The structure-forming role of magnesium pyrophosphate in the formation of DNA-containing microparticles during PCR

Danilevich, V. N.; Мачулин, А. В.; Sorokin, V. V.; Mulyukin, A. L.

doi:10.1134/s1607672915040171

Cited by 2 publications

(1 citation statement)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We further observed, upon prolonged replication (6–12 h), the emergence of spherical particles of <3 μm in diameter in the reaction mixture (Figure C). As reported before, these nanoparticles are co‐precipitates of DNA and magnesium pyrophosphate that accumulates as the byproduct of DNA synthesis in the absence of pyrophosphate kinase . Consistent with this, the observed nanoparticles could be stained by DNA‐binding dye (Figure S3).…”

Section: Resultssupporting

confidence: 86%

Reconstitution and Coupling of DNA Replication and Segregation in a Biomimetic System

et al. 2019

View full text Add to dashboard Cite

A biomimetic system capable of replication and segregation of genetic material constitutes an essential component for the future design of a minimal synthetic cell. Here we have used the simple T7 bacteriophage system and the plasmid‐derived ParMRC system to establish in vitro DNA replication and DNA segregation, respectively. These processes were incorporated into biomimetic compartments providing an enclosed reaction space. The functional lifetime of the encapsulated segregation system could be prolonged by equipping it with ATP‐regenerating and oxygen‐scavenging systems. Finally, we showed that DNA replication and segregation processes could be coupled in vitro by using condensed DNA nanoparticles resulting from DNA replication. ParM spindles extended over tens of micrometers and could thus be used for segregation in compartments that are significantly longer than bacterial cell size. Overall, this work demonstrates the successful bottom‐up assembly and coupling of molecular machines that mediate replication and segregation, thus providing an important step towards the development of a fully functional minimal cell.

show abstract

Section: Resultssupporting

confidence: 86%