA glycoproteins may be present in virions as two cleavage products, HA 1 and HA 2.…”

“…The HA glycoproteins may be present in virions as two cleavage products, HA 1 and HA 2. In addition to these structural proteins, two non-structural proteins, NS1 and NS2 can be detected in infected cells (Racaniello & Palese, 1978;Almond et al, 1979;Lamb & Choppin, 1979).…”

Robust Lys63-Linked Ubiquitination of RIG-I Promotes Cytokine Eruption in Early Influenza B Virus Infection

“…Influenza B virus has no distinct subtypes because of homology but is separated into two lineages, Yamagata and Victoria, based on different antigenicities (3). The genome of influenza B virus is composed of eight negative-sense singlestranded RNA segments encoding 11 viral proteins: HA, NA, PA, PB1, PB2, NP, M1, M2, NEP, NB, and NS1 (4,5). Their functions in viral infection cycles have been generally characterized.…”