The Study on Poly-Lysine-Alginate Microcapsules Mediated Virus Genomic DNA Transfection

Abstract

The goal in this paper is to investigate the efficiency of poly-PLL (Poly-L-lysine)/ Alg (Alginate) vector mediated virus genomic DNA transfection and the virus genomic DNA’s biological activity in vivo. After Pseudorabies virus (PRV) genomic DNA being adhered to the porous СаСО3 particles, PLL and Alg were alternately polymerized on the surface of the porous DNA-СаСО3 particles to 7 layers, which were later dissolved them by EDTA to remove СаСО3 cores; the vectors in which the DNA were coated by poly-PLL/Alg, were harvested to infect the rabbits and observe the replication of viral DNA. Porous СаСО3 particles, which were obtained from the reaction between Na2CO3 and CaCl2, had an efficiency of absorbing DNA 1 µg/mg СаСО3 particles. After being coated by PLL/Alg, microcapsules were obtained with the diameter of 2-4 µm. 10.0 µg of poly-PLL/Alg-PRV DNA microcapsules could cause rabbits’ death by intramuscular injection. The identification of PCR shows that the death was caused by PRV infection. The results indicate that Poly-PLL/Alg microcapsules can mediate efficient transfection of DNA.

Surface-Modified Biopolymer Microparticles: A Potential Surrogate for Studying Legionella pneumophila Attachment to Biofilms in Engineered Water Systems

“…The microparticles were harvested by centrifugation at 6000 g for 10 min, and they were washed twice with deionized water to remove the excess alginate. To load the DNA marker by passive adsorption in step 2, 10 12 –10 16 copies of the plasmid-free, 352 base pair, double-stranded synthetic DNA tracer, K3, and the microparticles (0.33 mg/mL) were incubated overnight on a magnetic stirrer . The DNA-loaded microparticles were harvested by centrifugation, washed using deoxyribonuclease- and ribonuclease-free distilled water, and then resuspended in deionized water.…”

“…To load the DNA marker by passive adsorption in step 2, 10 12 −10 16 copies of the plasmid-free, 352 base pair, double-stranded synthetic DNA tracer, 13 K3, and the microparticles (0.33 mg/mL) were incubated overnight on a magnetic stirrer. 19 The DNA-loaded microparticles were harvested by centrifugation, washed using deoxyribonuclease-and ribonuclease-free distilled water, and then resuspended in deionized water. In step 3, the microparticles underwent surface modification by layer-bylayer (LbL) self-assembly.…”

Evaluation of Biopolymer Materials and Synthesis Techniques to Develop a Rod-Shaped Biopolymer Surrogate for Legionella pneumophila

“…CaCO 3 microparticles without and with additives: CaCO 3 microparticles were produced through precipitation [23] by adding 10 mL of 0.35 M Na 2 CO 3 to an equal volume of 0.35 M CaCl 2 under stirring at 27,500 rpm (Omni tissue homogenizer TH115-K). The solution was stirred for 30 s after the addition of Na 2 CO 3 , incubated at room temperature without stirring for 10 min, and centrifuged at 2500 rpm for 5 min to obtain CaCO 3 microparticles.…”