The synergistic effects of volatile constituents of  Ocimum basilicum  against foodborne pathogens

Baldim, João L.; Silveira, Juliana Grell Fernandes; Almeida, Anelique Pinto; Carvalho, Patrícia Lunardelli Negreiros de; Rosa, Welton; Schripsema, Jan; Chagas‐Paula, Daniela Aparecida; Soares, Marisi G.; Luiz, Jaine Honorata Hortolan

doi:10.1016/j.indcrop.2017.12.016

Cited by 27 publications

(10 citation statements)

References 37 publications

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“…25 In fact, the use of MSA in metabolomics allows a systematic investigation of highly complex matrices of phytochemicals and the association of each component to the biological activity in a holistic approach. 26,27 The essential oils extracted from leaves of G. macrophylla collected from populations I (SP) and II (CB) displayed chemical variations among them, resulting in unique values of EC 50 . In order to identify the influence of the components to antileishmanial activity two different groups were created: A (EC 50 values fewer than 13.6 µg mL -1 ), and B (EC 50 values higher than 13.6 µg mL -1 ), comparing the obtained EC 50 values to that calculated for positive control miltefosine (EC 50 = 5.9 ± 1.8 µg mL -1 ).…”

Section: Resultsmentioning

confidence: 99%

A Comparative Study on Chemical Composition, Antileishmanial and Cytotoxic Activities of the Essential Oils from Leaves of Guarea macrophylla (Meliaceae) from Two Different Regions of São Paulo State, Brazil, Using Multivariate Statistical Analysis

Oliveira¹,

Martins²,

Soares³

et al. 2019

J. Braz. Chem. Soc.

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Meliaceae representatives are economically important in several aspects including the production of highly prized woods (mahogany, cedar, etc.), constituents for cosmetics, and insecticides. The present study aimed to verify the chemical composition as well as leishmanicidal and cytotoxic potential of essential oils from leaves of two different populations of Guarea macrophylla collected at cities of São Paulo (population I) and Cubatão (population II), São Paulo State, Brazil. Chemically, the oils showed the predominance of sesquiterpenes: cis-β-guaiene, bicyclogermacrene, viridiflorol, and isolongifolan-7α-ol from population I and α-copaene, E-caryophyllene, cis-β-guaiene, and γ-amorphene from population II. In vitro antileishmanial activity against promastigote forms of Leishmania (L.) amazonensis of essential oils was evaluated and displayed 50% effective concentration (EC 50) values ranging from 11.8 to 20.5 µg mL-1. Furthermore, toxicity against peritoneal macrophages of BALB/c mice was observed, with 50% cytotoxic concentration (CC 50) ranging from 17.7 to > 100 µg mL-1. Multivariate statistical analysis revealed the influence of each constituent of the oils against L. amazonensis being 1,10-di-epi-cubenol, α-amorphene, E-caryophyllene, isopimara-7,15-diene, and β-elemene associated with the antileishmanial potential.

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Section: Resultsmentioning

confidence: 99%

A Comparative Study on Chemical Composition, Antileishmanial and Cytotoxic Activities of the Essential Oils from Leaves of Guarea macrophylla (Meliaceae) from Two Different Regions of São Paulo State, Brazil, Using Multivariate Statistical Analysis

Oliveira¹,

Martins²,

Soares³

et al. 2019

J. Braz. Chem. Soc.

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“…The prominently enriched GO terms are demonstrated in Figures S3 and S4. Most of the 90 up regulated DEPs were mainly involved in small molecular metabolic process (15,16.67%), carboxylic acid metabolic process (10, 11.11%), oxoacid metabolic process (10, 11.11%), organic acid metabolic process (10, 11.11%), small molecule biosynthetic process (7, 7.78%) and cellular amino acid metabolic process (6, 6.67%). Certain other up regulated DEPs enriched the metabolism of carbohydrate and amino acid derivatives.…”

Section: Go Annotation Analysis Of the Depsmentioning

confidence: 99%

“…Additionally, ribosome related components were also well enriched with down regulated DEPs. The molecular functions of these down regulated DEPs included the structural constituent of ribosome (15,11.11%), structural molecule activity (15,11.11%), RNA binding (10, 7.41%) and others such as transferase activity ( Figure S4).…”

Section: Go Annotation Analysis Of the Depsmentioning

confidence: 99%

“…Nonetheless, this candidacy is compromised by the expensiveness of these natural substances due to their generally low yields. However, exploring their mode of actions is valuable to the development of novel alternatives.The multicomponent EOs attribute their mode of action to various responsible components [14,15] rather than a simple and sole-mechanism action of an individual component. This wide mode action is an advantage of EOs over synthetic fungicides, but at the same time, it complicates the elucidation of the antifungal mechanisms of EOs.…”

mentioning

confidence: 99%

“…The multicomponent EOs attribute their mode of action to various responsible components [14,15] rather than a simple and sole-mechanism action of an individual component. This wide mode action is an advantage of EOs over synthetic fungicides, but at the same time, it complicates the elucidation of the antifungal mechanisms of EOs.…”

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confidence: 99%

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Cuminal Inhibits Trichothecium roseum Growth by Triggering Cell Starvation: Transcriptome and Proteome Analysis

Zhang

et al. 2020

Microorganisms

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Trichothecium roseum is a harmful postharvest fungus causing serious damage, together with the secretion of insidious mycotoxins, on apples, melons, and other important fruits. Cuminal, a predominant component of Cuminum cyminum essential oil has proven to successfully inhibit the growth of T. roseum in vitro and in vivo. Electron microscopic observations revealed cuminal exposure impaired the fungal morphology and ultrastructure, particularly the plasmalemma. Transcriptome and proteome analysis was used to investigate the responses of T. roseum to exposure of cuminal. In total, 2825 differentially expressed transcripts (1516 up and 1309 down) and 225 differentially expressed proteins (90 up and 135 down) were determined. Overall, notable parts of these differentially expressed genes functionally belong to subcellular localities of the membrane system and cytosol, along with ribosomes, mitochondria and peroxisomes. According to the localization analysis and the biological annotation of these genes, carbohydrate and lipids metabolism, redox homeostasis, and asexual reproduction were among the most enriched gene ontology (GO) terms. Biological pathway enrichment analysis showed that lipids and amino acid degradation, ATP-binding cassette transporters, membrane reconstitution, mRNA surveillance pathway and peroxisome were elevated, whereas secondary metabolite biosynthesis, cell cycle, and glycolysis/gluconeogenesis were down regulated. Further integrated omics analysis showed that cuminal exposure first impaired the polarity of the cytoplasmic membrane and then triggered the reconstitution and dysfunction of fungal plasmalemma, resulting in handicapped nutrient procurement of the cells. Consequently, fungal cells showed starvation stress with limited carbohydrate metabolism, resulting a metabolic shift to catabolism of the cell's own components in response to the stress. Additionally, these predicaments brought about oxidative stress, which, in collaboration with the starvation, damaged certain critical organelles such as mitochondria. Such degeneration, accompanied by energy deficiency, suppressed the biosynthesis of essential proteins and inhibited fungal growth.fungicides to be used for postharvest treatment, reduced efficacy due to the emergence of pathogen resistance to these chemicals, and the growing public concerns over chemical residues in food and the environment have necessitated the seeking of alternative solutions to control the fungal spoilage and to guarantee food safety and consumer health [8].Essential oils (EOs), generally recognized as safe by the US Food and Drug Administration [9], are natural substances with established antimicrobial activities [10,11] and are a candidate instrument for controlling postharvest disease [12,13]. Nonetheless, this candidacy is compromised by the expensiveness of these natural substances due to their generally low yields. However, exploring their mode of actions is valuable to the development of novel alternatives.The multicomponent EOs attribute their mode of act...

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Essential oils and their blends: mechanism of antibacterial activity and antibiofilm potential on food-grade maize starch packaging films

Kaur,

Sharma,

Kalia

et al. 2024

Int Microbiol

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The synergistic effects of volatile constituents of Ocimum basilicum against foodborne pathogens

Cited by 27 publications

References 37 publications

A Comparative Study on Chemical Composition, Antileishmanial and Cytotoxic Activities of the Essential Oils from Leaves of Guarea macrophylla (Meliaceae) from Two Different Regions of São Paulo State, Brazil, Using Multivariate Statistical Analysis

A Comparative Study on Chemical Composition, Antileishmanial and Cytotoxic Activities of the Essential Oils from Leaves of Guarea macrophylla (Meliaceae) from Two Different Regions of São Paulo State, Brazil, Using Multivariate Statistical Analysis

Cuminal Inhibits Trichothecium roseum Growth by Triggering Cell Starvation: Transcriptome and Proteome Analysis

Essential oils and their blends: mechanism of antibacterial activity and antibiofilm potential on food-grade maize starch packaging films

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