The surface charge of anti-bacterial coatings alters motility and biofilm architecture

Rzhepishevska, Olena; Hakobyan, Shoghik; Ruhal, Rohit; Gautrot, Julien E.; Barbero, David R.; Ramstedt, Madeleine

doi:10.1039/c3bm00197k

Cited by 171 publications

(190 citation statements)

References 48 publications

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“…This was mainly by reducing the depressions on the material surfaces by the crystallized deposition of rapamycin on the stent. The obtained results were found supportive when compared to the results of Olena Rzhepishevska et al 27 . The researchers found that the surface charge of antibacterial coatings was more efficient in reducing bacterial adhesion and biofilm formation.…”

Section: Discussionsupporting

confidence: 74%

Drug Eluting Stents Coated With Rapamycin Crystals for the Prevention of Restenosis and Biofilm Formation

Prasanth¹,

Saravanakumari²

2017

Int. J. Res. Ayurveda Pharm.

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Restenosis and biofilm formation during coronary stent implantation were considered to increase the complications of cardiovascular disease. To prevent these dual problems, drug-eluting stents coated with crystallized drug-polymer mixtures (Rapamycin-Polyvinyl pyrrolidone) were developed and its efficacy was investigated in the present study. Single layer coating using seeding and crystallization of drug-polymer mixture on the stent surface were carried out for effective drug delivery at the target site. Efficacy of rapamycin release concentration from the coated drug-eluting stents for the prevention of restenosis using High performance liquid chromatography was determined. Antibacterial activity of the drug-eluting stents against biofilm organisms was simultaneously studied. High performance liquid chromatography established the efficiency of modified coating technique and controlled release of rapamycin from drug-polymer mixture. Antibacterial activity of drug-eluting stents showed maximum inhibitory zone of 31.3mm against Staphylococcus aureus for 2X crystallized rapamycin coated stents. SEM images of rapamycin coatings on the stents exhibits extremely large uniform and continuous layer of parallelogram shaped rapamycin crystals in drug loaded stents. The sustained release of drugs fulfilled the primary aim of developing an anti-infective method for post-operative infection caused by biofilm organisms and also the prevention of restenosis.

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Section: Discussionsupporting

confidence: 74%

Drug Eluting Stents Coated With Rapamycin Crystals for the Prevention of Restenosis and Biofilm Formation

Prasanth¹,

Saravanakumari²

2017

Int. J. Res. Ayurveda Pharm.

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“…The ability of bacteria to adhere to natural and artificial materials is a first step in biofilm formation, is central for bacterial survival, and has great clinical and environmental relevance [1][2][3]. Initial adhesion is dependent on a large number of parameters [4,5].…”

Section: Introductionmentioning

confidence: 99%

A multivariate approach to correlate bacterial surface properties to biofilm formation by lipopolysaccharide mutants of Pseudomonas aeruginosa

Ruhal

Antti

Rzhepishevska

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Bacterial biofilms are involved in various medical infections and for this reason it is of great importance to better understand the process of biofilm formation in order to eradicate or mitigate it. It is a very complex process and a large range of variables have been suggested to influence biofilm formation. However, their internal importance is still not well understood. In the present study, a range of surface properties of Pseudomonas aeruginosa lipopolysaccharide mutants were studied in relation to biofilm formation measured in different kinds of multi-well plates and growth conditions in order to better understand the complexity of biofilm formation. Multivariate analysis was used to simultaneously evaluate the role of a range of physiochemical parameters under different conditions. Our results suggest the presence of serum inhibited biofilm formation due to changes in twitching motility. From the multivariate analysis it was observed that the most important parameters, positively correlated to biofilm formation on two types of plates, were high hydrophobicity, near neutral zeta potential and motility. Negative correlation was observed with cell aggregation, as well as formation of outer membrane vesicles and exopolysaccharides. This work shows that the complexity of biofilm formation can be better understood using a multivariate approach that can interpret and rank the importance of different factors being present simultaneously under several different environmental conditions, enabling a better understanding of this complex process.

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“…The FC270 system is particularly useful for the analysis of resulting biofilms using confocal laser scanning microscopy (CLSM). In addition, the FC270 system has the advantage to study biofilm formation in different materials and surfaces [51]. However, there are three major limitations, as they are, a limited visualization the biofilm formation during development, only two biofilms can be J Microbial Biochem Technol ISSN:1948-5948 JMBT, an open access journal Bacteria: Biochemical Physiology inability to collect effluent for analysis of metabolic products generated during biofilm growth [57][58][59].…”

Section: Fc270 Flow-cell Systemmentioning

confidence: 99%

“…Using these devices is possible to detect different analytes between microbes within biofilm. The major advantages of biosensing system are its specificity, sensitivity, experimental reproducibility, as well as ability to analyte detection in optically opaque solutions, also the application both for the study of static and flow biofilms [51][52][53].…”

Section: Biofilm Biosensormentioning

confidence: 99%

Anti-Biofilm Drug Susceptibility Testing Methods: Looking for New Strategies against Resistance Mechanism

Bueno¹

2011

JMBT

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Biofilm is a reservoir of drug resistant microorganisms that can increase the failure rate of anti-infective therapy and is a public health concern. Antibiofilm drug discovery is necessary for developing new drugs, biocides and wound management protocols. This makes the standardization and implementation of in vitro antibiofilm screening platforms a challenge in the search for new antibiotics, because current antimicrobials are active against planktonic bacteria and have poor diffusion across biofilm matrix. Usually, based in the research topic, the antibiofilm methods have been classified in static and flow depending of continuous supply of nutrients that affect the microbial growth, the final aim of these assays is obtain Minimal Biofilm Inhibitory Concentration (MBIC) and Minimal Biofilm Eradication Concentration (MBEC) values as efficacy parameter of the compound or procedure evaluated, but is very important correlates data from different models in order to give real results of activity. This review aims at describing the initial tools for to establishing an antibiofilm drug discovery-prospecting program.

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The surface charge of anti-bacterial coatings alters motility and biofilm architecture

Cited by 171 publications

References 48 publications

Drug Eluting Stents Coated With Rapamycin Crystals for the Prevention of Restenosis and Biofilm Formation

Drug Eluting Stents Coated With Rapamycin Crystals for the Prevention of Restenosis and Biofilm Formation

A multivariate approach to correlate bacterial surface properties to biofilm formation by lipopolysaccharide mutants of Pseudomonas aeruginosa

Anti-Biofilm Drug Susceptibility Testing Methods: Looking for New Strategies against Resistance Mechanism

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