The succinylome of Pinctada fucata martensii implicates lysine succinylation in the allograft-induced stress response

Zhang, Meizhen; Lu, Jinzhao; Liang, Haiying; Zhang, Bin; Liang, Bidan; Zou, Hexin

doi:10.1016/j.fsi.2022.07.009

Cited by 3 publications

(3 citation statements)

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“…Post LPS and PolyI:C infection, the expression levels of PmTRAF3 and PmTRAF6 increased sharply at 12 h, whereas PmTRAF2 at 24 h, which may indicate that PmTRAF3 and PmTRAF6 were more sensitive to LPS and PolyI: C model pathogen invasion than PmTRAF2. However, PmTRAF2 was responsive to LPS challenge in the gill tissue of P. f. martensii, with expression significantly up-regulated at 6 h and 12 h relative to the control group, which may be caused by the ability of gills to recognize and defend against LPS model pathogens rapidly (Wu et al, 2020;Zhang et al, 2022). Our results speculate that PmTRAFs (PmTRAF2, PmTRAF3,…”

Section: Discussionmentioning

confidence: 73%

“…The oyster Pinctada fucata martensii, a type of seawater pearl oyster, relies primarily on innate immunity to defend against invading pathogens (Zhang et al, 2022). In previous studies, the TRAF3, TRAF6, and TRAF7 genes from the pearl oyster were identified (Huang et al, 2012;Jiao et al, 2014;Lei et al, 2016); Huang et al and Jiao et al have observed that TRAF3 and TRAF6 are involved in the innate immune response to V. alginolyticus and LPS stimulation, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and function of two tumor necrosis factor receptor-associated factors genes (TRAF2 and TRAF4) from Pinctada fucata martensii

et al. 2023

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Tumor necrosis factor receptor-associated factors (TRAFs) have been studied in a few mollusks and participate in various biological processes, like apoptosis, immune response, stress, and inflammatory response. However, TRAFs’ function and mechanism of pearl oysters (Pinctada fucata martensii) are still unclear. In this study, we identified novel PmTRAF2 and PmTRAF4 from P. f. martensii. The deduced PmTRAF2 and PmTRAF4 proteins contain TRAF-type zinc finger domain and MATH domain, while PmTRAF4 lacks a RING finger domain. Multiple sequence alignment revealed that PmTRAF2 and PmTRAF4 had high homology with the ortholog of other species. Phylogenic analysis indicated that PmTRAF4 was closer to the homolog protein of Mytilus edulis and Mytilus galloprovincialis, and PmTRAF2 was closer to Crassostrea gigas TRAF2. The real-time quantitative PCR (qPCR) analysis revealed that PmTRAF2 and PmTRAF4 were expressed in all six tissues, and both of them were significantly expressed in hepatopancreas and gill (p < 0.01). Under lipopolysaccharide (LPS) stimulation, polyinosinic acid (PolyI:C) stimulation, and nucleus insertion surgery, the transcripts of PmTRAF2, PmTRAF3, PmTRAF4 and PmTRAF6 in hepatopancreas were markedly changed at corresponding time points. These results have indicated that these genes may play a role in P. f. martensii innate immunity. Yeast two-hybrid assays show that PmTRAF2 interacts with PmTRAF6 but not PmTRAF3, potentially affecting downstream immune signaling pathways. Our findings provide new perspectives for further investigation of TRAFs’ immune mechanisms in bivalves.

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Section: Discussionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Molecular cloning and function of two tumor necrosis factor receptor-associated factors genes (TRAF2 and TRAF4) from Pinctada fucata martensii

et al. 2023

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“…The pearl oyster Pinctada fucata martensii is an economically important bivalve that is farmed for marine pearl production globally, with most Chinese marine pearls produced from this species (Zhang et al, 2022). The production of seawater pearls in China is primarily based on artificial cultivation.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis reveals immune-related genes involved in allograft and xenograft transplantation in Pinctada fucata

Zheng,

Wang,

Guo

et al. 2024

Front. Mar. Sci.

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BackgroundThe marine pearl culture industry is a key industry in the Beibu Gulf of China that achieves large-scale pearl production by artificial nucleus insertion in pearls. High-quality pearls can produced by xenotransplantation, but allotransplantation or xenotransplantation can lead to various immune responses, resulting in nucleus rejection or even the recipient shell death and thereby causing significant losses in pearl production.MethodsFew studies have investigated the immune defenses of oysters related to allografts and xenografts. In this study, transcriptomic comparisons of allograft and xenograft Pinctada fucata haemocytes were conducted to identify genes associated with immune responses.ResultsA total of 33.11 Gbp of clean reads were generated from five P. fucata haemocytes. De-novo assembly of quality-filtered reads generated a total of 26,526 unigenes, with 22,002 known genes and 4,524 predicted novel genes. In addition, 34,904 novel transcripts were detected, with 15,620 novel alternative splicing isoforms of known protein coding genes and 4,605 belonging to novel protein coding genes, with the remaining 14,679 comprising long non-coding RNA transcripts. Functional enrichment analysis of immune-related differentially expressed genes (DEGs) using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases revealed 36–44 significantly enriched GO terms and 34 significantly enriched KEGG pathways. Ten DEGs were subjected to validation of expression levels using RT-q PCR analysis, revealing generally consistent values as the high-throughput sequencing data.ConclusionOyster haemocytes were comprehensively evaluated in this study using transcriptomic comparisons and with a focus on immune-related functional genes and pathways. The results revealed numerous DEGs related to immune function that can serve as the basis for subsequent immune response analysis of allotransplantation and xenotransplantation.

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Phosphoproteomics changes due to allograft-induced stress responses of Pinctada fucata martensii

Chen,

Liang,

et al. 2024

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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The succinylome of Pinctada fucata martensii implicates lysine succinylation in the allograft-induced stress response

Cited by 3 publications

References 43 publications

Molecular cloning and function of two tumor necrosis factor receptor-associated factors genes (TRAF2 and TRAF4) from Pinctada fucata martensii

Molecular cloning and function of two tumor necrosis factor receptor-associated factors genes (TRAF2 and TRAF4) from Pinctada fucata martensii

Comparative transcriptome analysis reveals immune-related genes involved in allograft and xenograft transplantation in Pinctada fucata

Phosphoproteomics changes due to allograft-induced stress responses of Pinctada fucata martensii

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