The Sympathetic Postganglionic Fibre and the Block by Bretylium; The Block Prevented by Hexamethonium and Imitated by Mecamylamine

Burn, J. H.; Gibbons, W. R.

doi:10.1111/j.1476-5381.1964.tb01709.x

Cited by 24 publications

(19 citation statements)

References 19 publications

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“…After cold storage, tyramine-induced relaxation could not be demonstrated. Other investigations provided evidence to support the adrenergic nature of the relaxation produced by nicotine and DMPP (Gillespie & Mackenna, 1960;Greef, Kasperat & Oswald, 1962;Jarrett, 1962;Burn & Gibbons, 1964;Weiss, 1962). These results strongly support the view that loss of inhibitory response to nicotine, DMPP, and tyramine is probably due to deterioration of the adrenergic nerve structures during cold storage.…”

Section: Discussionsupporting

confidence: 79%

The effect of cold storage on the adrenergic mechanisms of intestinal smooth muscle

Hattori

Kurahashi

Mori

et al. 1972

British J Pharmacology

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Summary1. In the guinea-pig taenia caecum, fluorescent adrenergic fibres terminate in both muscle layers. The density of these fibres is greater in the taenia than in the underlying circular muscle layer. The myenteric plexus and individual ganglion cells are also densely innervated by intensely fluorescent adrenergic nerve fibres. 2. After three days of cold storage, the specific fluorescence disappeared from all tissue layers of the taenia caecum and smooth muscle fibres. In contrast, cholinesterase active substances were still demonstrable in all tissue layers even after seven days of cold storage but the density of these substances was decreased. 3. Cold storage (3-7 days) decreased the tissue noradrenaline content and did not modify the cholinesterase enzyme activity (4 days). 4. In cold stored strips, the inhibitory response to nicotine, 1,1-dimethyl-4-phenylpiperazinium iodide (DMPP) or electrical transmural stimulation was abolished and enhancement of the contractile response occurred. Cold storage also inhibited the inhibitory action of tyramine. Similar results were observed after reserpine treatment. 5. In fresh taenia, the relaxation produced by nicotine, DMPP and electrical transmural stimulation was inhibited by adrenoceptor blocking agents and bretylium. In cold storage preparations, contraction produced by these stimuli was blocked by parasympathetic blocking agents and potentiated by anticholinesterase. These results indicate that the inhibitory response to these stimulants is mediated by stimulation of the adrenergic nerve system more than by non-adrenergic nerves; the excitatory effect is probably due to stimulation of cholinergic nerves. 6. These results suggest that the adrenergic mechanisms of the taenia caecum are more labile in cold storage than the cholinergic mechanisms. Thus, the inhibitory action of cold storage on the relaxation produced by nicotine, DMPP, and transmural stimulation is probably explained by selective physical degeneration of the adrenergic nerve terminal. Also, enhancement of the contractile response to these stimulants in cold stored preparations is explained by the lack of adrenergic inhibitory mechanisms.

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Section: Discussionsupporting

confidence: 79%

The effect of cold storage on the adrenergic mechanisms of intestinal smooth muscle

Hattori

Kurahashi

Mori

et al. 1972

British J Pharmacology

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“…In the rectal caecum relaxations produced by nicotine were much more marked. Relaxations produced by ganglion stimulants in intestinal preparations are well known (Kuroda, 1917;Ambache & Edwards, 1951;Ambache, 1951;Evans & Schild, 1953;Jarrett, 1962;Greeff, Kasperat & Osswald, 1962;Weiss, 1962;Burn & Gibbons, 1964;Bucknell & Whitney, 1964;Fishlock & Parks, 1966;Burnstock, Campbell & Rand, 1966). Such relaxations produced by nicotine in preparations from other species are usually revealed or potentiated in the presence of atropine, but in the chick gut the contractile action of nicotine, TMA and DMPP were often resistant to the blocking action of atropine and hyoscine, and little or no potentiation of the relaxations occurred.…”

Section: Discussionmentioning

confidence: 99%

Pharmacological Responses of the Isolated Innervated Intestine and Rectal Caecum of the Chick

Everett

1968

British Journal of Pharmacology and Chemotherapy

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“…Weis (1962) made the same observation with reserpine in the guinea-pig taenia coli. Moreover, other workers have found that these inhibitory responses of intestinal preparation to nicotinic agents were abolished by bretylium (Greeff et al 1962, Jarrett 1962, Burn and Gibbons 1964. Burnstock et al (1966) observed in guinea-pig taenia coli that the relaxation produced by DMPP was abolished after high concentrations of adrenergic neuron blocking agents were applied.…”

Section: Discussionmentioning

confidence: 99%

Contractile Properties of Organ-cultured Intestinal Smooth Muscle

Rikimaru

1971

Tohoku J. Exp. Med.

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RIKIMARUA. Contractile properties of organ cultured intestinal snwoth muscle. Tohoku J. exp. Med., 1971, 103 (4), 317-329 -The contractile properties of the smooth muscle of guinea-pig taenia coli in organ culture were investigated in relation to the histochemical observation on its innervation.The smooth muscle preparations were cultured in the medium rich in horse serum and chick embryo extract. They preserved responsiveness to nicotine and transmural stimulation at low frequencies for more than three weeks. On the other hand, those cultured in the medium contained only lower concentration of horse serum and deficient in chick embryo extract lost such responsiveness after two to three weeks of incuba tion. It is evident from these data that the latter condition of culture would be adequate for the experiment in which the smooth muscle is maintained but the nerve element have to be eliminated.In this condition, the preparations on the 4th day of culture failed to produce a relaxation by transmural stimulation at high frequencies, though it was produced at low frequencies and by nicotine. The fluorescence of catecholamines disappeared at the same stage of culture, but no supersensitivity to noradrenaline and acetylcholine was observed. Histofluoro metric observation of the taenia before culture revealed the presence of intensely fluorescent nerve fibers scattered in the muscular layers and around the nonfluorescent ganglion cells in Auerbach's plexus. Neither fluorescent ganglion cells nor characteristic fluorescence of 5-HT could be observed in Auerbach's plexus. These results strongly confirmed the presence of non-adrenergic inhibitory neurons within the taenia.Histofluorometric data ruled out the possibilities of participa tion of dopamine and 5-HT in this inhibitory mechanism.The transmitter of the intramural inhibitory neurons did not show any fluorescence by the treatment with formaldehyde vapor. organ culture; taenia coli; contractile properties; catecholamine fluorescence Since Harrison (1907) first introduced the method of tissue culture to prove the "neuron theory" , tissue culture and organ culture have been widely used and developed by many investigators in various fields.The changes in form and behavior of muscle in tissue culture have since at tracted attention of investigators, and a good deal of works on tissue cultures of skeletal and cardiac muscles have been carried out. But the reports on smooth muscle in tissue culture and organ culture, especially in organ culture, are rela tively scarce (see Murray 1965). It must be admitted, therefore, that tissue cul-

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The Sympathetic Postganglionic Fibre and the Block by Bretylium; The Block Prevented by Hexamethonium and Imitated by Mecamylamine

Cited by 24 publications

References 19 publications

The effect of cold storage on the adrenergic mechanisms of intestinal smooth muscle

The effect of cold storage on the adrenergic mechanisms of intestinal smooth muscle

Pharmacological Responses of the Isolated Innervated Intestine and Rectal Caecum of the Chick

Contractile Properties of Organ-cultured Intestinal Smooth Muscle

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