The survival and hepatic and muscle glucose and lactate levels of Rhamdia quelen inoculated with Aeromonas hydrophila and treated with terpinen-4-ol, carvacrol or thymol

Cunha, Jessyka Arruda da; Bandeira, Guerino; Silva, Elisia Gomes da; Scheeren, Cecília de Ávila; Fausto, Viviane Pedroso; Salbego, Joseânia; Vaucher, Rodrigo de Almeida; Vargas, Agueda Castagna de; Baldisserotto, Bernardo

doi:10.1016/j.micpath.2018.12.005

Cited by 16 publications

(7 citation statements)

References 28 publications

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“…Although the MIC reported by G. Bandeira et al was lower than that reported in this study [36], the hemolytic and biofilm formation inhibitory concentration was higher than in this study. As shown in Figure 2B, the hemolytic inhibitory concentration was about 2.5 times lower than that reported by G. Bandeira et al [35]. Different cultivation time and strains might result in different hemolytic and biofilm inhibitory activity.…”

Section: Discussionmentioning

confidence: 54%

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Thymol Protects Channel Catfish from Aeromonas hydrophila Infection by Inhibiting Aerolysin Expression and Biofilm Formation

et al. 2020

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Aeromonas hydrophila is an opportunistic pathogen responsible for a number of diseases in freshwater farming. Moreover, the bacterium has been identified as a zoonotic pathogen that threatens human health. Antibiotics are widely used for treatments of infectious diseases in aquaculture. However, the abuse of antibiotics has led to the emergence of antimicrobial resistant strains. Thus, novel strategies are required against resistant A. hydrophila strains. The quorum sensing (QS) system, involved in virulence factor production and biofilm formation, is a promising target in identifying novel drugs against A. hydrophila infections. In this study, we found that thymol, at sub-inhibitory concentrations, could significantly reduce the production of aerolysin and biofilm formation by inhibiting the transcription of genes aerA, ahyI, and ahyR. These results indicate that thymol inhibits the quorum sensing system. The protective effects of thymol against A. hydrophila mediated cell injury were determined by live/dead assay and lactate dehydrogenase (LDH) release assay. Moreover, the in vivo study showed that thymol could significantly decrease the mortality of channel catfish infected with A. hydrophila. Taken together, these findings demonstrate that thymol could be chosen as a phytotherapeutic candidate for inhibiting quorum sensing system-mediated aerolysin production and biofilm formation in A. hydrophila.

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Section: Discussionmentioning

confidence: 54%

“…The findings indicated that thymol is safe for fish in doses lower than 100 mg/kg. Studies published by Morselli et al [34] and Cunha et al [35] demonstrated that thymol could decrease A. hydrophila-mediated mortality in grass carp and Rhamdia quelen. However, the influence of thymol on A. hydrophila has not been well studied.…”

Section: Discussionmentioning

confidence: 98%

Thymol Protects Channel Catfish from Aeromonas hydrophila Infection by Inhibiting Aerolysin Expression and Biofilm Formation

et al. 2020

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“…sidoides , but in different quantities: carvacrol (49.7%), p ‐cymene (13.3%) and thymol (9.9%) (Oliveira et al, 2018). Da Cunha et al (2019) reported that R . quelen subjected to baths with the compound thymol (i.e.…”

Section: Discussionmentioning

confidence: 99%

Short‐term baths with essential oils of Lippia sidoides , Ocimum gratissimum and Zingiber officinale influence blood parameters and survival of tambaqui ( Colossoma macropomum ) after infection with Aeromonas hydrophila

Monteiro

Oliveira

Brandão

et al. 2021

Aquaculture Research

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The fish farm has presented rapid advance in the last years, and fish consumption provides one of the main source of proteins and nutrients that are essential for human health (FAO, 2020). The Brazilian native fish that include Colossoma macropomum, popularly known as tambaqui, Piaractus mesopotamicus (pacu) and their hybrids, contributed with 278,671 tonnes or 34.7% of the total fish production (PEIXE BR, 2021). Among the main native farmed species, C. macropomum stands out due to the availability technologies for production

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“…Other fishes have shown mixed responses following inoculation with aeromonads. Inoculation with A. hydrophila caused an increased glucose response in Rohu Labeo rohita (Pal et al, 2015) whereas there was not an increase in liver glucose in Silver Catfish Rhamdia quelen (da Cunha et al, 2019). Unlike glucose, plasma lactate levels were higher in control fish, possibly indicating reduced activity and increased immune system energy expenditure in A. sobria treated fish (Wakabayashi & Iwado, 1985).…”

Section: Discussionmentioning

confidence: 99%

Effects of Aeromonas sobria on physiology and fillet quality of market‐sized Channel Catfish Ictalurus punctatus

Keretz

Peterman

Petrie‐Hanson

et al. 2021

J World Aquaculture Soc

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Channel Catfish Ictalurus punctatus fillets are rejected by processing plants because of diffuse red discoloration, but the cause of the red discoloration is currently unknown. Previous studies have shown a correlation between Aeromonas sobria and the occurrence of red fillet, although causation has not been established and little is known of the effects of A. sobria on catfish. Therefore, the present study inoculated Channel Catfish with A. sobria to determine whether inoculation induced red discoloration in fillets. Visual examination of the muscle of fish inoculated with A. sobria showed diffuse red blotches around the inoculation area and muscle histology showed increased presence of leukocytes and red blood cells, edema, and necrosis. Inoculation with A. sobria also resulted in necrosis of hematopoietic tissue, interstitial hemorrhage and melanomacrophage aggregates in the posterior kidney, and inflammation in the anterior kidney and spleen. These findings demonstrate intramuscular inoculation of A. sobria can result in red fillets and impact hematopoietic and erythrocytic tissues. Further research will be necessary to assess red fillet prevention and control for both catfish farmers and processors.

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The survival and hepatic and muscle glucose and lactate levels of Rhamdia quelen inoculated with Aeromonas hydrophila and treated with terpinen-4-ol, carvacrol or thymol

Cited by 16 publications

References 28 publications

Thymol Protects Channel Catfish from Aeromonas hydrophila Infection by Inhibiting Aerolysin Expression and Biofilm Formation

Thymol Protects Channel Catfish from Aeromonas hydrophila Infection by Inhibiting Aerolysin Expression and Biofilm Formation

Short‐term baths with essential oils of Lippia sidoides , Ocimum gratissimum and Zingiber officinale influence blood parameters and survival of tambaqui ( Colossoma macropomum ) after infection with Aeromonas hydrophila

Effects of Aeromonas sobria on physiology and fillet quality of market‐sized Channel Catfish Ictalurus punctatus

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