The sustainable mitigation of in vitro ruminal biogas emissions by ensiling date palm leaves and rice straw with lactic acid bacteria and Pleurotus ostreatus for cleaner livestock production

Abstract

Aims:The sustainable utilization of date palm leaves (DPL) and rice straw (RS) as feed materials for ruminant was evaluated using an in vitro wireless gas production (GP) technique. Methods and Results: DPL and RS were individually ensiled with lactic acid bacteria (LAB) for 45 days or used as substrates for the cultivation of Pleurotus ostreatus (PO) mushroom for 35 days. A total mixed ration was formulated as a control ration.In the other rations, berseem hay replaced DPL (ensiled without additives or ensiled with LAB or PO) at 25%, 50%, 75% and 100%. Ensiling with LAB did not affect the chemical composition of DPL or RS, while PO treatment reduced their fibre fraction contents. Ensiling without additives lowered (p < 0.05) the asymptotic production of total gas, methane (CH 4 ) and carbon dioxide (CO 2 ), and the rate of CH 4 and CO 2 while increasing (p < 0.05) the lag time of CH 4 and CO 2 production. Ensiling of materials with LAB and treatment with PO decreased (p < 0.05) the asymptotic production of total gas, CH 4 and CO 2 production and decreased the rate of CH 4 and CO 2 production. Ensiling without additives decreased (p < 0.05) total bacterial count, and increased (p < 0.05) fermentation pH and total volatile fatty acids (VFA), while LAB-ensiled DPL increased (p < 0.05) total VFA and propionate concentrations and decreased total protozoal count. The PO-treated DPL decreased (p < 0.05) bacterial count, protozoal count and fermentation pH and increased total VFA production. Conclusions:Replacing berseem hay with LAB or PO-treated DPL at 25% increased GP; however, increased CH 4 and CO 2 production, whereas the other replacement levels decreased total gas, CH 4 and CO 2 production. The treatment with LAB is more recommended than the PO treatment.

The effects of replacement of berseem hay in total mixed rations with date palm leaves ensiled with malic or lactic acids at different levels on the nutritive value, ruminal in vitro biogas production and fermentation

“…Date palm (Phoenix dactilifera) leaves (DPL) are an example of the agricultural byproducts with a considerable potential nutritive value, but unsatisfactory use [2]. The nutritive value of DPL is hindered due to high concentration of fiber [3]. With about 20 kg of leaves produced from each tree, 14 million date palm trees generate approximately 650,000 tons of dry matter (DM) annually in Egypt [4].…”

A Combination of Novel Microecological Agents and Molasses Role in Digestibility and Fermentation of Rice Straw by Facilitating the Ruminal Microbial Colonization

“…A recent study has also confirmed that LAB inoculants in corn silage can improve the total VFA concentration in the rumen (Monteiro et al, 2021 ), compared with the control group, the increase was 13.7%. Kholif et al ( 2021 ) also reported that the addition of LAB to date palm leaves could increase the total VFA in the rumen. Similar studies were reported in wheat straw silage that the additive LAB can improve the acetic acid and total VFA concentration of wheat straw silage (Babaeinasab et al, 2015 ).…”

“…Zhang Q. et al ( 2016 ) documented that the addition of LAB reduced the ADF and NDF contents in L. chinensis silage. The pH value is one of the key indexes to measure the quality of fermentation during silage fermentation (Kholif et al, 2021 ). The low pH value during silage fermentation means that the activity of harmful microorganisms was inhibited and reduced the loss of nutrients (Fan et al, 2021 ).…”

Utilization of Waste Date Palm Leaves Biomass Ensiled with Malic or Lactic Acids in Diets of Farafra Ewes under Tropical Conditions

“…In Egypt, approximately 650,000 tons of leaves’ dry matter (DM) is produced annually from about 14 million trees [ 3 , 4 ]. DPL are not properly utilized due to high fiber (43–73% neutral detergent fiber (NDF) [ 5 ]) and low crude protein (CP) (4.2 to 16.5% of DM [ 6 , 7 ]) contents and low nutritive value and digestibility. Ensiling low-quality forages with silage additives is common to improve their fermentation profile and nutritive value and to reduce adverse bacterial growth and negative effects of some antinutritional factors in feeds [ 8 ].…”