The Structure of NRRL B-512 Dextran. Methylation Studies<sup>2</sup>

Cleve, J. W. Van; Schaefer, W.; Rist, C. E.

doi:10.1021/ja01598a064

Cited by 127 publications

(33 citation statements)

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“…In the latter case, dextran-based gels are used as a delivery system for the specific targeting of drugs to the colon, where they are degraded by a secreted bacterial dextranase. The L. mesenteroides NRRL B-512F dextran polymer is water-soluble, and consists of 95% a-(1fi6) linkages in the main chain, and 5% a-(1fi3)-linked single unit side chains (van Cleve et al, 1956). Its biosynthesis is mediated by dextransucrase DSRS (EC 2.4.1.5), which is a 1527 amino-acid glucosyltransferase (Wilke-Douglas et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Production of dextran in transgenic potato plants

et al. 2005

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The production of dextran in potato tubers and its effect on starch biosynthesis were investigated. The mature dextransucrase (DsrS) gene from Leuconostoc mesenteroides was fused to the chloroplastic ferredoxin signal peptide (FD) enabling amyloplast entry, which was driven by the highly tuber-expressed patatin promoter. After transformation of two potato genotypes (cv. Kardal and the amylose-free (amf) mutant), dextrans were detected by enzyme-linked immunosorbent assay (ELISA) in tuber juices of Kardal and amf transformants. The dextran concentration appeared two times higher in the Kardal (about 1.7 mg/g FW) than in the amf transformants. No dextran was detected by ELISA inside the starch granule. Interestingly, starch granule morphology was affected, which might be explained by the accumulation of dextran in tuber juices. In spite of that, no significant changes of the physicochemical properties of the starches were detected. Furthermore, we have observed no clear changes in chain length distributions, despite the known high acceptor efficiency of DSRS.

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Section: Introductionmentioning

confidence: 99%

Production of dextran in transgenic potato plants

et al. 2005

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“…Three known dextrans are the most similar to linear α-1, 6-glucan obtained in present study. One of them is the water soluble dextran produced by Leuconostoc mesenteroides NRRL B-512, which have a molecular weight of 8 x 10 7 Da and contain 95% linear α-(1→6) linkages and 5% α-(1→3) linkages (Van Cleve et al, 1956); the latter are branch points for side chains including no more than two glucose units. Another one is dextran from Weissella confuse, strains E392 (Maina et al, 2008) and LBAE C39-2 (Amari et al, 2013) that contains only 2.7% -2.8% α-(1→3)-linked branches.…”

Section: Dextrans From Other Microorganisms and Their Immunological Pmentioning

confidence: 99%

Glycopolymers from Saccharomyces Cerevisiae BIM Y-195 with Unusual Immunochemical Properties: Isolation, Structural Identification and Prediction of Their Role in Pathogenesis/Treatment of Autoimmune Thyroid Diseases

Kiseleva¹,

Mikhailopulo²,

Новик³

et al. 2014

IMMU

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Molecular mimicry in the broad sense is structural, functional or immunologic similarity between unrelated macromolecules (Oldstone, 1998). In biomedical studies this phenomenon is discussed as the most proven mechanism of triggering/prevention of autoimmune diseases (Kivity et al., 2009). In this paper, we isolate and structurally identify the yeast biopolymers (BPs) that selectively bind human autoantibodies to thyroid peroxidase (TPO) and thyroglobulin (Tg) (anti-TPO and anti-Tg, respectively), e.g. immunologically mimic thyroid antigens. The BPs, viz., BPanti-TPO and BPanti-Tg, were isolated from the soluble fraction of S. cerevisiae BIM Y-195 by affinity chromatography with anti-TPO or anti-Tg, respectively. Both affinity eluates, AEanti-TPO and AEanti-Tg, showed functional activity characteristic of BPanti-TPO and BPanti-Tg, viz, ability (i) to distinquish anti-TPO (anti-Tg) from other IgG and (ii) to compete with TPO (Tg) for binding of anti-TPO (anti-Tg) in ELISA tests. The semi-preparative size exclusion chromatography of AEanti-TPO and AEanti-Tg with detection by refractometer gave a 5000-7000 Da fractions containing pure BPanti-TPO and BPanti-Tg, respectively, according to ELISA data. Analysis by two-dimensional NMR spectroscopy including 1 H, 1 H COSY and 1 H, 13 C HSQC experiments indicated that both substances are linear α-1, 6-glucans. It was shown for the first time an immunological similarity (molecular mimicry) of α-1, 6-glucans of S. cerevisiae BIM Y-195 and human thyroid proteins, TPO and Tg, just as it was shown early for α-1, 6-glucans of Bifidobacterium bifidum BIM B-733D and TPO/Tg (Kiseleva et. al., 2013). On the whole, our data point to a possible role of wine yeast in the pathogenesis/treatment of autoimmune thyroid diseases (ATD).

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“…B-512 (Sigma). It is known that dextran is a branched D-glucan linked predominantly by ~-1-~6 glycoside bonds, and ~-1-~ 3 and other branch linkages are contained depending on the type of dextran [9][10][11][12][13]. Therefore, it is expected that dextran sulfate is also branched.…”

Section: Physical Properties Of Dextran Sulfatesmentioning

confidence: 99%

Ion chromatography using anion-exchangers modified with dextran sulfate

et al. 1999

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SummaryRetention behavior of anions and cations on anion-exchangers modified with dextran sulfate has been investigated. Retention of anions was remarkably reduced by the modification, and the retention factor decreased with decreasing eluent concentration when sodium sulfate was used as the eluent. Cations were also retained on the modified stationary phase, and alkali and alkalineearth metal ions were separated using copper sulfate or tris(2,2'-bipyridyl)ruthenium chloride as eluent. The size of the dextran sulfate strongly affected the retention behavior of analyte ions.

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The Structure of NRRL B-512 Dextran. Methylation Studies²

Cited by 127 publications

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Production of dextran in transgenic potato plants

Production of dextran in transgenic potato plants

Glycopolymers from Saccharomyces Cerevisiae BIM Y-195 with Unusual Immunochemical Properties: Isolation, Structural Identification and Prediction of Their Role in Pathogenesis/Treatment of Autoimmune Thyroid Diseases

Ion chromatography using anion-exchangers modified with dextran sulfate

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The Structure of NRRL B-512 Dextran. Methylation Studies2

Cited by 127 publications

References 0 publications

Production of dextran in transgenic potato plants

Production of dextran in transgenic potato plants

Glycopolymers from Saccharomyces Cerevisiae BIM Y-195 with Unusual Immunochemical Properties: Isolation, Structural Identification and Prediction of Their Role in Pathogenesis/Treatment of Autoimmune Thyroid Diseases

Ion chromatography using anion-exchangers modified with dextran sulfate

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The Structure of NRRL B-512 Dextran. Methylation Studies²