The substrate specificity of a glucoamylase with steroidal saponin-rhamnosidase activity from Curvularia lunata

Feng, Bing; Kang, Liping; B, Ma; Quan, Bo; Zhou, Wenbin; Wang, Yongze; Yu, Zhao; Liu, Yi‐Xun; Wang, Shengqi

doi:10.1016/j.tet.2007.04.076

Cited by 33 publications

(18 citation statements)

References 18 publications

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“…Previous investigations showed that although the glucosyl residue at the C-26 position of furostanosides is readily biotransformed into the corresponding spirostanosides, the glycosyl at C-3 of steroidal saponins is difficult to hydrolyse (Feng et al 2007b). Therefore, recent attempts have been made to solve this problem.…”

Section: Introductionmentioning

confidence: 99%

Production of diosgenin from yellow ginger (Dioscorea zingiberensis C. H. Wright) saponins by commercial cellulase

Liu

Huang

Sun

et al. 2009

World J Microbiol Biotechnol

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A commercial cellulase was first assessed to be effective in hydrolyzing glycosyl at the C-3 and C-26 positions in steroidal saponins from yellow ginger (Dioscorea zingiberensis C. H. Wright) to diosgenin, a very important chemical in the pharmaceutical industry. The effect of different parameters on enzyme hydrolysis was further investigated by systematically varying them. The highest yield was achieved when the hydrolysis ran at 55°C and pH 5.0 with an enzyme to substrate ratio of 15 × 10(3) U/g. The biotransformed products identified using TLC and HPLC confirmed that the cellulase was capable of releasing diosgenin from steroidal saponins. Moreover, the biotransformation process was explored by LC-MS and LC-MS/MS analysis. Enzymatic hydrolysis together with 40 % of the original sulphuric acid used increased the diosgenin yield by 15.4 ± 2.7% than traditional method. Therefore, the commercial cellulase may serve as a promising tool for industrial diosgenin production and for further use in saponin modification.

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Section: Introductionmentioning

confidence: 99%

Production of diosgenin from yellow ginger (Dioscorea zingiberensis C. H. Wright) saponins by commercial cellulase

Liu

Huang

Sun

et al. 2009

World J Microbiol Biotechnol

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“…It is essential to mention the conditions here under which pH stability studies have been done. The temperature optima of a-l-rhamnosidases reported in the literature (Feng et al, 2007;Miake et al, 2000;Orrillo et al, 2007;Puri et al, 2000;Yu et al, 2002;Koseki et al, 2008) are in the range of 40-70°C. 1 (b) at pH 7.0 is not owing to loss of activity.…”

Section: Enzyme Characteristicsmentioning

confidence: 98%

Purification and functional characterisation of an α‐l‐rhamnosidase from Penicillium citrinum MTCC‐3565

Yadav¹,

Yadav

Yadava

et al. 2012

Int J of Food Sci Tech

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An extracellular a-l-rhamnosidase from Penicillium citrinum MTCC-3565 has purified to homogeneity from its culture filtrate using ethanol precipitation and cation-exchange chromatography on carboxymethyl cellulose. The purified enzyme gave a single protein band corresponding to molecular mass of 45.0 kDa in SDS-PAGE analysis showing the purity of the enzyme preparation. The native PAGE analysis showed the monomeric nature of the purified enzyme. Using p-nitrophenyl a-l-rhamnopyranoside as substrate, K m and V max values of the enzyme were 0.30 mm and 27.0 lm min mg )1 , respectively. The k cat value was 20.1 s giving k cat ⁄ K m value of 67.0 mm s )1 for the same substrate. The pH and temperature optima of the enzyme were 8.5 and 50°C, respectively. The activation energy for the thermal denaturation of the enzyme was 29.9 KJ mol )1 . The a-l-rhamnosidase was able to hydrolyse naringin, rutin and hesperidin and liberated l-rhamnose, indicating that the purified enzyme can be used for the preparation of a-l-rhamnose and pharmaceutically important compounds by derhamnosylation of natural glycosides containing terminal a-lrhamnose. The a-l-rhamnosidase was active at the level of ethanol concentration present in wine, indicating that it can be used for improving wine aroma.

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“…4): Dioscin ( 1 ) [9, 13, 33–47], gracillin ( 2 ) [9, 13, 33–36, 40, 42, 45–48], trillin ( 3 ) [9, 14, 36, 42, 44, 45, 49, 50], diosgenin-3- O -[ α - l -rhamnopyranosyl-(1 → 3)- α - l -rhamnopyranosyl-(1 → 4)- α - l -rhamnopyranosyl-(1 → 4)]- β - d -glucopyranoside ( 4 ) [9, 25, 45], diosgenin-3- O -{ α - l -rhamnopyranosyl-(1 → 2)-[ α - l -rhamnopyranosyl-(1 → 3)]}- β - d -glucopyranoside ( 5 ) [25, 48, 51], diosgenin ( 6 ) [9, 36, 38, 42, 45–47, 49, 52], asperin ( 7 ) [9, 13, 45], 25-Δ-spirosta-3,5-diene ( 8 ) [9, 13, 14, 45–47, 53, 54], piscidic acid ( 9 ) [9, 13, 45–47, 55], 3 β ,26-glycol-25( R )-Δ5,20(22)-diene-furostanol-26- O - β - d -glucopyranoside ( 10 ) [40, 45, 56], progenin II ( 11 ) [9, 48, 52], prosapogenin A of dioscin ( 12 , progenin III) [9, 48], pseudo-protodioscin ( 13 ) [9, 39, 40], protodioscin ( 14 ) [9, 36, 39, 40, 42, 48, 52], protogracillin ( 15 ) [9, 36, 40, 42, 45, 48], kikuba-saponin ( 16 ) [9], smilagenone ( 17 ) [45], and epistephanine ( 18 ) [45, 46]. In addition, allantoin, sterols, flavones, resins, polysaccharides and starch have also been identified as constituents in the roots of D. nipp...…”

Section: Phytochemistrymentioning

confidence: 99%

Dioscorea nipponica Makino: a systematic review on its ethnobotany, phytochemical and pharmacological profiles

Ou-yang

Jiang

Zhu

et al. 2018

Chemistry Central Journal

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Dioscorea nipponica Makino is a perennial twining herbs belonging to the family Dioscoreaceae, which is mainly distributed in the northeastern, northern, eastern and central regions of China. Traditionally, the rhizome of this herb has been commonly used by Miao and Meng ethnic groups of China to treat rheumatoid arthritis, pain in the legs and lumbar area, Kashin Beck disease, bruises, sprains, chronic bronchitis, cough and asthma. Modern pharmacological studies have discovered that this herb possesses anti-tumor, anti-inflammatory, anti-diuretic, analgesic, anti-tussive, panting-calming and phlegm-dispelling activities, along with enhancing immune function and improving cardiovascular health. In recent years, both fat-soluble and water-soluble steroidal saponins were isolated from the rhizomes of D. nipponica using silica gel column chromatography, thin layer chromatography and high performance liquid chromatography methods. Saponin and sapogenins are mainly responsible for most of the pharmacological effects of this plant. Further, the chemical components of the aboveground parts contain more than 10 kinds of phenanthrene derivatives. The present review summarizes the knowledge concerning the geographical distribution, chemical composition, pharmacological effects, toxicology studies and clinical applications of D. nipponica.

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The substrate specificity of a glucoamylase with steroidal saponin-rhamnosidase activity from Curvularia lunata

Cited by 33 publications

References 18 publications

Production of diosgenin from yellow ginger (Dioscorea zingiberensis C. H. Wright) saponins by commercial cellulase

Production of diosgenin from yellow ginger (Dioscorea zingiberensis C. H. Wright) saponins by commercial cellulase

Purification and functional characterisation of an α‐l‐rhamnosidase from Penicillium citrinum MTCC‐3565

Dioscorea nipponica Makino: a systematic review on its ethnobotany, phytochemical and pharmacological profiles

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