The synergistic effect of trehalose and low concentrations of cryoprotectants can improve post-thaw ram sperm parameters

Öztürk, Ali Erdem; Bodu, Mustafa; Bucak, Mustafa Numan; Ağır, Vahit; Özcan, Ayşe; Keskin, Nazan; İli, Pınar; Topraggaleh, Tohid Rezaei; Sidal, Hümeyra; Başpınar, Nuri; Dursun, Şükrü

doi:10.1016/j.cryobiol.2020.03.008

Cited by 28 publications

(26 citation statements)

References 61 publications

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“…In the present study, the addition of trehalose resulted in protection for the sperm ultrastructural morphology, showing a correlation with the previous study. 53 We observed that the 5% ethylene glycol and 1.5% ethylene glycol +100 mM trehalose provided the highest protection for the ultrastructural morphology of cryopreserved ram spermatozoa. Thus, it can be postulated that 1.5% ethylene glycol could play a role as a cocryoprotectant with 100 mM trehalose on cryopreserved ram spermatozoa.…”

Section: Effects Of Cryoprotectants On Frozen-thawed Ram Spermatozoa 443mentioning

confidence: 71%

“…In this sense, electron microscopy techniques have the advantage of examining ultrastructural changes of spermatozoa after freezing and thawing processes. Ozturk et al 53 showed that the postthaw sperm parameters (acrosome integrity, DNA fragmentation, and DNA Integrity) could be improved by the supplementation of the semen extender with trehalose, which is shown in Table 2.…”

Section: Effects Of Cryoprotectants On Frozen-thawed Ram Spermatozoa 443mentioning

confidence: 99%

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Cryopreservation Effects on Ram Sperm Ultrastructure

Keskin

Erdogan

Bucak

et al. 2020

Biopreservation and Biobanking

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Cryoprotectants are known to have protective effects against cryodamage to spermatozoa. In this study, the cryoprotective effects of two cryoprotectants (glycerol, ethylene glycol) and cryoprotectants/trehalose combinations on frozen-thawed ram spermatozoa were investigated at the ultrastructural level. For this purpose, ejaculates collected from Konya Merino rams were pooled and diluted with a tris-based extender containing additives, including 5% glycerol, 3% glycerol +60 mM trehalose, 1.5% glycerol +100 mM trehalose, 5% ethylene glycol, 3% ethylene glycol +60 mM trehalose, and 1.5% ethylene glycol +100 mM trehalose. They were all cooled to 5°C and then frozen in 0.25 mL French straws in liquid nitrogen. The samples were thawed at 37°C and centrifuged to remove the diluents. Then, they were processed using a scanning transmission electron microscope. In the statistical analysis, the number of ultrastructurally cryodamaged and intact spermatozoa were counted in longitudinal and transverse ultrathin sections in all groups by electron microscopic examination. The amount of intact spermatozoa in the groups containing 5% ethylene glycol and 1.5% ethylene glycol +100 mM trehalose was found to be higher than other groups (p < 0.05). As a result, it was suggested that the groups of 5% ethylene glycol and 1.5% ethylene glycol +100 mM trehalose provided the highest protection for the ultrastructural morphology of frozen-thawed Konya Merino ram spermatozoa among the groups.

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Section: Effects Of Cryoprotectants On Frozen-thawed Ram Spermatozoa 443mentioning

confidence: 71%

Section: Effects Of Cryoprotectants On Frozen-thawed Ram Spermatozoa 443mentioning

confidence: 99%

Cryopreservation Effects on Ram Sperm Ultrastructure

Keskin

Erdogan

Bucak

et al. 2020

Biopreservation and Biobanking

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“…Trehalose also shows a direct interaction with phospholipid polar head groups, and because of this interaction, it stabilizes membrane phospholipids. However, the cracks of the inner and outer layer membrane (non‐stabilized cell membrane) cause cell dehydration and osmotic imbalance 16,23 …”

Section: Introductionmentioning

confidence: 99%

Combination of fetuin and trehalose in presence of low glycerol has beneficial effects on freeze‐thawed ram spermatozoa

et al. 2021

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Background Freeze‐thawing process negatively affects ram spermatozoa in terms of sperm quality, DNA integrity and antioxidant defence system. Thus, antioxidant supplementation of spermatozoa during freeze‐thawing is suggested to improve sperm parameters. Objectives The aim of this study was to determine the effects of fetuin and trehalose added into ram semen extender on sperm parameters, antioxidant parameters, antioxidant‐related gene expressions and DNA integrity during the freeze‐thawing process, in low glycerol concentration. Methods Semen samples collected from six mature rams were pooled and splitted into equal aliquots and diluted with a tris‐based extender containing different concentrations of glycerol (G5; %5 and G3; %3), fetuin (F; 2.5, 5 and 15 mg/mL) and trehalose (60 mm) as eight groups (G5F0, G5F2.5, G5F5, G5F15, G3F0, G3F2.5, G3F5 and G3F15). Results G3F5 group resulted in the highest motility, mitochondrial activity and viability and the lowest DNA fragmentation and DNA damage (p < 0.05). Also, G3F0 displayed considerably more cryoprotective effect compared with G5F0 group (p < 0.05) in terms of motility, mitochondrial activity and viability rates. Lipid peroxidation levels decreased in G5F5 group compared with G5F0 group (p < 0.05). The levels of total glutathione increased in G3F2.5 group (p < 0.05) in comparison with the G5F0 group. NQO1 gene levels were upregulated approximately twofold in G5F5, G5F15, G3F2.5, G3F5 and G3F15 groups compared with G5F0 group (p < 0.05). The levels of GCLC gene were approximately twofold higher in G3F0, G3F2.5, G3F5 and G3F15 groups compared with G5F0 group (p < 0.05). GSTP1 gene levels were significantly higher with different levels in all treatment groups except for G5F2.5 and G3F0 groups in comparison with G5F0 group (p < 0.05). Conclusions Co‐supplementation of tris‐based extender having low glycerol (3%) with trehalose and fetuin to enhance the quality of ram spermatozoa after freeze‐thawing process is recommended.

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“…Therefore, membrane injuries could happen, and with this, a decrease in sperm motility and viability. The harmful effects of osmotic stress due to the high concentrations of trehalose supplemented to the freezing extender on post-thawing semen quality has also been reported in ram (Aisen et al, 2002;Öztürk et al, 2020), boar (Hu et al, 2009), and chicken (Thananurak et al, 2016) semen.…”

Section: Supplementation Of Trehalose and Gsh To The Semen Freezing Extendermentioning

confidence: 91%

“…Many authors (Najafi et al, 2013;Iqbal et al, 2018;Öztürk et al, 2020), showed that trehalose has a synergic effect with glycerol when they are found at specific concentrations in the freezing extender, and this may affect the post-thawing sperm quality and may depend on the extender type. Furthermore, others have confirmed that glycerol could be replaced by trehalose in the semen extender to give equivalent post-thawing semen quality (Athurupana et al, 2015).…”

Section: Supplementation Of Trehalose and Gsh To The Semen Freezing Extendermentioning

confidence: 99%

Cellular Basis and Optimization of Cryopreservation Survival of Bull Sperm = Base celular y optimización de la supervivencia de la criopreservación de esperma de toro

Albu-Mohammed¹

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The synergistic effect of trehalose and low concentrations of cryoprotectants can improve post-thaw ram sperm parameters

Cited by 28 publications

References 61 publications

Cryopreservation Effects on Ram Sperm Ultrastructure

Cryopreservation Effects on Ram Sperm Ultrastructure

Combination of fetuin and trehalose in presence of low glycerol has beneficial effects on freeze‐thawed ram spermatozoa

Cellular Basis and Optimization of Cryopreservation Survival of Bull Sperm = Base celular y optimización de la supervivencia de la criopreservación de esperma de toro

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