The success in the short-distance communication for mating does not depend on chemical signals in the crustacean decapod Procambarus clarkii (Girard, 1852)

Abstract

Pheromone-driven sex recognition has been widely documented in crayfish and a great deal of evidence supports the involvement of pheromones in their mating behaviour. This study investigates whether sexual interaction and mating success in the red swamp crayfish Procambarus clarkii are dependent on short-distance chemical communication between sexes, mediated by urine-borne pheromones. We compared the mating behaviour of intact animals that could release urine to chemically communicate in a natural way with that of urine-blocked animal pairs, for which chemical communication was precluded. Our results show that urine-borne pheromones are not critical for the reproductive success of P. clarkii, at least over the short-range distance (<1 m) considered in this study, during which the animals were confined in a restricted tank, facing one each other, and thus able to promptly mate. Under these experimental conditions, a lack of urine release neither precluded the occurrence, nor affected the duration of the different phases of mating behaviour. We conclude that short-distance chemical communication in P. clarkii is not a prerequisite for mating, and suggest that it could be affected by alternative sensory modalities, likely vision and/or acoustic signalling.

A method for selective stimulation of leg chemoreceptors in whole crustaceans

“…Chemodynamic or hydrodynamic sensory-motor integration has been comprehensively studied using decapod crustaceans, including lobsters and crayfish, as excellent models (Mellon, 2012;Schmidt and Mellon, 2011). These organisms rely on waterborne chemical cues to produce appropriate behavioural responses, such as social communication, orientation, predator avoidance, sex recognition and localisation of suitable habitats and food resources in their environment (Hartman and Hartman, 1977;Schmidt and Mellon, 2011;Thiel and Breithaupt, 2011;Peddio et al, 2019). Stimulus detection is mediated by a large number of peripheral chemoreceptor neurons (CRNs) grouped within a vast array of cuticular sensory hairs called sensilla.…”