The suitability of yellow fever and Japanese encephalitis vaccines for immunization against West Nile virus

Yamshchikov, Galina V.; Borisevich, Victoria; Kwok, Chun Wai; Nistler, Ryan; Kohlmeier, Jake; Seregin, Alexey; Чапоргина, Е. А.; Benedict, Stephen H.; Yamshchikov, Vladimir

doi:10.1016/j.vaccine.2005.04.036

Cited by 47 publications

(28 citation statements)

References 22 publications

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“…4). The existence of cross protection between heterologous but closely related members of the Flaviviridae family has been reported (62)(63)(64)(65)(66). Neutralization assay showed that the titer of neutralization antibody against JEV was relatively low and may not account for the in vivo protection.…”

Section: Discussionmentioning

confidence: 99%

A Chimeric Dengue Virus Vaccine using Japanese Encephalitis Virus Vaccine Strain SA14-14-2 as Backbone Is Immunogenic and Protective against Either Parental Virus in Mice and Nonhuman Primates

Deng

Yang

et al. 2013

J Virol

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The development of a safe and efficient dengue vaccine represents a global challenge in public health. Chimeric dengue viruses (DENV) based on an attenuated flavivirus have been well developed as vaccine candidates by using reverse genetics. In this study, based on the full-length infectious cDNA clone of the well-known Japanese encephalitis virus live vaccine strain SA14-14-2 as a backbone, a novel chimeric dengue virus (named ChinDENV) was rationally designed and constructed by replacement with the premembrane and envelope genes of dengue 2 virus. The recovered chimeric virus showed growth and plaque properties similar to those of the parental DENV in mammalian and mosquito cells. ChinDENV was highly attenuated in mice, and no viremia was induced in rhesus monkeys upon subcutaneous inoculation. ChinDENV retained its genetic stability and attenuation phenotype after serial 15 passages in cultured cells. A single immunization with various doses of ChinDENV elicited strong neutralizing antibodies in a dose-dependent manner. When vaccinated monkeys were challenged with wild-type DENV, all animals except one that received the lower dose were protected against the development of viremia. Furthermore, immunization with Chin-DENV conferred efficient cross protection against lethal JEV challenge in mice in association with robust cellular immunity induced by the replicating nonstructural proteins. Taken together, the results of this preclinical study well demonstrate the great potential of ChinDENV for further development as a dengue vaccine candidate, and this kind of chimeric flavivirus based on JE vaccine virus represents a powerful tool to deliver foreign antigens.

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Section: Discussionmentioning

confidence: 99%

A Chimeric Dengue Virus Vaccine using Japanese Encephalitis Virus Vaccine Strain SA14-14-2 as Backbone Is Immunogenic and Protective against Either Parental Virus in Mice and Nonhuman Primates

Deng

Yang

et al. 2013

J Virol

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“…While other studies have looked at cross-reactivity with a limited number of tests and single-time-point samples (17,46), our sequential infection studies provided a complete set of serum samples collected every 3 days for over 2 months from animals that were known to be free of any previous flavivirus exposure. Furthermore, our study was geographically relevant for our objective and incorporated all the widely available and used equine diagnostic assays.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Widely Used Diagnostic Tests To Detect West Nile Virus Infections in Horses Previously Infected with St. Louis Encephalitis Virus or Dengue Virus Type 2

Ledermann

Loroño-Pino

Ellis

et al. 2011

Clin Vaccine Immunol

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Primary West Nile virus (WNV) infections can be diagnosed using a number of tests that detect infectious particles, nucleic acid, and specific IgM and/or IgG antibodies. However, serological identification of the infecting agent in secondary or subsequent flavivirus infections is problematic due to the extensive cross-reactivity of flavivirus antibodies. This is particularly difficult in the tropical Americas where multiple flaviviruses cocirculate. A study of sequential flavivirus infection in horses was undertaken using three medically important flaviviruses and five widely utilized diagnostic assays to determine if WNV infection in horses that had a previous St. Louis encephalitis virus (SLEV) or dengue virus type 2 (DENV-2) infection could be diagnosed. Following the primary inoculation, 25% (3/12) and 75% (3/4) of the horses mounted antibody responses against SLEV and DENV-2, respectively. Eighty-eight percent of horses subsequently inoculated with WNV had a WNV-specific antibody response that could be detected with one of these assays. The plaque reduction neutralization test (PRNT) was sensitive in detection but lacked specificity, especially following repeated flavivirus exposure. The WNV-specific IgM enzyme-linked immunosorbent assay (IgM ELISA) was able to detect an IgM antibody response and was not crossreactive in a primary SLEV or DENV response. The WNV-specific blocking ELISA was specific, showing positives only following a WNV injection. Of great importance, we demonstrated that timing of sample collection and the need for multiple samples are important, as the infecting etiology could be misdiagnosed if only a single sample is tested.

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“…In recent years it has been described the successful construction of full-length cDNA clones for several members of the flavivirus genus including YFV, dengue virus, West Nile virus and Kunjin virus (KUNV), among others (Bredenbeek et al 2003, Imoto and Konish 2007, Khromykh and Westaway 1994, Polo et al 1997, Puri et al 2000, Sumiyoshi et al 1992, Yamshchikov et al 2005.…”

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confidence: 99%

Construction of yellow fever virus subgenomic replicons by yeast-based homologous recombination cloning technique

Queiroz

Silva

Santos

et al. 2013

An. Acad. Bras. Ciênc.

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RNA replicon derived from Flavivirus genome is a valuable tool for studying viral replication independent of virion assembly and maturation, besides being a great potencial for heterologous gene expression. In this study we described the construction of subgenomic replicons of yellow fever virus by yeast-based homologous recombination technique. The plasmid containing the yellow fever 17D strain replicon (pBSCrepYFV-17D), previously characterized, was handled to heterologous expression of the green fluorescent protein (repYFV-17D-GFP) and firefly luciferase (repYFV-17D-Luc) reporter genes. Both replicons were constructed by homologous recombination between the linearized vector pBSC-repYFV-17D and the PCR product containing homologous 25 nucleotides ends incorporated into PCR primers. The genomic organization of these constructs is similar to repYFV-17D, but with insertion of the reporter gene between the remaining 63 N-terminal nucleotides of the capsid protein and 72 C-terminal nucleotides of the E protein. The replicons repYFV-17D-GFP and repYFV-17D-Luc showed efficient replication and expression of the reporter genes. The yeast-based homologous recombination technique used in this study proved to be applicable for manipulation of the yellow fever virus genome in order to construct subgenomic replicons.

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The suitability of yellow fever and Japanese encephalitis vaccines for immunization against West Nile virus

Cited by 47 publications

References 22 publications

A Chimeric Dengue Virus Vaccine using Japanese Encephalitis Virus Vaccine Strain SA14-14-2 as Backbone Is Immunogenic and Protective against Either Parental Virus in Mice and Nonhuman Primates

A Chimeric Dengue Virus Vaccine using Japanese Encephalitis Virus Vaccine Strain SA14-14-2 as Backbone Is Immunogenic and Protective against Either Parental Virus in Mice and Nonhuman Primates

Evaluation of Widely Used Diagnostic Tests To Detect West Nile Virus Infections in Horses Previously Infected with St. Louis Encephalitis Virus or Dengue Virus Type 2

Construction of yellow fever virus subgenomic replicons by yeast-based homologous recombination cloning technique

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