2022
DOI: 10.1002/prot.26326
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The structure of the Clostridium thermocellum RsgI9 ectodomain provides insight into the mechanism of biomass sensing

Abstract: Clostridium thermocellum is actively being developed as a microbial platform to produce biofuels and chemicals from renewable plant biomass. An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surfacedisplayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes. Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense differen… Show more

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Cited by 6 publications
(6 citation statements)
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References 75 publications
(171 reference statements)
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“…The C-terminal part of these RsgIs has a divergent carbohydrate-binding module (CBM), which was proposed to sense different types of polysaccharides (27)(28)(29). In Clostridium thermocellum, RsgI1, RsgI2, and RsgI4 contain family 3b CBMs that target cellulose; RsgI3 contains a tandem protective antigen 14 (PA14) motif that recognizes pectin; RsgI5 contains a family 42 CBM that binds to arabinose; RsgI6 contains a family 10 glycosidase hydrolase that targets both xylan and cellulose; RsgI7 and RsgI8 do not contain an obvious CBM, and their functions are currently unknown; and lastly, RsgI9 contains a protease-like domain that is capable of binding cellulose (28)(29)(30). After signal transduction across the cell membrane, the RsgIs release their bound cognate SigIs for initiating transcription of a subset of cellulosomal genes, resulting in the dynamic regulation of cellulosome function, which is one of the major reasons for the high efficiency of anaerobic cellulosome-producing bacteria in lignocellulose degradation (11,(24)(25)(26).…”
Section: Introductionmentioning
confidence: 99%
“…The C-terminal part of these RsgIs has a divergent carbohydrate-binding module (CBM), which was proposed to sense different types of polysaccharides (27)(28)(29). In Clostridium thermocellum, RsgI1, RsgI2, and RsgI4 contain family 3b CBMs that target cellulose; RsgI3 contains a tandem protective antigen 14 (PA14) motif that recognizes pectin; RsgI5 contains a family 42 CBM that binds to arabinose; RsgI6 contains a family 10 glycosidase hydrolase that targets both xylan and cellulose; RsgI7 and RsgI8 do not contain an obvious CBM, and their functions are currently unknown; and lastly, RsgI9 contains a protease-like domain that is capable of binding cellulose (28)(29)(30). After signal transduction across the cell membrane, the RsgIs release their bound cognate SigIs for initiating transcription of a subset of cellulosomal genes, resulting in the dynamic regulation of cellulosome function, which is one of the major reasons for the high efficiency of anaerobic cellulosome-producing bacteria in lignocellulose degradation (11,(24)(25)(26).…”
Section: Introductionmentioning
confidence: 99%
“…Coupled with prior findings that show RsgI9's C-terminal ectodomain binds cellulose, these results are consistent with it regulating the composition of the cellulosome via a RIP signal transduction mechanism. 22 In this process, polysaccharide binding to the RsgI9 domain would cause the pre-cleaved CRE domain to separate, thereby exposing it to degradation by the RasP intramembrane metalloprotease to release a yet to be identified σ-factor that controls gene transcription. This work builds upon previous studies of the CRE domain by providing insight into the potential mechanism for autoproteolysis.…”
Section: A Conserved Hydrogen-bond Network May Impart Strain Onto The...mentioning
confidence: 99%
“…[16][17][18][19] In the signaling process for the SigItype σ-factors, each of the membrane-bound anti-σ factors are believed to bind to different types of extracellular polysaccharides. 17,[20][21][22] They then transduce this signal across the membrane, triggering the release of their cognate SigI-type σ-factor from the cytoplasmic membrane. 23 The SigI-type σ-factor then binds to the RNA polymerase complex enabling it to transcribe specific GH encoding genes, as well as genes that encode scaffoldin proteins used to construct the cellulosome.…”
Section: Introductionmentioning
confidence: 99%
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