The synergistic effect of Canady Helios cold atmospheric plasma and a FOLFIRINOX regimen for the treatment of cholangiocarcinoma in vitro

Jones, Olivia; Cheng, Xiaoqian; Murthy, Saravana R. K.; Ly, Lawan; Zhuang, Taisen; Basadonna, Giacomo; Keidar, Michael; Canady, Jerome

doi:10.1038/s41598-021-88451-w

Cited by 11 publications

(5 citation statements)

References 34 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Initially identified in our previous studies on carcinoma cell lines [38,43], inhibition of Ki67 expression and initiation of apoptosis by CHCP was replicated in this study on liposarcoma cells. We also observed a spike in the number of liposarcoma cells in the S/G2/M phase followed by initiation of cell death, consistent with our recent study on breast cancer cell lines [38].…”

Section: Discussionsupporting

confidence: 52%

Canady Cold Helios Plasma Reduces Soft Tissue Sarcoma Viability by Inhibiting Proliferation, Disrupting Cell Cycle, and Inducing Apoptosis: A Preliminary Report

Ly¹,

Cheng²,

Murthy³

et al. 2022

Molecules

Self Cite

View full text Add to dashboard Cite

Soft tissue sarcomas (STS) are a rare and highly heterogeneous group of solid tumors, originating from various types of connective tissue. Complete removal of STS by surgery is challenging due to the anatomical location of the tumor, which results in tumor recurrence. Additionally, current polychemotherapeutic regimens are highly toxic with no rational survival benefit. Cold atmospheric plasma (CAP) is a novel technology that has demonstrated immense cancer therapeutic potential. Canady Cold Helios Plasma (CHCP) is a device that sprays CAP along the surgical margins to eradicate residual cancer cells after tumor resection. This preliminary study was conducted in vitro prior to in vivo testing in a humanitarian compassionate use case study and an FDA-approved phase 1 clinical trial (IDE G190165). In this study, the authors evaluate the efficacy of CHCP across multiple STS cell lines. CHCP treatment reduced the viability of four different STS cell lines (i.e., fibrosarcoma, synovial sarcoma, rhabdomyosarcoma, and liposarcoma) in a dose-dependent manner by inhibiting proliferation, disrupting cell cycle, and inducing apoptosis-like cell death.

show abstract

Section: Discussionsupporting

confidence: 52%

Canady Cold Helios Plasma Reduces Soft Tissue Sarcoma Viability by Inhibiting Proliferation, Disrupting Cell Cycle, and Inducing Apoptosis: A Preliminary Report

Ly¹,

Cheng²,

Murthy³

et al. 2022

Molecules

Self Cite

View full text Add to dashboard Cite

show abstract

“…More recently, O. Jones et al demonstrated the interest of combining cold plasma with conventional FOLFIRINOX : a systemic chemotherapeutics used to treat CCA. They show that FOLFIRINOX can be administered at lower doses to reduce its toxicity burden while cold plasma is combined to obtain the same therapeutic efficiency [33].…”

Section: I23 Cold Plasma Applied To Ccamentioning

confidence: 99%

Cold plasma endoscopy applied to biliary ducts: feasibility risk assessment on human-like and porcine models for the treatment of cholangiocarcinoma

Decauchy

Pavy

Camus

et al. 2022

J. Phys. D: Appl. Phys.

View full text Add to dashboard Cite

For the past twelve years, cold plasmas (i.e., weakly ionized gas) have been positioned as a breakthrough technology for treating cancer thanks to their antitumor properties. The innovation of ad hoc plasma sources and personalized protocols appears crucial to treat cancers with a very poor prognosis. This is the case for cholangiocarcinoma (CCA), a biliary tract cancer, whose treatment with cold plasma is envisioned but requires the innovation of catheters and endoscopic devices for local therapies. Before conducting clinical trials, the performances and limitations of cold plasma endoscopy must be evaluated in terms of safety for both the patient and clinician as well as in terms of therapeutic efficacy. These objectives are pursued in the present work, in which a transferred plasma catheter is used, powered by 8-10 kV, 1-2 µs wide, 5-10 kHz repeated pulses, allowing guided streamers to be transferred over lengths of at least 2 m. In a first step, the catheter is utilized without the duodenoscope and directly inserted into an artificial model reproducing the topography and the electrical response of the biliary tree. This model allows to validate the technical feasibility of the technology and to demonstrate the absence of electrical and thermal risks. Indeed, the voltage and current deposited are as low as 3.98 V and 1.19 mA (RMS values) respectively, while the temperature locally increases from 23°C to 27 °C. In a second step, the catheter is inserted into the duodenoscope, the whole being applied to a porcine anatomical model. After passing through the esophagus, stomach and duodenum, the distal part of the duodenoscope is placed at the entrance to the papilla so that the catheter can easily enter the choledoc and then the common bile duct. Interestingly, the electrical power values deposited are of the order of 100 mW especially because the current values are at least 10 times higher. These more elevated values of electrical parameters but also electromagnetic effects are discussed considering physical aspects like eddy currents. The absence of electrical and thermal risks is demonstrated and consolidated by the IEC standards for medical devices. In a third time, we demonstrate that the cold plasma catheter can induce antitumor effects on in vitro experimental models of human CCA. The methodology proposed in this article validates the relevance of cold plasma endoscopy as a potential local treatment for cholangiocarcinoma and allows bridging cognitive and patient-oriented research.

show abstract

“…The advantages of underwater bone grinding during the operation of the plasma scalpel include effective washing of the operation cavity and further reduction of the temperature during the operation [ 56 , 57 ];…”

Section: Medical Applications Of Plasma Scalpelsmentioning

confidence: 99%

Plasma Scalpels: Devices, Diagnostics, and Applications

Xiao

Liu

He³

et al. 2022

Biomedicines

View full text Add to dashboard Cite

The plasma scalpel is an application of gas discharges in electrosurgery. This paper introduces the device structure and physicochemical parameters of the two types of plasma scalpels, namely, a single-electrode Ar discharge device (argon plasma coagulation) and a two-electrode discharge device in normal saline. The diagnostic methods, including the voltage and current characteristics, optical emission spectroscopy, electron spin resonance, and high-speed imaging, are introduced to determine the critical process parameters, such as the plasma power, the gas temperature, the electron density, and the density of active species, and study the ignition dynamics of the plasma discharges in water. The efficacy of the plasma scalpel is mainly based on the physical effects of the electric current and electric field, in addition to the chemical effects of high-density energetic electrons and reactive species. These two effects can be adjusted separately to increase the treatment efficacy of the plasma scalpel. Specific guidance on further improvements of the plasma scalpel devices is also provided.

show abstract

The synergistic effect of Canady Helios cold atmospheric plasma and a FOLFIRINOX regimen for the treatment of cholangiocarcinoma in vitro

Cited by 11 publications

References 34 publications

Canady Cold Helios Plasma Reduces Soft Tissue Sarcoma Viability by Inhibiting Proliferation, Disrupting Cell Cycle, and Inducing Apoptosis: A Preliminary Report

Canady Cold Helios Plasma Reduces Soft Tissue Sarcoma Viability by Inhibiting Proliferation, Disrupting Cell Cycle, and Inducing Apoptosis: A Preliminary Report

Cold plasma endoscopy applied to biliary ducts: feasibility risk assessment on human-like and porcine models for the treatment of cholangiocarcinoma

Plasma Scalpels: Devices, Diagnostics, and Applications

Contact Info

Product

Resources

About