The subcutaneous air pouch model of synovium and the inflammatory response to heat aggregated gammaglobulin

Abstract

Subcutaneous injection of sterile air in rodents results in the formation of an air pouch with a lining morphologically similar to synovium (Edwards et al., 1981). We extended the comparison between pouch and synovial tissue and confirmed broad similarities in structure and function but also noted important differences. The air pouch was used to study the time course of the acute inflammatory response to heat aggregated human IgG. Saline washout of the pouch allowed simultaneous measurement of cellular and mediator components of the inflammatory exudate. The aggregates were rapidly phagocytosed by the pouch lining cells, resulting in acute inflammation characterised by polymorphonuclear leucocyte infiltration with peak numbers in the exudate at 12 hours, temporally dissociated from the earlier peak of PGE2 at 3 hours.

The effects of 5HT3 receptor antagonist granisetron on inflammatory parameters and angiogenesis in the air-pouch model of inflammation

“…There was massive recruitment of cells into the pouches of animals injected with carrageenan after 6 h (from 1 × 10 7 to 11.71 × 10 7 ). At this time point, in carrageenan-treated pouches, the cells were mostly PMN and changed to a prevalence of macrophages from 48 h onwards (data not shown), as it has been reported previously [29,30]. The total number of infiltrating leukocytes in the pouch fluid was 11.16 × 10 7 at hour 72 (Fig.…”

Amitriptyline, clomipramine, and maprotiline attenuate the inflammatory response by inhibiting neutrophil migration and mast cell degranulation

“…The rat air pouch is a cavity that differs from others, such as the peritoneum, by its absence of mast cells. 23 We intended to investigate whether the tested antidepressant drugs would still produce an anti-inflammatory response in the absence of these cells. We demonstrated that the effects of both carrageenan and the direct chemotactic factor fMLP were markedly inhibited by amitriptyline, clomipramine, and maprotiline.…”

Long-term ethanol intoxication reduces inflammatory responses in rats

“…A sign of withdrawal (convulsions on handling) here detected appeared to reach a peak at 7 h, but high values were also obtained between 6 and 11 h (data not shown), a result closely similar to the findings of previous reports (14,18). Although these results indicated that the dose was sufficiently high to produce dependence on ethanol, they do not allow the conclusion that there is a relationship between abstinence duration and changes in It has been demonstrated that carrageenan-induced neutrophil migration occurs by a process dependent on the release of chemotactic mediators by resident cells (25,26). Thus, it is possible that the inhibitory effect of ethanol on carrageenan-induced neutrophil recruitment could occur by an inhibitory action on the release of chemotactic agents such as leukotriene B 4 , TNF-α, interleukin 1 or 8 from macrophages and/or mast cells.…”