The Study of Ginger-Derived Extracellular Vesicles as a Natural Nanoscale Drug Carrier and Their Intestinal Absorption in Rats

Man, Fulong; Chen, Meng; Liu, Yang; Wang, Yuchen; Zhou, Yun; Ma, Jinqian; Lü, Rong

doi:10.1208/s12249-021-02087-7

Cited by 37 publications

(27 citation statements)

References 46 publications

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“…Given this presumption, another study demonstrated sunflower-derived EV uptake by the ascomycete Sclerotinia sclerotiorum through reduced hyphae growth and spore germination [43], indicating that fungal uptake of plant EVs is possible in principle. In addition, recent studies indicated in vitro uptake of plant-derived (ginger, grapefruit, pineapple, and paprika) EVs and VLNs in human and rat cells [15,22,34], which is of great scientific interest due to their therapeutic potential in nanomedicine [11]. However, whether this holds for other fungal plant pathogens remains to be verified.…”

Section: Discussionmentioning

confidence: 99%

Extracellular vesicles isolated from dsRNA-sprayed barley plants exhibit no growth inhibition or gene silencing in Fusarium graminearum

Schlemmer

Lischka

Wegner

et al. 2022

Fungal Biol Biotechnol

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Numerous reports have shown that incorporating a double-stranded RNA (dsRNA)-expressing transgene into plants or applying dsRNA by spraying it onto their leaves successfully protects them against invading pathogens exploiting the mechanism of RNA interference (RNAi). How dsRNAs or siRNAs are transferred between donor host cells and recipient fungal cells is largely unknown. It is speculated that plant extracellular vesicles (EVs) function as RNA shuttles between plants and their pathogens. Recently, we found that EVs isolated from host-induced gene silencing (HIGS) or spray-induced gene silencing (SIGS) plants contained dsRNA-derived siRNAs. In this study, we evaluated whether isolated EVs from dsRNA-sprayed barley (Hordeum vulgare) plants affected the growth of the phytopathogenic ascomycete Fusarium graminearum. Encouraged by our previous finding that dropping barley-derived EVs on F. graminearum cultures caused fungal stress phenotypes, we conducted an in vitro growth experiment in microtiter plates where we co-cultivated F. graminearum with plant EVs isolated from dsRNA-sprayed barley leaves. We observed that co-cultivation of F. graminearum macroconidia with barley EVs did not affect fungal growth. Furthermore, plant EVs containing SIGS-derived siRNA appeared not to affect F. graminearum growth and showed no gene silencing activity on F. graminearum CYP51 genes. Based on our findings, we concluded that either the amount of SIGS-derived siRNA was insufficient to induce target gene silencing in F. graminearum, indicating that the role of EVs in SIGS is minor, or that F. graminearum uptake of plant EVs from liquid cultures was inefficient or impossible.

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Section: Discussionmentioning

confidence: 99%

Extracellular vesicles isolated from dsRNA-sprayed barley plants exhibit no growth inhibition or gene silencing in Fusarium graminearum

Schlemmer

Lischka

Wegner

et al. 2022

Fungal Biol Biotechnol

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show abstract

“…While another study convincingly demonstrated sunflower-derived EV uptake by the ascomycete Sclerotinia sclerotiorum through reduced hyphae growth and spore germination (Regente et al, 2017), whether this holds true for other fungi or other pathosystems remains unknown. Notably, the latest studies demonstrated in vitro uptake of plant-derived (ginger, grapefruit, pineapple and paprika) EVs in human and rat cells (Garaeva et al, 2021;Ito et al, 2021;Man et al, 2021), which is of great scientific interest due to their therapeutic potential in nanomedicine (Alfieri et al, 2021). Studies on CYP3RNA-expressing Arabidopsis (HIGS) plants revealed a loss of CYP3RNA-mediated Fg resistance in ESCRT-III mutants (Schlemmer et al, 2021a).…”

Section: Discussionmentioning

confidence: 99%

Extracellular vesicles isolated from dsRNA sprayed barley plants exhibit no growth inhibition or gene silencing in Fusarium graminearum

Schlemmer

Lischka

Biedenkopf

et al. 2021

Preprint

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Incorporating a double-stranded RNA (dsRNA)-expressing transgene into plants or applying dsRNA by spraying it onto plant leaves successfully protects plants against invading pathogens with RNA interference (RNAi). How dsRNAs or siRNAs are transferred between donor host cells and recipient fungal cells is largely unknown It is speculated that plant extracellular vesicles (EVs) function as RNA shuttles between plants and their interacting pathogens. Recently, we found that EVs isolated from HIGS or SIGS plants contained dsRNA-derived siRNAs. In this study, we evaluated whether isolated EVs from RNA-sprayed barley ( Hordeum vulgare ) plants affected the growth of the phytopathogenic ascomycete Fusarium graminearum ( Fg ). Encouraged by our previous finding that dropping barley-derived EVs on Fg cultures caused fungal stress phenotypes, we conducted an in vitro growth experiment in microtiter plates where we co-cultivated Fg with plant EVs isolated from dsRNA-sprayed barley leaves. We observed that co-cultivation of Fg macroconidia with barley EVs did not affect fungal growth. Furthermore, plant EVs containing SIGS-derived siRNA appeared not to affect Fg growth and showed no gene silencing activity on FgCYP51 genes. We conclude that either the amount of spray-derived sRNA was insufficient to induce target gene silencing (SIGS) in Fg or Fg uptake of plant EVs from liquid cultures was inefficient or impossible.

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“…PDNVs contain a broad range of naturally occurring metabolites, which may contribute to therapeutic effects or health-promoting effects of PDNVs: ginger-derived nanovesicles contain highly enriched 6-gingeraol, 8-gingerol, 10-gingerol, and 6-shogaol compared with ginger slices [ 34 , 49 ] and depletion of the nanovesicles from ginger extracts markedly reduced shogaol detected by thin-layer chromatography method [ 35 ]; broccoli-derived nanovesicles contain sulforaphane [ 50 ] and HPLC revealed that sulforaphane is more enriched in nanoparticles than in microparticles, while broccoli extracts have little sulforaphane in free form; grapefruit-derived nanovesicles contain naringenin [ 51 ]; citrus limon and strawberry-derived nanovesicles contain vitamin C [ 16 ]; edible tea flower-derived nanovesicles are enriched in epigallocatechin gallate, epicatechin gallate, epicatechin, vitexin, myricetin-3- O -rhamnoside, kaempferol-3- O -galactoside, and myricetin; and nanovesicles from citrus limon contain citrate and vitamin C [ 52 ]. Furthermore, oat-derived nanovesicles contained a five-times greater percentage of beneficial fiber β-glucan than in oat flour [ 29 ] and lemon-derived nanovesicles contained galacturonic acid-enriched pectin-type polysaccharide as the active factor [ 53 ], suggesting PDNVs could be developed as nano-size formulated prebiotics.…”

Section: Characteristics Of Unmodified Pdnvsmentioning

confidence: 99%

Emergence of Edible Plant-Derived Nanovesicles as Functional Food Components and Nanocarriers for Therapeutics Delivery: Potentials in Human Health and Disease

Kim

2022

Cells

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Extracellular vesicles (EVs) are a highly heterogeneous population of membranous particles that are secreted by almost all types of cells across different domains of life, including plants. In recent years, studies on plant-derived nanovesicles (PDNVs) showed that they could modulate metabolic reactions of the recipient cells, affecting (patho)physiology with health benefits in a trans-kingdom manner. In addition to its bioactivity, PDNV has advantages over conventional nanocarriers, making its application promising for therapeutics delivery. Here, we discuss the characteristics of PDNV and highlight up-to-date pre-clinical and clinical evidence, focusing on therapeutic application.

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The Study of Ginger-Derived Extracellular Vesicles as a Natural Nanoscale Drug Carrier and Their Intestinal Absorption in Rats

Cited by 37 publications

References 46 publications

Extracellular vesicles isolated from dsRNA-sprayed barley plants exhibit no growth inhibition or gene silencing in Fusarium graminearum

Extracellular vesicles isolated from dsRNA-sprayed barley plants exhibit no growth inhibition or gene silencing in Fusarium graminearum

Extracellular vesicles isolated from dsRNA sprayed barley plants exhibit no growth inhibition or gene silencing in Fusarium graminearum

Emergence of Edible Plant-Derived Nanovesicles as Functional Food Components and Nanocarriers for Therapeutics Delivery: Potentials in Human Health and Disease

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