The Structure of Magnamycin

Cloning and Nucleotide Sequences of Two Genes Involved in the 4″-O-Acylation of Macrolide Antibiotics fromStreptomyces thermotolerans

“…of the procedures are described in Materials and Methods. Various streptomycetes used in this analysis (lane [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] are summarized in Table. the 4" -O-acylation of macrolide antibiotics could be expressed in S. lividans when recombined with the shuttle vector pUJ350. S. lividans transformants could convert exogenously added tylosin to 4"-O-acyltylosins.…”

“…1) is one of the most industrially important antibiotics. Okamoto et al 5) showed that AIV was efficiently produced by bioconversion of tylosin using a mutant strain of the carbomycin 6 ) producer Streptomyces thermotolerans in which the carbomycin-lactone formation was blocked but the acyltransferase activity was intact. Based on this bioconversion method, the production of AIV had been developed on an industrial scale.…”

The Quest for Quinine: Those Who Won the Battles and Those Who Won the War

“…Woodwards genius contributed to the deduction of the structures of penicillin (1945), [107] patulin (1949), [108] strychnine (1947), [109] oxytetracycline (1952), [110] carbomycin (magnamycin, 1953), [111] cevine (1954), [112] gliotoxin (1958), [113] calycanthine (1960), [114] oleandomycin (1960), [115] streptonigrin (1963), [116] and tetrodotoxin (1964), [117] as well as others. [118] He unveiled the family of macrolide antibiotics, for which he also proposed a mode of formation in nature [119] -as he had done with the first proposal of the cyclization of squalene in cholesterol biosynthesis. [120] The scientific world first knew Woodward through a series of publications (1940)(1941)(1942) highlighting the correlation of ultraviolet spectra with molecular structure.…”

Biosynthesis

“…Subsequently the structure of magnamycin A was revised by KUEHNE et al (1965) and WOODWARD et al (1965). WOODWARD (1957) first proposed an 18-membered lactone ring for the structure of magnamycin and suggested that its lactone ring moiety originates from nine acetate and one methionine unit.…”