The SWGEDWGEIW from Soybean Peptides Reduce Oxidative Damage-Mediated Apoptosis in PC-12 Cells by Activating SIRT3/FOXO3a Signaling Pathway

Yi, Guofu; Zhou, Mengyue; Du, Qingfei; Yang, Shuibing; Zhu, Yuxia; Dong, Yining; Liu, Yang; Li, He; Li, You; Liu, Xinqi

doi:10.3390/molecules27217610

Cited by 2 publications

(1 citation statement)

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“…SBPs regulate tryptophan hydroxylase (THP) and serotonin-N-acetyltransferase (AANAT) proteins to increase sleep [ 39 ]. SWGEDWGEIW (the single peptide, TSP) in SBPs reduces oxidative damage-mediated apoptosis in PC-12 cells by activating the SIRT3/FOXO3a signaling pathway [ 40 ]. TSP has a good inhibitory effect on hydrogen peroxide-induced inflammation, but it is unclear how TSP affects insulin resistance in adipocytes in this state of inflammation.…”

Section: Introductionmentioning

confidence: 99%

The SWGEDWGEIW from Soybean Peptides Reduces Insulin Resistance in 3T3-L1 Adipocytes by Activating p-Akt/GLUT4 Signaling Pathway

Sang

Zhu

et al. 2023

Molecules

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Diabetes mellitus, a group of metabolic disorders characterized by persistent hyperglycemia, affects millions of people worldwide and is on the rise. Dietary proteins, from a wide range of food sources, are rich in bioactive peptides with anti-diabetic properties. Notably, the protective mechanism of the single peptide SWGEDWGEIW (TSP) from soybean peptides (SBPs) on insulin resistance of adipocytes in an inflammatory state was investigated by detecting the lipolysis and glucose absorption and utilization of adipocytes. The results showed that different concentrations of TSP (5, 10, 20 µg/mL) intervention can reduce 3T3-L1 adipocytes’ insulin resistance induced by inflammatory factors in a dose-dependent manner and increase glucose utilization by 34.2 ± 4.6%, 74.5 ± 5.2%, and 86.7 ± 6.1%, respectively. Thus, TSP can significantly alleviate the lipolysis of adipocytes caused by inflammatory factors. Further mechanism analysis found that inflammatory factors significantly reduced the phosphorylation (p-Akt) of Akt, two critical proteins of glucose metabolism in adipocytes, and the expression of GLUT4 protein downstream, resulting in impaired glucose utilization, while TSP intervention significantly increased the expression of these two proteins. After pretreatment of adipocytes with PI3K inhibitor (LY294002), TSP failed to reduce the inhibition of p-Akt and GLUT4 expression in adipocytes. Meanwhile, the corresponding significant decrease in glucose absorption and the increase in the fat decomposition of adipocytes indicated that TSP reduced 3T3-L1 adipocytes’ insulin resistance by specifically activating the p-Akt/GLUT4 signal pathway. Therefore, TSP has the potential to prevent obesity-induced adipose inflammation and insulin resistance.

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Section: Introductionmentioning

confidence: 99%