The SV40 small t intron is accurately and efficiently spliced in tobacco cells

Hunt, Arthur G.; Mogen, Bradley D.; Chu, Nathan M.; Chua, Nam‐Hai

doi:10.1007/bf00023989

Cited by 21 publications

(11 citation statements)

References 12 publications

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“…The only heter-ologous intron that is known to be accurately spliced in plants (tobacco) is the 66 base A Urich intron of the primate virus SV40 t-antigen mRNA (Hunt et al, 1991). Our dicot models give a high donor score of 0.995 (with X U À 0.16 and X CG 0.20) paired with a high acceptor score of 0.85 (X U 0.26, X CG À 0.22), predicting that this viral intron would be correctly recognized in tobacco.…”

Section: Splicing Of Genes Of Animal Origin In Plant Cellsmentioning

confidence: 99%

Prediction of splice sites in plant pre-mRNA from sequence properties

Brendel

Kleffe

Carle-Urioste

et al. 1998

Journal of Molecular Biology

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Heterologous introns are often inaccurately or inef®ciently processed in higher plants. The precise features that distinguish the process of premRNA splicing in plants from splicing in yeast and mammals are unclear. One contributing factor is the prominent base compositional contrast between U-rich plant introns and¯anking G C-rich exons. Inclusion of this contrast factor in recently developed statistical methods for splice site prediction from sequence inspection signi®cantly improved prediction accuracy. We applied the prediction tools to re-analyze experimental data on splice site selection and splicing ef®ciency for native and more than 170 mutated plant introns. In almost all cases, the experimentally determined preferred sites correspond to the highest scoring sites predicted by the model. In native genes, about 90% of splice sites are the locally highest scoring sites within the bounds of the¯anking exon and intron. We propose that, in most cases, local context (about 50 bases upstream and downstream from a potential intron end) is suf®cient to account for intrinsic splice site strength, and that competition for transacting factors determines splice site selection in vivo. We suggest that computer-aided splice site prediction can be a powerful tool for experimental design and interpretation.

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Section: Splicing Of Genes Of Animal Origin In Plant Cellsmentioning

confidence: 99%

Prediction of splice sites in plant pre-mRNA from sequence properties

Brendel

Kleffe

Carle-Urioste

et al. 1998

Journal of Molecular Biology

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“…A small number of introns were <70 nt and therefore smaller than the minimum of 70-73 nt length established for efficient splicing in plants [5]. However, the AU-rich SV40 small t intron was efficiently spliced in tobacco and is only 66 nt in length [8]. Taking this length as a minimum, only three introns are reported as being smaller: the tentatively identified intron 5 of ubiquitin conjugating enzyme, UBC5 (65 nt) [26], intron 10 of an anthranilate synthase alpha subunit gene (63 nt) which is poorly expressed [ 19] and intron 2 of cytochrome C (59 nt).…”

mentioning

confidence: 91%

Arabidopsis consensus intron sequences

Brown¹,

Smith²,

Simpson³

1996

Plant Mol Biol

122

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We have analysed 998 Arabidopsis intron sequences in the EMBL database. All Arabidopsis introns to adhere to the :GU ... AG: rule with the exception of 1% of introns with :GC at their 5' ends. Virtually all of the introns contained a putative branchpoint sequence (YUNAN) 18 to 60 nt upstream of the 3' splice site. Although a polypyrimidine tract was much less apparent than in vertebrate introns, the most common nucleotide in the region upstream of the 3' splice site was uridine. Consensus sequences for 5' and 3' splice sites and branchpoint sequences for Arabidopsis introns are presented.

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“…Nevertheless, animal introns are not processed in any plant tissue so far examined (26,27). The only exception is the simian virus 40 small t-antigen intron, which is very small and AU-rich (28). In contrast, plant introns are normally recognized in animal cells or extracts, suggesting the requirement of more specific sequences for intron recognition in plants (27,29,30).…”

mentioning

confidence: 99%

Pre-mRNA splicing in plants: characterization of Ser/Arg splicing factors.

Lopato

Mayeda

Krainer

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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The fact that animal introns are not spliced out in plants suggests that (1,2).Among the few well-characterized metazoan splicing factors are the proteins SF2/ASF, which is involved in selection of the 5' splice site, and U2AF, which stimulates the interaction of U2 snRNP with the branch point region. It has been shown that these and other splicing factors contain a similar structural motif, termed the RS domain (3). These proteins are phosphorylated in vivo and share a serine phosphoepitope recognized by monoclonal antibody mAbl04 (4, 5). They were termed SR proteins and comprise a family of evolutionary conserved nuclear phosphoproteins, which contain either one or two RNA recognition motifs and sequences of alternating serine and arginine (SR) residues (3,5

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The SV40 small t intron is accurately and efficiently spliced in tobacco cells

Cited by 21 publications

References 12 publications

Prediction of splice sites in plant pre-mRNA from sequence properties

Prediction of splice sites in plant pre-mRNA from sequence properties

Arabidopsis consensus intron sequences

Pre-mRNA splicing in plants: characterization of Ser/Arg splicing factors.

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