The Sugar Transporter family in wheat (Triticum aestivum. L): genome-wide identification, classification, and expression profiling during stress in seedlings

Abstract

The sugar transporter protein (STP) plays a crucial role in regulating plant growth and stress tolerance. We performed genome-wide identification and expression analysis of the STP gene family to investigate the STPSs’ potential roles in the growth of wheat seedlings under stress. Here, a total of 81 TaSTP genes containing the Sugar_tr conserved motif were identified within the wheat genome. Bioinformatic studies including phylogenetic tree, chromosome position, and tandem repeat were performed to analyze the identified genes. The 81 TaSTP genes can be classified into five main groups according to their structural and phylogenetic features, with several subgroups, which were located separately on chromosomes A, B, and D. Moreover, six gene clusters were formed with more than three genes each. The results of three comparative syntenic maps of wheat associated with three representative species suggested that STP genes have strong relationships in monocots. qRT-PCR analysis confirmed that most TaSTP genes displayed different expression profiles after seedlings were subjected to six days of different stress (10% PEG6000, 150 mM NaCl, and their combination, respectively), suggesting that these genes may be involved in regulating plant growth and stress tolerance. In conclusion, 81 TaSTP genes were identified and their expressions changed under stress, indicating TaSTP’s potential roles in wheat growth monosaccharide distribution is regulated.

Genome-wide identification of PIP5K in wheat and its relationship with anther male sterility induced by high temperature

“…Specific primers were designed using primer premier 5.0 software for the qRT-PCR; the primer sequence details are provided in Table S 1 . Detailed information on the qRT-PCR protocol is described in a previous study [ 40 ].…”

Genome‐wide transcriptional responses to water deficit during seed development in Pisum sativum, focusing on sugar transport and metabolism

“…Moreover, numerous genes of the ESL and STP clades were found regulated in all organs by WD (Figure 6). Both ESL and STP candidates are well characterized for their importance in abiotic stress response, including water deficit (Yamada et al, 2011; Liu et al, 2021; Slawinski et al, 2021). These candidate genes may represent interesting targets for improving the efficiency with which plants take up and use water and nutrients (Schroeder et al, 2013; Varshney et al, 2023).…”

“…Genes encoding polyol and inositol transporter (PLT and INT; Figure 6) were also found up-regulated by WD. This transcriptional regulation could help maintain the osmotic pressure in seeds subjected to water stress (Li et al, 2021).…”

Fruit shape loci sun, ovate, fs8.1 and their interactions affect seed size and shape in tomato

“…Similar to what happened in sun NIL, expression of SWEET and VI2 was also down-regulated in ovate NIL ( Figure 5B ). Furthermore, expression of STP1 , which belongs to the monosaccharide transporter ( MST ) gene family and plays a key role in maintaining source/sink characteristics ( Liu et al., 2021 ), was also repressed ( Figure 5B ), suggesting ovate may have a stronger impact on the source-sink relationship of seed than sun . Different from what is mentioned above, fs8.1 increased the expression of CWI1 ( Figure 5B ).…”