The Suppressive Effect of Butyrate and Bromopyruvate on Inflammatory Cytokine Production and Short Chain Fatty Acid Receptor Expression by Blood Mononuclear Cells in Patients with Behçet's Disease

Yun, Su Jin; Kim, Kyongmin; Lee, Eun‐So; Park, Sun

doi:10.5021/ad.2018.30.5.566

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…We used much lower amounts of BrPA (20, 80, and 200 μM), as we wanted to examine the therapeutic potential of BrPA against Candida infection at the concentration that minimized human cell death. Our previous study found slightly decreased cell survival of peripheral blood mononuclear cells at 80 μM of BrPA 40. In this study, we demonstrated that both 80 and 200 μM of BrPA synergistically enhanced cell death in C. albicans (Figure 10), thus revealing the potential of the combination of AgNPs and BrPA as an antifungal agent against C. albicans .…”

Section: Discussionsupporting

confidence: 66%

Silver nanoparticles induce reactive oxygen species-mediated cell cycle delay and synergistic cytotoxicity with 3-bromopyruvate in Candida albicans, but not in Saccharomyces cerevisiae

Lee

Yun

et al. 2019

IJN

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Background: Silver nanoparticles (AgNPs) inhibit the proliferation of various fungi; however, their mechanisms of action remain poorly understood. To better understand the inhibitory mechanisms, we focused on the early events elicited by 5 nm AgNPs in pathogenic Candida albicans and non-pathogenic Saccharomyces cerevisiae. Methods: The effect of 5 nm and 100 nm AgNPs on fungus cell proliferation was analyzed by growth kinetics monitoring and spot assay. We examined cell cycle progression, reactive oxygen species (ROS) production, and cell death using flow cytometry. Glucose uptake was assessed using tritium-labeled 2-deoxyglucose. Results: The growth of both C. albicans and S. cerevisiae was suppressed by treatment with 5 nm AgNPs but not with 100 nm AgNPs. In addition, 5 nm AgNPs induced cell cycle arrest and a reduction in glucose uptake in both fungi after 30 minutes of culture in a dose-dependent manner ( P <0.05). However, in C. albicans only, an increase in ROS production was detected after exposure to 5 nm AgNPs. Concordantly, an ROS scavenger blocked the effect of 5 nm AgNPs on the cell cycle and glucose uptake in C. albicans only. Furthermore, the growth-inhibition effect of 5 nm AgNPs was not greater in S. cerevisiae mutant strains deficient in oxidative stress response genes than it was in wild type. Finally, 5 nm AgNPs together with a glycolysis inhibitor, 3-bromopyruvate, synergistically enhanced cell death in C. albicans ( P <0.05) but not in S. cerevisiae . Conclusion: AgNPs exhibit antifungal activity in a manner that may or may not be ROS dependent, according to the fungal species. The combination of AgNPs with 3-bromopyruvate may be more useful against infection with C. albicans .

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Section: Discussionsupporting

confidence: 66%

Silver nanoparticles induce reactive oxygen species-mediated cell cycle delay and synergistic cytotoxicity with 3-bromopyruvate in Candida albicans, but not in Saccharomyces cerevisiae

Lee

Yun

et al. 2019

IJN

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“…Therefore, a diet change might not have been the primary factor altering the abundance of B. uniformis in the gut microbiota during BD disease activity shift. Given our previous observation that SCFA negatively affects the production of inflammatory cytokines in patients with BD [ 46 ], we speculated an alteration in the abundance of SCFA producers during BD disease activity shift. Our findings do not concord with this speculation, but rather show an alteration in B. uniformis abundance, suggesting a possible role of the gut microbiome in BD pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

Alteration of the Fecal but Not Salivary Microbiome in Patients with Behçet’s Disease According to Disease Activity Shift

Kim

Park

et al. 2021

Microorganisms

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The human microbiome plays an important role in various diseases, including Behçet’s disease (BD). However, the effects of disease activity and covariates influencing the microbial composition have not yet been investigated. Therefore, we investigated the fecal and salivary microbiomes of BD patients compared to those of recurrent aphthous ulcer (RAU) patients, as well as dietary habit-matched healthy controls (HCs) selected from immediate family members using 16S rRNA gene sequencing. The fecal microbiome alpha diversity of BD patients was not different from that of their matched HCs, although it was higher than that of unrelated HCs and decreased in BD patients with disease activity. A tendency toward clustering in the beta diversity of the fecal microbiome was observed between the active BD patients and their matched HCs. Active BD patients had a significantly higher abundance of fecal Bacteroides uniformis than their matched HCs and patients with the disease in an inactive state (p = 0.038). The abundance of salivary Rothia mucilaginosa group was higher in BD patients than in RAUs patients. BD patients with uveitis had different abundances of various taxa, compared to those without uveitis. Our results showed an association of fecal microbiome composition with BD disease activity and symptoms, suggesting the possible role of the gut microbiome in BD pathogenesis.

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“…Patients with Crohn’s disease ( 29 , 30 ), rheumatoid arthritis (RA) ( 31 ), and ulcerative colitis ( 32 ) have significantly reduced amounts of E. rectale , and these patients have lower butyrate concentrations in their feces than healthy individuals. Butyrate treatment downregulated IL-6 in peripheral blood mononuclear cell cultures of patients with BD ( 33 ) and the downregulation of IL-6 was associated with BD symptom improvement in mice ( 34 ). Metagenomics studies have shown that the gut microbiota is associated with human diseases ( 35 ).…”

Section: Introductionmentioning

confidence: 99%

Eubacterium rectale Attenuates HSV-1 Induced Systemic Inflammation in Mice by Inhibiting CD83

et al. 2021

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The purpose of this study was to determine whether administration of the microorganism Eubacterium rectale (E. rectale) could regulate dendritic cell (DC) activation and systemic inflammation in herpes simplex virus type 1-induced Behçet’s disease (BD). E. rectale, butyrate-producing bacteria, was administered to BD mice. Peripheral blood leukocytes (PBL) and lymph node cells were isolated and analyzed by flow cytometry. 16S rRNA metagenomic analysis was performed in the feces of mice to determine the differences in the composition of the microbial population between normal and BD mice. Serum cytokine levels were measured by enzyme-linked immunosorbent assay. The frequency of DC activation marker CD83 positive cells was significantly increased in PBL of BD mice. Frequencies of CD83+ cells were also significantly increased in patients with active BD. 16S rRNA metagenomic analysis revealed different gut microbiota composition between normal and BD mice. The administration of E. rectale to BD mice reduced the frequency of CD83+ cells and significantly increased the frequency of NK1.1+ cells with the improvement of symptoms. The co-administration of colchicine and E. rectale also significantly reduced the frequency of CD83+ cells. Differences in gut microbiota were observed between normal mice and BD mice, and the administration of E. rectale downregulated the frequency of CD83, which was associated with BD deterioration. These data indicate that E. rectale could be a new therapeutic adjuvant for BD management.

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The Suppressive Effect of Butyrate and Bromopyruvate on Inflammatory Cytokine Production and Short Chain Fatty Acid Receptor Expression by Blood Mononuclear Cells in Patients with Behçet's Disease

Cited by 4 publications

References 19 publications

<p>Silver nanoparticles induce reactive oxygen species-mediated cell cycle delay and synergistic cytotoxicity with 3-bromopyruvate in <em>Candida</em> <em>albicans</em>, but not in <em>Saccharomyces</em> <em>cerevisiae</em></p>

<p>Silver nanoparticles induce reactive oxygen species-mediated cell cycle delay and synergistic cytotoxicity with 3-bromopyruvate in <em>Candida</em> <em>albicans</em>, but not in <em>Saccharomyces</em> <em>cerevisiae</em></p>

Alteration of the Fecal but Not Salivary Microbiome in Patients with Behçet’s Disease According to Disease Activity Shift

Eubacterium rectale Attenuates HSV-1 Induced Systemic Inflammation in Mice by Inhibiting CD83

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