The suitability of high throughput automated patch clamp for physiological applications

Abstract

support-information-section).

Basic Research Approaches to Evaluate Cardiac Arrhythmia in Heart Failure and Beyond

“…This is exacerbated when patching iPSC-CM which are often heterogeneous, varying in shape, size and electrophysiological phenotype. Many of these limitations are being overcome using easy-to-handle automated patch clamp systems, which can improve throughput and standardisation and are comprehensively described in Suk et al (2019), Obergrussberger et al (2021), and Bell and Fermini (2021).…”

Biophysical characterization of light-gated ion channels using planar automated patch clamp

“…The SyncroPatch 384i offers unprecedented high throughput close to that of non-electrophysiological screening techniques such as ion-flux measurements and fluorescence assays, with the added benefit of real-time monitoring of channel activity. This instrument and its earlier modifications have already been used to probe voltage- and ligand-gated ion channels in a variety of cell types ( Brinkwirth et al, 2020 ; Potet et al, 2020 ; Obergrussberger et al, 2022 ).…”

Ionotropic glutamate receptors: structure, function and dysfunction

“…Reviews from this event appear in this issue of The Journal of Physiology. The five review articles (Crawley et al 2022;Frydenvang et al 2022;Geoffroy et al 2022;Pampaloni & Plested, 2022;von Engelhardt, 2022) and one Techniques for Physiology paper (Obergrussberger et al 2022) in this issue are a lasting legacy of the day's presentations and provide us with an excellent overview of aspects of contemporary ionotropic glutamate receptor research.…”

“…Indeed the review concludes by highlighting neuropsychiatric disorders and neurodevelopmental and neurodegenerative diseases where GRIN3A mutations may contribute to disease aetiology or progression. The final article to appear in this issue and associated with the iGluR online symposium is a Techniques for Physiology paper by Obergrussberger and colleagues highlighting the advances and applications of high throughput methods for automated electrophysiological studies and specifically patch-clamp recording (Obergrussberger et al 2022). As both of us can testify, conventional patch-clamp recording methods are labour-intensive, often repetitive and frequently frustratingthat being said, the child-like enthusiasm and delight with which a near-perfect recording is met is the reward for many unproductive hours sitting at an electrophysiological recording set-up.…”

“…2022; Pampaloni & Plested, 2022; von Engelhardt, 2022) and one Techniques for Physiology paper (Obergrussberger et al . 2022) in this issue are a lasting legacy of the day's presentations and provide us with an excellent overview of aspects of contemporary ionotropic glutamate receptor research.…”