The Synergistic Effect of Cationic Moieties and GRGDSF‐Peptides in Hydrogels on Neural Stem Cell Behavior

Abstract

This article reports the behavior of embryonic neural stem cells on a hydrogel that combines cationic, non-specific cell adhesion motifs with glycine-arginine-glycine-aspartic acid-serine-phenylalanine (GRGDSF)-peptides as specific cell adhesion moieties. Therefore, three hydrogels are prepared by free radical polymerization that contains either a GRGDSF-peptide residue (P1), amino ethylmethacrylate as a cationic residue (P2), or a combination of both motifs (P3). For each gel, cross linker concentrations of 8 mol% is used to have a comparable gel stiffness of 8-9 kPa. The cell experiments indicate a synergistic effect of the non-specific, cationic residues, and the specific GRGDSF-peptides on embryonic neural stem cell behavior that is especially pronounced in the cell adhesion experiments by more than doubling the number of cells after 72 h when comparing P3 with P2 and is less pronounced in the proliferation and differentiation experiments.

The Extracellular Matrix Proteins Tenascin-C and Tenascin-R Retard Oligodendrocyte Precursor Maturation and Myelin Regeneration in a Cuprizone-Induced Long-Term Demyelination Animal Model

“…Cells were stained immunocytochemically as previously described [ 34 , 35 ]. Medium from primary oligodendrocytes was removed and then the cells were washed twice with PBS and subsequently fixed with 4% ( w/v ) paraformaldehyde (PFA) (Cat.…”

The guanine nucleotide exchange factor Vav3 modulates oligodendrocyte precursor differentiation and supports remyelination in white matter lesions

“…Cells were stained immunocytochemically as previously described (Czopka, von Holst, ffrench‐Constant, & Faissner, ; Kandasamy, Roll, Langenstroth, Brustle, & Faissner, ; Sallouh et al, ). After removal of the culture medium from primary OLs, cells were washed once with KRH/A (Krebs‐Ringer‐HEPES buffer pH 7.3 + 0.1% [w/v] BSA) and primary antibodies for extracellular or membrane bound epitopes (O4) were diluted in KRH/A and incubated with the cells for 30 min at RT.…”

“…Cells were stained immunocytochemically as previously described (Czopka, von Holst, ffrench-Constant, & Faissner, 2010;Kandasamy, Roll, Langenstroth, Brustle, & Faissner, 2017;Sallouh et al, 2017).…”

Hydrogels Derivatized With Cationic Moieties or Functional Peptides as Efficient Supports for Neural Stem Cells

“…While cationic charges, substrate modulus and peptides on their own are promising tools to influence the behavior of NSCs, they are very often not combined in a single hydrogel (Jaiswal et al, 2013;Macková et al, 2016). Therefore, Sallouh et al (2017) investigated the effect of both the cationic charge and the peptide sequence GRGDSF in a cross-linked hydrogel system based on poly(acrylamide). In total the effect of three different hydrogels, namely a neutral gel bearing the peptide GRGDSF, a gel with cationic charges based on 2-aminoethylmethacrylate and a gel bearing both cationic charges and the peptide GRGDSF, on the behavior of NSCs were analyzed (see Figure 2).…”