The Structure of Infectious Pancreatic Necrosis Virus RNA

Abstract

SUMMARYThe RNA from infectious pancreatic necrosis virus has been purified and had a sedimentation velocity of I4S on sucrose gradients, a buoyant density of 1.6o g/ml in Cs2SO 4 and pyrimidine to purine ratios near unity. The RNA had the appearance of a linear double stranded molecule with an average length of 0"92 #m and a standard deviation of 0.07 #m when observed under the electron microscope using the Kleinschmidt protein film technique. This would correspond to a mol. wt. of 2. 4 + 0.2 × IO 6. The RNase A resistance of IPN virus RNA exhibited a marked salt dependence ; it was 92 % resistant in o. I M-NaC1, but only 9 % resistant, or less, in o.oI M-NaC1. The RNA was resistant to denaturation by boiling at NaC1 concentrations of o'04 M or higher, but did denature at lower concentrations.Polyacrylamide gel electrophoresis of the RNA indicated that two RNA species were present and the standard deviation of lengths in the electron microscope indicated that they could not differ by more than 4 × IO5 in mol. wt.

Isolation of infectious pancreatic necrosis virus (serotype Ab) from diverse species of estuarine fish

“…Virus purification and antiserum production Virus was concentrated by polyethylene glycol precipitation (Macdonald & Yamamoto, 1977) and purified on linear 10-50 % sucrose gradients by centrifugation at 97 000 x g for 45 min at 4 °C. The virus band was removed by side puncture, dialyzed overnight against TNE buffer (0.2 M tris, 0.15 M NaC1, 0.001 M Na2EDTA; pH 7.2), and frozen at -70 °C.…”

Studies on the Mechanism of Temperature Sensitivity of Infectious Pancreatic Necrosis Virus Replication

“…Virions are unenveloped icosahedrons, approximately 60 nm in diameter, which contain two segments of 14S doublestranded RNA (dsRNA) with molecular weights of 2.5 x 106 and 2.3 x 106 (Dobos, 1976;Macdonald & Yamamoto, 1977). On the basis of virion morphology and genome size and structure, IPNV is representative of a yet unclassified group of viruses which also includes infectious bursal disease virus of chickens and Drosophila X virus (Dobos et al, 1979).…”

Reponse immunitaire humorale induite par le virus de la necrose pancreatique infectieuse (VNPI) chez la truite mouchetee (Salvelinus fontinalis L ) et caracterisation de l immunoglobuline produite

“…Elle se révèle être de 1,33 g/cm 3 (Dobos, 1976;MacDonald et Yamamoto, 1977). Le virion possède un coefficient de sédimentation de 435 S et un poids moléculaire de 55 X 10 6 daltons (Dobos e_taL,1977).…”

“…Les deux segments sont de dimensions différentes avec des poids moléculaires de 2,5 X 10 6 et de 2,3 X 10"6 daltons (Dobos, 1976;MacDonald et Yamamoto, 1977 sur des cellules RTG-2, ainsi que la multiplication cellulaire (Malsberger et Cerini, 1965). …”