The suppressive effect of tape-stripping treatment of guinea-pig skin on the induction of contact sensitivity by intradermal injection of haptenated epidermal cells

Oka, Daisuke; Nakagawa, Shojiro; Ueki, Hiroaki

doi:10.1007/bf00409215

Cited by 6 publications

(2 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since it has been reported that excessive tapestrippings induce immuno-suppression in contact hypersensitivity [16], we determined whether the degree of contact hypersensitivity by PiCl as measured by ear swelling is also suppressed by tape-stripping under our experimental conditions. Eight tape-strippings during the sensitization phase did not reduce the intensity of the PiCl-induced contact hypersensitivity on aural skin as compared to that without tape-stripping ( Fig.…”

Section: Effects Of Barrier Disruption On the Degree Of Contact Hypermentioning

confidence: 99%

Percutaneous sensitization with allergens through barrier-disrupted skin elicits a Th2-dominant cytokine response

1998

View full text Add to dashboard Cite

We investigated whether percutaneous sensitization with different allergens through barrier-disrupted skin regulates the balance of Th1/Th2 cytokine expression. When mice were sensitized with the typical hapten picryl chloride (PiCl) by a single topical application to intact skin, there was an up-regulation in the lymph nodes (LN) of mRNA expression for the Th1 cytokines IL-2 or IFN-gamma, and for the Th2 cytokine IL-4. In contrast, sensitization with PiCl after barrier disruption of the skin down-regulated the expression of mRNA for IFN-gamma in a tape-stripping number-dependent manner without changing the expression of mRNA for IL-4. When mice were sensitized with house dust mite antigens (MA) by a single topical application to barrier-disrupted abdominal skin, there was a tape-stripping number-dependent up-regulation in the LN of mRNA expression for IL-4 but not for IL-2 or IFN-gamma. In the LN, mRNA for the IL-4-inducible immunoglobulins IgE and IgG1, but not for the IFN-gamma-inducible IgG2a, were up-regulated after sensitization with MA, while all three immunoglobulin mRNA were augmented after PiCl sensitization through intact skin. Antigenic elicitation by a topical application of PiCl in aural skin of mice sensitized through intact skin consistently increased the expression of mRNA for all three cytokines in the challenged skin, whereas elicitation in mice sensitized through barrier-disrupted skin decreased the expression of mRNA for IL-2 and IFN-gamma, but not for IL-4. Antigenic elicitation by subcutaneous injection of MA in aural skin consistently increased the expression of mRNA for IL-4, but not for IL-2 or IFN-gamma in the challenged skin. Infiltration of eosinophils in the dermis was more prominent following elicitation with MA in mice sensitized through barrier disruption than with PiCl in mice sensitized through intact skin. These findings suggest that the percutaneous entry of environmental allergens through barrier-disrupted skin is strongly associated with the induction of Th2-dominant immunological responses, as is seen in atopic dermatitis.

show abstract

Section: Effects Of Barrier Disruption On the Degree Of Contact Hypermentioning

confidence: 99%

Percutaneous sensitization with allergens through barrier-disrupted skin elicits a Th2-dominant cytokine response

1998

View full text Add to dashboard Cite

show abstract

“…Accordingly, enhanced CHS responses to dinitrofluorobenzene and picryl-chloride have been observed (Nishijima et al, 1997). In contrast, Oka et al (1985) reported reduced CHS responses to dinitrochlorobenzene on tape stripping. Impaired sensitization was initially explained by the finding that Langerhans cells are depleted by tape stripping (Streilein et al, 1982).…”

Section: Discussionmentioning

confidence: 99%

Disruption of the Epidermal Barrier Induces Regulatory T Cells via IL-33 in Mice

Bruhs

Proksch

Schwarz

et al. 2018

Journal of Investigative Dermatology

View full text Add to dashboard Cite

Disturbance of the epidermal barrier by UVR is associated with the release of antimicrobial peptides and inflammatory cytokines for the purpose of a danger response. On the other hand, UVR causes immunosuppression via regulatory T cells (Treg) that limit the inflammatory reaction. The concurrent induction of antimicrobial peptides and Treg by UVR may represent a counter-regulatory mechanism in response to barrier disruption, preventing microbial superinfection and sensitization to contact allergens, respectively, both of which cross impaired epidermis more easily. Thus, using a model of murine contact hypersensitivity we examined if disruption of the epidermal barrier only initiates similar counter-regulatory mechanisms via the generation of Treg. Sensitization through tape-stripped skin induced a weaker contact hypersensitivity response than in control mice. This was due to the induction of antigen-specific Treg, as demonstrated in adoptive transfer and depletion experiments utilizing DEREG mice. Treg induction by tape stripping was linked to the expression of the alarmin IL-33, as blockade of IL-33 exacerbated contact hypersensitivity, whereas injection of IL-33 inhibited contact hypersensitivity and induced Treg. These results demonstrate that epidermal barrier disruption, in addition to danger signals, induces regulatory events that prevent exaggerated skin inflammation and that IL-33 appears to be critically involved in this process.

show abstract

Delayed-type skin reaction to 2,4-dinitrophenylated epidermal cells in guinea pigs with contact sensitivity to 2,4-dinitrochlorobenzene

et al. 1987

View full text Add to dashboard Cite

Contact sensitivity (CS) induced by hapten has been thought to be analogous to delayed-type hypersensitivity, such as the Mantoux reaction, because of outstanding similarities between the two phenomena. It can be suggested that animals with CS respond also to intradermal injection of the conjugate of hapten and protein as well as to epicutaneous application of hapten. However, evidence against this has been reported. In the present experiments, delayed-type skin reaction (DSR) was successfully obtained in JY1 strain guinea pigs sensitized by painting the skin with 2,4-dinitrochlorobenzene using in vitro dinitrophenylated epidermal cell suspension (DNP-EC) as antigen for a delayed intradermal test. The experiment using anti-Ia alloantiserum and complement showed that the elicitation of DSR is due to the presence of Ia-positive cells (presumably Langerhans cells) among DNP-ECs. The delayed intradermal test with the conjugates such as haptenated ECs in the animals with CS is considered to be an experimentally useful way of analysing the antigen in the sensitivity.

show abstract

The suppressive effect of tape-stripping treatment of guinea-pig skin on the induction of contact sensitivity by intradermal injection of haptenated epidermal cells

Cited by 6 publications

References 21 publications

Percutaneous sensitization with allergens through barrier-disrupted skin elicits a Th2-dominant cytokine response

Percutaneous sensitization with allergens through barrier-disrupted skin elicits a Th2-dominant cytokine response

Disruption of the Epidermal Barrier Induces Regulatory T Cells via IL-33 in Mice

Delayed-type skin reaction to 2,4-dinitrophenylated epidermal cells in guinea pigs with contact sensitivity to 2,4-dinitrochlorobenzene

Contact Info

Product

Resources

About