The Susceptibility of &lt;i&gt;Clostridium perfringens&lt;/i&gt;Type A to Cephalosporin C Antibiotics &lt;i&gt;in vitro&lt;/i&gt;

Traub, Walter H.

doi:10.1159/000220710

Cited by 12 publications

(8 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Any of the penicillins we tested should constitute effective therapy against C. perfringens. Our findings with cephalosporins agree with published results for cefamandole, cephalexin, cephaloridine, and cephalothin (16,17). Cefazolin, cefoxitin, and cephradine showed activity comparable to the other cephalosporins.…”

Section: Resultssupporting

confidence: 93%

Susceptibility of Clostridium perfringens Isolated from Human Infections to Twenty Antibiotics

Schwartzman

Reller

Wang

1977

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The proper choice of antibiotic for Clostridium perfringens infections in patients allergic to penicillin is not dear; the usual recommendations and recent in vitro studies disagree. We tested the susceptibility of 57 strains ofC. perfringens to eight penicillins, seven cephalosporins, two tetracyclines, clindamycin, chloramphenicol, and rifampin by the agar dilution method. All strains were inhibited by (per milliliter) 4 ,ug or less of any of the penicillins, chloramphenicol, or clindamycin and 8 ,mg or less of any of the cephalosporins tested. Penicillin G and amoxicillin inhibited all strains at 0.12 ,g or less per ml. Only 54% of the strains were inhibited by 1 ,ug of tetracycline per ml. Penicillin G remains the drug of first choice for infections with C. perfringens; it need not be added to a regimen containing a penicillinase-resistant penicillin given parenterally in high doses. The cephalosporins should be considered as alternative drugs for penicillin-allergic patients. Clindamycin and chloramphenicol are also effective. Tetracyclines cannot be depended upon in clostridial infections without in vitro testing, which is impracticable for initial empirical therapy.Penicillin G is the antibiotic of first choice for treating cellulitis, sepsis, and myonecrosis caused by Clostridium perfringens (1, 2,8,11,18). Although erythromycin and tetracycline are the alternative drugs usually recommended (1, 2,8,11,18), 10 to 30% of C. perfringens are resistant to tetracycline, and a few strains are also resistant to erythromycin (6,8,9,13). Cephalosporins and penicillinase-resistant and other semisynthetic penicillins are used for contaminated wounds after severe trauma and for suspected sepsis in patients with cancer, who are also at a higher risk for clostridial infections (3,4,14). Because of both scanty information about many of these agents and the need for a safe antibiotic for penicillin-allergic patients, we studied the activity of 20 antibiotics against strains of C. perfringens isolated from human infections or feces. Susceptibility testing. Agar dilution tests were done by the method of Sutter and Washington (15). MATERIALSAntibiotics were diluted in twofold steps above and below 1 pg/ml in Brucella agar (Pfizer) supplemented with 5% laked sheep blood and vitamin K1 (10 ug/ml). The inoculum was adjusted to a no. 1 MacFarland turbidity standard (3 x 108 colonyforming units) and then diluted with prereduced Carey-Blair buffer, so that each spot delivered by 695 on May 9, 2018 by guest http://aac.asm.org/ Downloaded from

show abstract

Section: Resultssupporting

confidence: 93%

Susceptibility of Clostridium perfringens Isolated from Human Infections to Twenty Antibiotics

Schwartzman

Reller

Wang

1977

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…Pre-vious studies have shown that this organism is susceptible in vitro to a variety of commonly used antimicrobial agents (2,5,6,8,10,15,16). However, the constant, widespread use of antibiotics in the practice of both human and veterinary medicine necessarily entails the risk of the development of antibiotic resistance among some strains of C. perfringens.…”

mentioning

confidence: 99%

Standardized Antimicrobial Disc Susceptibility Testing of Anaerobic Bacteria: In Vitro Susceptibility of Clostridium perfringens to Nine Antibiotics

Sapico

Kwok

Sutter

et al. 1972

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The in vitro susceptibility of 43 strains of Clostridium perfringens to nine antibiotics was determined by a standardized test for rapid-growing anaerobes. Good correlation was established between the agar dilution susceptibility and the disc diffusion susceptibility results. The inhibition zone diameters around the antibiotic discs, however, were generally much smaller than those of gram-negative anaerobes previously studied. All of the strains tested were susceptible in vitro to chloramphenicol, clindamycin, doxycycline, minocycline, penicillin, and vancomycin. Erythromycin showed poor in vitro activity against this organism, with only 7% of the strains susceptible, 72% intermediate in susceptibility, and 21%o resistant. In tests of the 43 strains against lincomycin, 58% were susceptible, 32.5°% were intermediate in susceptibility, and 9.5% were resistant. Against tetracycline, 37% of the strains were intermediate in susceptibility and the rest were susceptible.

show abstract

“…The Bauer-Kirby disk diffusion method was performed as before [1,6] em ploying Mueller-Hinton agar (Difco) with and without 5 %> sheep blood. The agar dilution as well as the microtiter broth dilution tests for clostridial isolates were performed according to the previously described techniques [6,8], Tube dilution tests for the 3 laboratory control strains were done as reported elsewhere [7], The MIC (minimal inhibitory concentration) was defined as the lowest concentration of gentamicin completely inhibiting growth as judged by visual inspection; the MBC (minimal bactericidal concentration) was the lowest concentration of anti biotic yielding no colonies from 3-mm loopful samples (roughly 0.03 ml) streaked to quarter sectors of sheep blood agar plates that were incubated anaerobically.…”

Section: Sensitivity Test Methodsmentioning

confidence: 99%

“…The same 30 clinical isolates of C. perfringens and the control strain of Sta phylococcus aureus that had been employed previously [6] were used in this study; additional control strains used were S. aureus 209 P and a strain of Escherichia coli, both of known antibiotic susceptibility.…”

Section: Strains Of C Perfringens Type Amentioning

confidence: 99%

In vitro Resistance of Clostridium perfringens Type A to Gentamicin Sulfate, and Reduced Activity of the Antibiotic under Anaerobic Atmospheric Conditions

Traub¹,

Raymond²

1971

Chemotherapy

Self Cite

View full text Add to dashboard Cite

Thirty clinical isolates of C. perfringens type A were found to be resistant to gentamicin sulfate in vitro, as determined by the disk diffusion and agar and broth dilution methods of sensitivity testing. Two isolates (6.7%) required 12 μg/ml for inhibition, while the remaining 28 isolates (93.3%) were inhibited by 25–100 μg/ml of gentamicin. The activity of gentamicin sulfate was found to be depressed roughly twofold under anaerobic conditions of incubation. The significance of these findings is discussed.

show abstract

The Susceptibility of <i>Clostridium perfringens</i>Type A to Cephalosporin C Antibiotics <i>in vitro</i>

Cited by 12 publications

References 5 publications

Susceptibility of Clostridium perfringens Isolated from Human Infections to Twenty Antibiotics

Susceptibility of Clostridium perfringens Isolated from Human Infections to Twenty Antibiotics

Standardized Antimicrobial Disc Susceptibility Testing of Anaerobic Bacteria: In Vitro Susceptibility of Clostridium perfringens to Nine Antibiotics

<i>In vitro</i> Resistance of <i>Clostridium perfringen</i><i>s</i> Type A to Gentamicin Sulfate, and Reduced Activity of the Antibiotic under Anaerobic Atmospheric Conditions

Contact Info

Product

Resources

About