The Structure of the Capsular Polysaccharide from Klebsiella K-Type 21

Abstract

Methylation, periodate oxidation, and partial hydrolysis studies on the capsular polysaccharide, and on the carboxyl reduced polynler, of Klebsiella K21 show the structure to consist of a repeating unit.The anomeric linkages were determined by p.m.r. spectroscopy of isolated oligosaccharides and, in part, by specific enzymes. P.m.r. spectroscopy of the original polysaccharide in methyl sulfoxide-d, showed clearly a ratio of one pyruvic acid ketal (CH,, T 8.5) to five anomeric protons (T 4.65-5.40).Les methodes de methylation, oxydation periodique et hydrolyse partielle portees au polysaccharide capsulaire, et au polyn~ere avec le groupe carboxyle reduit, de Klebsiella K21 ont dCmontre que la structure se compose d'une unite qui se repete.Les liaisons anomeres ont Cte distinguees par la spectroscopie r.m.n. des oligosaccharides isoles et, en partie, par des enzymes specifiques. La spectroscopie i.m.n. du polysaccharide original dans le methyl sulfoxide-d, a etabli le rapport un a cinq entre le cetal de l'acide pyruvique (CH,. r 8.5) et les protons anomeres (t 4.65-5.40). Can. J. Chem.. 51, 198 (1973)

The Structure of the Capsular Polysaccharide of Klebsiella K-type 5

“…Approxiinately 80 different serotypes are recognized and Nimmich (1, 2) has provided qualitative analyses of these capsular materials. In continuation of our studies (3,4) on the structure of these polysaccharides we now report on the capsule from Klebsiella K-type 5. This polysaccharide differs from those previously described in this genus by lacking any carbohydrate side chain.…”

Human monoclonal macroglobulins with specificity for Klebsiella K polysaccharides that contain 3,4-pyruvylated-D-galactose and 4,6-pyruvylated-D-galactose.

“…The following polysaccharides were studied: commercial Bacto and special agar (Difco Laboratories, Detroit, Mich.) and successive fractions prepared from it, as described by Duckworth and Yaphe (18), by extraction with water at 20 and 50°C, and the residue; commercial agarose (Fisher Scientific Co., Pittsburgh, Pa.); galactans from the snails Helix pomatia (19)(20)(21)(22); Arianta arbustorum and their first stages of partial periodate oxidation and Smith degradation (23) Cepaea nemoralis and Biomphalaria glabrata (24); Larch arabinogalactan (25) and pig pneumogalactan (26,27) from Dr. Niel Glaudemans (National Institutes of Health, Bethesda, Md. ); polysaccharides from Klebsiella K7 (28), K21 (29), and K32 (30), Rhizobiura trifolii TA 1 (31), and types IV, XIV, and XXXII pneumococcal polysaccharides (32) (37), a heparin analogue from alginic acid (38), and chondroitin sulfate (Sigma Chemical Co., St. Louis, Mo. ); dextran (39) and various blood group B and I substances (40)(41)(42)(43)(44).…”

The Occurrence of α(1 → 2) Linked N‐Acetylperosamine‐Homopolymer in Lipopolysaccharides of Non‐O1 Vibrio cholerae Possessing an Antigenic Factor in Common with Ol V. cholerae

“…The 1,2-substitutions were indicated by a shift of the resonances of C-1 (101.33 ppm) and C-2 (77.86 ppm) to low fields compared to those in a-N-acetylperosamine (12) (C-1, 94.75 ppm; C-2, 70.70 ppm). The 1.11-NMR spectrum of Hakata PS was also similar to that of the Nacetylated PS from 01 V. cholerae, in which the signals were observed at 1.06 ppm (broad d, 3H, H-6), 1.91 ppm (broad s, -COCH3) and 5.02 ppm (broad s, 1H, H-1) which is characteristic of the anomeric hydrogen of a-D-mannopyranosyl linkage (4). A signal in the anomeric region of the 1-11-and 13C-NMR spectra of Hakata PS indicates that a single type of glycosyl linkage is present in the 0-specific chain of Hakata P-LPS molecules.…”