The structural elucidation and antimicrobial activities of two new monoterpene glucoside derivatives from <i>Lomatogonium carinthiacum</i> (Wulf) Reichb

et al. 2017

Phys Med Rehab Kuror

Background Mongolian medical warm acupuncture is efficacious in treatment of insomnia but the mechanism still remains unclear. There is no research on the effect on the changes in expression of mRNA in the receptors of 5HT, DA, 5HT1α, 5HT2α, and DRD2 and the levels of hormones related to the central HPA axis in the stress response. Objective To further explain the mechanism of Mongolian medical warm acupuncture in insomnia treatment and its role in protecting cerebral neurons, elaborate the effect of Mongolian medical warm acupuncture on the changes in expression of 5HT, DA, 5HT1α, and 5HT2α at different sites in the brains of the PCPA-induced insomnia model rats and the levels of hormones related to the central HPA axis in the stress response. Method 72 Wistar rats were randomly divided into blank group, model group, Mongolian medical warm acupuncture group, and diazepam group of 18 each. The rats were intraperitoneally injected with PCPA (400 mg/kg) for 2 consecutive days to induce insomnia. The sleep latency and sleep duration before and after treatment were observed. The content of 5HT in the hypothalamus and hippocampus was measured with the ELISA method. The content of mRNA in the 5HT1A and 5HT2A receptors in the hypothalamus and hippocampus with the qPCR technique. The content of DA in the hypothalamus and corpus striatum was determined with the ELISA method. The content of ELISA in the hypothalamus and the content of ACTH in the serum of the insomnia rats were determined with the ELISA method. The changes in content of the BCL-2 and BAX proteins in the hippocampus were observed with the immunohistochemical method. Result PCPA intraperitoneal injections were able to prolong the sleep latency of the rats and shorten the sleep duration (P<0.05) when compared with the blank group. Mongolian medical warm acupuncture was able to alleviate the tendency of decreasing weight gain arising from insomnia (P<0.05). Mongolian medical warm acupuncture was able to decrease the levels of CRH and ACTH in the hormones related to central HPA axis in the stress response (P=0.017, P=0.015) and alleviate the overexciting state of the HPA axis arising from insomnia. Mongolian medical warm acupuncture group was able to up-regulate the expression of the BCL-2 protein in the hippocampus, down-regulate the expression of the BAX protein, and increase the BCL-2/BAX ratio (P=0.011). Mongolian medical warm acupuncture was able to protect the hippocampal neurons by changing the content of the apoptosis factors (P=0.024). Conclusion Mongolian medical warm acupuncture decreases the levels of CRH and ACTH in the hormone related to the HPA axis, up-regulates the expression of the BCL-2 protein in the hippocampus, and down-regulates the expression of the BAX protein for improving rat insomnia by influencing the expression of 5HT1A and 5HT2A receptors in the hypothalamus and hippocampus.

Section: Introductionmentioning

confidence: 99%

Research on the Mechanism of Mongolian Medical Warm Acupuncture in Alleviating Insomnia by Increasing the BCL-2/BAX Ratio and Decreasing the Hormones Related to the Central HPA Axis of the Stress Response

et al. 2017

Phys Med Rehab Kuror

“…The structure of the known compound was identified by comparing their spectroscopic data with those reported in the literature (Wang, Dai, & Han, 2014). The structure of the known compound was identified by comparing their spectroscopic data with those reported in the literature (Wang, Dai, & Han, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…In the present study, we carried out a systematic chemical study on the EtOAc extract from the flowers of H. plantaginea, which resulted in the isolation of a new monoterpene glycoside. The structure of the known compound was identified by comparing their spectroscopic data with those reported in the literature (Wang, Dai, & Han, 2014…”

Section: Introductionmentioning

confidence: 99%

Antibacterial effects of two monoterpene glycosides fromHosta plantaginea(lam.) Aschers

Journal of Food Biochemistry

Han

Bao

2016

Self Cite

In this paper, we carried out a systematic chemical study on the ethyl acetate (EtOAc) extract from the flowers of Hosta plantaginea (Lam.) Aschers (H. plantaginea), which resulted in the isolation of a new compound (1) together with one known compound (2). The structure elucidations of new compound were carried out by 1D [1H and 13C nuclear magnetic resonance (NMR)] and 2D‐NMR spectral analysis. Studies on the antibacterial activity established that compound 1 showed statistically higher inhibition against Staphylococcus aureus (S. aureus) compared to streptomycin and compound 2. Among the tested bacteria, Bacillus cereus (B. cereus) was the most sensitive and Yersinia enterocolitica (Y. enterocolitica) was found to be the most resistant. The antibacterial activities exhibited by compound 1 demonstrate their potential for use as nutraceuticals and in food preservation. Practical applications The flowers of H. plantaginea are used as a clinical medicine to treat many diseases, such as swelling, mastadenitis, otitismedia, anabrosis, lung heat, and terrific heat. Various pharmacological properties have been observed in some steroidal glycosides and alkaloids from H. plantaginea including cytotoxic and anti‐inflammatory. In the article, the antibacterial activities of two monoterpene glycosides from the flowers of H. plantaginea have been evaluated for the first time. Therefore, the results may have some useful practical implications at both the food and medicine technology.

“…Terpenoids and flavonoids (Massia et al 1981;Jia and Yuan 2011) have been reported from L. carinthiacum. In our previous studies, flavonoids (Bao et al 2014), two new monoterpene glucoside derivatives (Wang, Dai, et al 2014), two new xanthones (Wang, Bao, et al 2014), and a biflavonoid glycoside (Wang et al 2015) were isolated from the CHCl 3 , EtOAc and n-BuOH crude extract of this plant. In the continuation of our work on the constituents of the n-BuOH crude extract from L. carinthiacum, three biflavonoid glycosides were isolated.…”

Section: Introductionmentioning

confidence: 99%

The isolation and structural elucidation of three new biflavonoid glycosides fromLomatogonium carinthiacum

Bao

Natural Product Research

et al. 2015

Self Cite