The synergistic effects of graphene-contained 3D-printed calcium silicate/poly-ε-caprolactone scaffolds promote FGFR-induced osteogenic/angiogenic differentiation of mesenchymal stem cells

Lin, Yu‐Hung; Chuang, Tsan-Yu; Chiang, Wei‐Hung; Chen, I‐Wen Peter; Wang, Kan; Shie, Ming‐You; Chen, Yiwen

doi:10.1016/j.msec.2019.109887

Cited by 62 publications

(59 citation statements)

References 59 publications

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“…The CS matrices were produced using the thermal pressing method 63 . In a previous study 64 , we demonstrated that the ratio of PCL/CS = 50/50 had better mechanical properties and cell behaviours. First, 47.5 mg PCL was placed in a 150°C oven for 2 h. Then, 47.5 mg CS powder and 5 mg of the exfoliated WS 2 thin sheets were suspended in 5 mL alcohol and dropped into the PCL.…”

Section: Fabrication Of the Ws 2 /Pcl/cs Composite Scaffoldsmentioning

confidence: 78%

Synthesis of high-quality monolayer tungsten disulfide with chlorophylls and its application for enhancing bone regeneration

et al. 2020

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Due to the population explosion of the 21st century, nearly one billion people are over 64 years of age and bone fracture is one of the most frequent problems facing both sexes because of osteoporosis. However, difficulty in enhancing bone regeneration to repair bone fracture poses challenges and thus, a two-dimensional monolayer material (i.e. tungsten disulfide (WS2)) could be one of the candidates offering a possible solution to the problem. Here, we prepare high-quality monolayer WS2 thin sheets in a large quantity with the assistance of extracted chlorophyll molecules, the natural pigment used in photosynthesis, via a liquid-phase exfoliation method. Then, the exfoliated WS2 sheets were mixed with polycaprolactone (PCL)/calcium silicate (CS) to form a biocompatible WS2-based composite. The in vivo experiments show that the bone regeneration of the WS2-based composite was 120% superior to commercially available mineral trioxide aggregate (MTA) bone cement. Moreover, the mechanical properties of the WS2-based composite exhibited ~300% enhancement over PCL/CS, which is one of the most commonly used bone regeneration materials. Our findings highlight the prospects for the composite of WS2 towards the improvement of bone regeneration applications.

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Section: Fabrication Of the Ws 2 /Pcl/cs Composite Scaffoldsmentioning

confidence: 78%

Synthesis of high-quality monolayer tungsten disulfide with chlorophylls and its application for enhancing bone regeneration

et al. 2020

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“…Due to technological advancements in science, we have a better understanding of the healing physiology of bone defects at a molecular level. Researchers have successfully identified a number of key molecules which have a crucial role to play in the healing process of bone defects [18]. Of these molecules, researchers largely focused on bone morphogenetic proteins (BMPs), which were known to be a potent osteoinductive factor.…”

Section: Introductionmentioning

confidence: 99%

Effect of Bone Morphogenic Protein-2-Loaded Mesoporous Strontium Substitution Calcium Silicate/Recycled Fish Gelatin 3D Cell-Laden Scaffold for Bone Tissue Engineering

Wang

Liu

et al. 2020

Processes

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Bone has a complex hierarchical structure with the capability of self-regeneration. In the case of critical-sized defects, the regeneration capabilities of normal bones are severely impaired, thus causing non-union healing of bones. Therefore, bone tissue engineering has since emerged to solve problems relating to critical-sized bone defects. Amongst the many biomaterials available on the market, calcium silicate-based (CS) cements have garnered huge interest due to their versatility and good bioactivity. In the recent decade, scientists have attempted to modify or functionalize CS cement in order to enhance the bioactivity of CS. Reports have been made that the addition of mesoporous nanoparticles onto scaffolds could enhance the bone regenerative capabilities of scaffolds. For this study, the main objective was to reuse gelatin from fish wastes and use it to combine with bone morphogenetic protein (BMP)-2 and Sr-doped CS scaffolds to create a novel BMP-2-loaded, hydrogel-based mesoporous SrCS scaffold (FGSrB) and to evaluate for its composition and mechanical strength. From this study, it was shown that such a novel scaffold could be fabricated without affecting the structural properties of FGSr. In addition, it was proven that FGSrB could be used for drug delivery to allow stable localized drug release. Such modifications were found to enhance cellular proliferation, thus leading to enhanced secretion of alkaline phosphatase and calcium. The above results showed that such a modification could be used as a potential alternative for future bone tissue engineering research.

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“…Such 3D printing technology has been widely applied to bone tissue engineering as it gives us the flexibility to customize personalized grafts according to one's need quickly and with reproducibility [7][8][9]. Certain parameters are known to be characteristics and properties of scaffolds, such as porosity, pore size and shape, sinter temperature, crystal distribution and phase composition [10][11][12]. It is worth noting that porosity is a critical factor and a uniform porosity was shown to allow good bio-resorption, biodegradation and a stable release rate of ions, thus leading to subsequently enhanced bone regeneration, bone remodeling, angiogenesis and vascularization [13].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Release of Vascular Endothelial Growth Factor from 3D-Printed Poly-ε-Caprolactone/Hydroxyapatite/Calcium Sulfate Scaffold with Enhanced Osteogenic Capacity

Chen

Wang

et al. 2020

Polymers

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Vascular endothelial growth factor (VEGF) is one of the most crucial growth factors and an assistant for the adjustment of bone regeneration. In this study, a 3D scaffold is fabricated using the method of fused deposition modeling. Such a fabricated method allows us to fabricate scaffolds with consistent pore sizes, which could promote cellular ingrowth into scaffolds. Therefore, we drafted a plan to accelerate bone regeneration via VEGF released from the hydroxyapatite/calcium sulfate (HACS) scaffold. Herein, HACS will gradually degrade and provide a suitable environment for cell growth and differentiation. In addition, HACS scaffolds have higher mechanical properties and drug release compared with HA scaffolds. The drug release profile of the VEGF-loaded scaffolds showed that VEGF could be loaded and released in a stable manner. Furthermore, initial results showed that VEGF-loaded scaffolds could significantly enhance the proliferation of human mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells (HUVEC). In addition, angiogenic- and osteogenic-related proteins were substantially increased in the HACS/VEGF group. Moreover, in vivo results revealed that HACS/VEGF improved the regeneration of the rabbit’s femur bone defect, and VEGF loading improved bone tissue regeneration and remineralization after implantation for 8 weeks. All these results strongly imply that the strategy of VEGF loading onto scaffolds could be a potential candidate for future bone tissue engineering.

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The synergistic effects of graphene-contained 3D-printed calcium silicate/poly-ε-caprolactone scaffolds promote FGFR-induced osteogenic/angiogenic differentiation of mesenchymal stem cells

Cited by 62 publications

References 59 publications

Synthesis of high-quality monolayer tungsten disulfide with chlorophylls and its application for enhancing bone regeneration

Synthesis of high-quality monolayer tungsten disulfide with chlorophylls and its application for enhancing bone regeneration

Effect of Bone Morphogenic Protein-2-Loaded Mesoporous Strontium Substitution Calcium Silicate/Recycled Fish Gelatin 3D Cell-Laden Scaffold for Bone Tissue Engineering

Assessment of the Release of Vascular Endothelial Growth Factor from 3D-Printed Poly-ε-Caprolactone/Hydroxyapatite/Calcium Sulfate Scaffold with Enhanced Osteogenic Capacity

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