The (Sun)Light and Dark of 25-Hydroxyvitamin D Testing

French, Deborah

doi:10.1373/jalm.2017.023051

Cited by 4 publications

(1 citation statement)

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“…Otro aspecto para la interpretación de resultados a considerar es la variedad de ensayos disponibles para medir VitD que no siempre son comparables, a pesar de los esfuerzos que se han realizado para estandarizarlos. En una comparación de distintos métodos para medir una muestra de Control de Calidad Externo con una concentración de 19 ng/mL, el promedio varió entre 15 y 22 ng/mL, diferencias que pueden ser aún mayores cuando se analizan muestras de pacientes 39 . En nuestro estudio las muestras fueron analizadas por espectrometría de masa, que es considerado el método de referencia.…”

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Variación estacional de 25-hidroxi-vitamina D3, hormona paratiroidea y fosfatasa alcalina en niños escolares

et al. 2020

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El principal rol de la vitamina D es la regulación del metabolismo del calcio, cuya principal fuente es la vitamina D3 que se obtiene principalmente por la acción de la luz ultravioleta (UV) en la piel.Objetivo: Evaluar las diferencias estacionales en las concentraciones de 25-hidroxi-vitamina D3 (25OHVitD3), hormona paratiroidea (PTH), fosfatasa alcalina (FA) y calcio en niños en edad escolar.Sujetos y Método: Se midieron las concentraciones de 25OHVitD3, PTH, FA y calcio en niños de 5 a 8 años, sin suplementación de Vitamina D, reclutados en Santiago de Chile (latitud -33.4372) en distintas estaciones del año. El estatus de VitD fue definido como suficiente con concentraciones de 25OHVitD3 > 20 ng/mL (50 nmol/L), insuficiente 12-20 ng/mL (30-50 nmol/L) y deficiente < 12 ng/ mL (30 nmol/L) en base a las recomendaciones del grupo de expertos del “Consenso Global para la Prevención y Manejo del Raquitismo Nutricional”.Resultados: En total participaron 133 niños (89 prematuros menor o igual a 32 semanas), 41 durante el verano, 28 en otoño, 35 en invierno y 29 en primavera. La diferencia de las medias entre el verano y el invierno fue de 9,6 ng/mL para 25OHVitD3 (p < 0,0001), -11,1 pg/mL para PTH (p < 0,0001) y -47,5 UI/mL para FA (p = 0,01). En las concentraciones de calcio no se observaron diferencias. El 97,6% de los sujetos fueron clasificados con estatus de suficiencia (> 20 ng/mL) en verano, lo que disminuyó significativamente en invierno (54,3%, p < 0,0001).Conclusiones: Las concentraciones de 25OHVitD3 disminuyeron en aproximadamente la mitad de los niños durante el invierno, lo que se vio acompañado de un aumento de la PTH y FA, asociado a concentraciones normales de calcio. De acuerdo a nuestros resultados, la suplementación con VitD en niños podría ser necesaria durante otoño e invierno

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Section: Discussionunclassified

Variación estacional de 25-hidroxi-vitamina D3, hormona paratiroidea y fosfatasa alcalina en niños escolares

et al. 2020

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Serum 25-Hydroxyvitamin D Concentrations from Patient Groups at High Risk for Deficiency

Ghosh

Ong

et al. 2019

The Journal of Applied Laboratory Medicine

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Background Deficiency of 25-hydroxyvitamin D in serum is endemic in the general population, and testing for this hormone is useful in accessing a patient's overall health and well-being. Methods We obtained blood from 216 hospitalized patients and outpatients divided into 4 groups thought to be at high risk of 25-hydroxyvitamin D deficiency: homeless, recreational drug abusers, psychiatric patients with limited access to the outdoors, and those infected with HIV. The 25-hydroxyvitamin D concentrations from these patients were determined with 2 different methodologies (immunoassay and mass spectrometry) and compared against 25-hydroxyvitamin D concentrations in apparently healthy controls. We hypothesized that these groups may be at higher risk for vitamin D deficiency because of poor nutrition, inadequate housing, restricted access to outdoors, or the presence of chronic disease. Results For each of the patient groups including healthy controls, the median concentration of 25-hydroxyvitamin D was below 30 ng/mL, indicating deficiency. Comparisons between the healthy controls and the other groups were not statistically significant with either methodology, except for the homeless patients in whom a higher number of individuals had 25-hydroxyvitamin D concentrations below 20 ng/mL. Results between the 2 testing platforms demonstrated that only 52% of the specimens analyzed by immunoassay agreed within ±10% of the LC-MS/MS results, with an overall correlation coefficient to 0.920. The degree of concordance for deficiency with 2 published cutoffs of 20 and 30 ng/mL was 91% and 91%, respectively. Conclusions Vitamin D deficiency is a common finding in all the populations studied. The Lumipulse® G vitamin D immunoassay is an alternative for detecting vitamin D deficiency.

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Evaluation of a New Generation Automated Assay for 25-Hydroxy Vitamin D Based on Competitive Protein Binding

Asif

Groboske

Leung

et al. 2019

The Journal of Applied Laboratory Medicine

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Background The interest for vitamin D has exponentially increased testing demand for 25-hydroxy vitamin D [25(OH)D]. Consequently, many laboratories are switching from LC-MS/MS methods to automated, high-throughput immunoassays. One of the major potential issues with these assays has been the lack of cross-reactivity with 25(OH)D2. Methods We have evaluated the Roche Elecsys vitamin D total II assay for accuracy by comparing 79 patient samples with LC-MS/MS. The cross-reactivity for 25(OH)D2 was evaluated by analyzing samples with high 25(OH)D2 separately and estimating 25(OH)D2 recovery, as well as by spiking of 25(OH)D2. The assay was further evaluated for precision, linearity, sample type, and common interferences. Results There was mostly good agreement between the Elecsys and LC-MS/MS assays (Deming regression: y = 0.95x + 0.70), with an overall bias of 2.3% (−0.84 ng/mL). However, there were 6 out of 79 (7.6%) discordant samples. The Deming regression for samples with high 25(OH)D2 compared to LC-MS/MS showed similar slope and intercept (y = 0.97x − 1.1). The average recovery of 25(OH)D2 for these samples was 90%. The initial precision studies were in general agreement with the package insert, but long-term clinical use showed higher-than-claimed imprecision (11.7%–14.4% at 12 ng/mL and 6.9%–7.6% at 27 ng/mL; claimed: 7.2% and 5.0%, respectively). We observed 1 falsely high result in plasma, an issue previously addressed by Roche in a medical device correction. Conclusions The analytical performance of the Roche Vitamin D assay was acceptable, and the assay had a good cross-reactivity for 25(OH)D2.

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The (Sun)Light and Dark of 25-Hydroxyvitamin D Testing

Cited by 4 publications

References 64 publications

Variación estacional de 25-hidroxi-vitamina D3, hormona paratiroidea y fosfatasa alcalina en niños escolares

Variación estacional de 25-hidroxi-vitamina D3, hormona paratiroidea y fosfatasa alcalina en niños escolares

Serum 25-Hydroxyvitamin D Concentrations from Patient Groups at High Risk for Deficiency

Evaluation of a New Generation Automated Assay for 25-Hydroxy Vitamin D Based on Competitive Protein Binding

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