The Surface Antigens of Orthopoxviruses Detected by Cross-neutralization Tests on Cross-absorbed Antisera

Baxby, Derrick

doi:10.1099/0022-1317-58-2-251

Cited by 17 publications

(5 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This single direction of cross-neutralization suggests that ERPV has several neutralizing antigenic epitopes, only some of which are shared with vaccinia virus and ectromelia virus. Similar results are reported in other Orthopoxvirus cross-neutralization studies (Baxby, 1982).…”

Section: Discussionsupporting

confidence: 92%

Further characterization of the biological and pathogenic properties of erythromelalgia-related poxviruses

Zheng

Specter

Jiang-Hong³

et al. 1992

Journal of General Virology

View full text Add to dashboard Cite

Six isolates of erythromelalgia-related poxvirus (ERPV) were characterized with respect to host range, c.p.e, and inclusions, pock formation on chorioallantoic membrane (CAM), morphogenesis, serological reactivity, pathogenesis in animals and DNA restriction fragment profile. The results suggest that ERPV is either a new member of the Orthopoxvirus genus or a subspecies of ectromelia virus. Evidence is provided that (i) ERPV has a wide host range in vitro in which characteristic viral c.p.e, and inclusion bodies are induced; (ii) ERPV, unlike ectromelia virus, causes the formation of tiny greyish-white pocks on CAM both at 34 °C and 39 *C; (iii) eosinophilic A-type inclusions of ERPV do not contain viral particles; (iv) ERPV isolates are neutralized by both rabbit anti-vaccinia virus and mouse anti-ectromelia virus sera, but not vice versa; (v) young rabbits are not susceptible to ERPV by skin and/or corneal scratch infection even though ERPV is lethal for mice by intraperitoneal inoculation; (vi) the HindlII and Sail fragment profiles ofERPV P-4 DNA are similar to, but obviously different from, those of Chinese ectromelia virus. These biological and pathogenic characteristics of ERPV are distinguishable from those of other members of the genus Orthopoxvirus currently described in the literature.

show abstract

Section: Discussionsupporting

confidence: 92%

Further characterization of the biological and pathogenic properties of erythromelalgia-related poxviruses

Zheng

Specter

Jiang-Hong³

et al. 1992

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…Abs in VIG recognize many antigen targets, including surface proteins of both EV and MV virion forms of VACV (Davies et al, 2005a). Study of polyclonal Abs in poxvirus-immune sera of rabbits revealed the pattern of recognition for each poxvirus was unique, but also suggested that different poxvirus species shared common neutralizing determinants (Baxby, 1982). Studies in murine infection models identified targets for neutralizing and protective mouse monoclonal Abs (mAbs), which included the MV surface proteins A27, L1, H3, D8, A28, A13 and A17, and the EV surface proteins B5 and A33 (Moss, 2011).…”

Section: Introductionmentioning

confidence: 99%

Cross-Neutralizing and Protective Human Antibody Specificities to Poxvirus Infections

Gilchuk

Sapparapu

et al. 2016

Cell

181

162

View full text Add to dashboard Cite

SUMMARY Monkeypox (MPXV) and cowpox (CPXV) are emerging agents that cause severe human infections on an intermittent basis, and variola virus (VARV) has potential for use as an agent of bioterror. Vaccinia immune globulin (VIG) has been used therapeutically to treat severe poxvirus infections, but is in short supply. We generated a large panel of orthopoxvirus-specific human monoclonal Abs from immune subjects to investigate the molecular basis of broadly neutralizing antibody responses for diverse orthopoxviruses. Detailed analysis revealed the principal neutralizing antibody specificities that are cross-reactive for VACV, CPXV, MPXV and VARV and that are determinants of protection in murine challenge models. Optimal protection following respiratory or systemic infection required a mixture of Abs that targeted several membrane proteins, including proteins on enveloped and mature virion forms of virus. This work reveals orthopoxvirus targets for human Abs that mediate cross-protective immunity and identifies new candidate Ab therapeutic mixtures to replace VIG.

show abstract

“…A relatively large number of serological diagnostic approaches have been used to identify orthopoxvirus infection, including hemagluttination inhibition ( Jezek et al 1987a), gel precipitation (Gispen 1955;, complement fixation , cross-neutralization (Baxby 1982), immunofluorescence (Gispen et al , 1976, Western blot (Demkowicz et al 1992;Jones-Trower et al 2005;Dubois and Slifka 2008), radioimmunoassay/ELISA (Hutchinson et al 1977;Marennikova et al 1981;Jezek et al 1987a;Karem et al 2005;Hammarlund et al 2005), or a modified post-adsorption ELISA (Dubois and Slifka 2008). Of these serological approaches, the ELISA technique is the least labor intensive and most likely to be amenable to high-throughput quantitative analysis.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Peptide-Based ELISA for Serological Diagnostics: A Retrospective Study of Human Monkeypox Infection

Dubois

Hammarlund

2012

Vector-Borne and Zoonotic Diseases

View full text Add to dashboard Cite

Although smallpox has been eradicated, other diseases caused by virulent orthopoxviruses such as monkeypox virus (MPV) remain endemic in remote areas of western and central sub-Saharan Africa, and represent a potential biothreat due to international travel and/or inadvertent exposure. Unfortunately, extensive antigenic cross-reactivity among orthopoxviruses presents a challenge to serological diagnosis. We previously reported a 20mer peptide-based ELISA that identified recent MPV infection with > 90% sensitivity and > 90% specificity. However, the sensitivity of this approach was not determined with samples obtained at later time points after antibody titers had declined from their peak levels. To improve assay sensitivity for detecting MPV-specific antibodies at later time points, we compared diagnostic 20mer peptides to 30mer peptides. In addition, optimal 30mer peptides were tested in combination or after conjugating selected peptides to a carrier protein (bovine serum albumin) to further improve assay performance. An optimized combination of four unconjugated 30mer peptides provided 100% sensitivity for detecting MPV infection at 2-6 months post-infection, 45% sensitivity for detecting MPV infection at > 2 years post-infection, and 99% specificity. However, an optimized combination of two peptide conjugates provided 100% sensitivity for detecting MPV infection at 2-6 months post-infection, 90% sensitivity for detecting MPV infection at > 2 years post-infection, and 97% specificity. Peptide-based ELISA tests provide a relatively simple approach for serological detection of MPV infection. Moreover, the systematic approach used here to optimize diagnostic peptide reagents is applicable to developing improved diagnostics to a broad range of other viruses, and may be particularly useful for distinguishing between closely-related viruses within the same genus or family.

show abstract

The Surface Antigens of Orthopoxviruses Detected by Cross-neutralization Tests on Cross-absorbed Antisera

Cited by 17 publications

References 18 publications

Further characterization of the biological and pathogenic properties of erythromelalgia-related poxviruses

Further characterization of the biological and pathogenic properties of erythromelalgia-related poxviruses

Cross-Neutralizing and Protective Human Antibody Specificities to Poxvirus Infections

Optimization of Peptide-Based ELISA for Serological Diagnostics: A Retrospective Study of Human Monkeypox Infection

Contact Info

Product

Resources

About