The Synergy of Topographical Micropatterning and Ta|TaCu Bilayered Thin Film on Titanium Implants Enables Dual‐Functions of Enhanced Osteogenesis and Anti‐Infection

Abstract

Poor osteogenesis and implant‐associated infection are the two leading causes of failure for dental and orthopedic implants. Surface design with enhanced osteogenesis often fails in antibacterial activity, or vice versa. Herein, a surface design strategy, which overcomes this trade‐off via the synergistic effects of topographical micropatterning and a bilayered nanostructured metallic thin film is presented. A specific microgrooved pattern is fabricated on the titanium surface, followed by sequential deposition of a nanostructured copper (Cu)‐containing tantalum (Ta) (TaCu) layer and a pure Ta cap layer. The microgrooved patterns coupled with the nanorough Ta cap layer shows strong contact guidance to preosteoblasts and significantly enhances the osteogenic differentiation in vitro, while the controlled local sustained release of Cu ions is responsible for high antibacterial activity. Importantly, rat calvarial defect models in vivo further confirm that the synergy of microgrooved patterns and the Ta|TaCu bilayered thin film on titanium surface could effectively promote bone regeneration. The present effective and versatile surface design strategy provides significant insight into intelligent surface engineering that can control biological response at the site of healing in dental and orthopedic implants.

Evaluation of the immunomodulatory effects of cobalt, copper and magnesium ions in a pro inflammatory environment

“…This combination has already been used for bone regeneration applications, where the combination of surface topographies with Cu 2+ release improved osteogenesis. However, this approach has not been explored in the context of immunomodulation to the best of our knowledge 45 .…”

Developing a Versatile Multiscale Therapeutic Platform for Osteosarcoma Synergistic Photothermo-Chemotherapy with Effective Osteogenicity and Antibacterial Capability

“…Relying on the design of multiscale micro-nanostructures on implant surfaces for controlling cell reprogramming to promote bone regeneration constitutes a new direction in the development of next-generation orthopedic implants. − Multiscale micro-nanostructures increase the bone-implant contact area, promote early cell responses, and encourage the ingrowth of new bone that ensures the bonding strength of implants. , These effects are clinically important for improving the success rate and durability of implants. However, such multiscale micro-nanostructures have no ability to treat osteosarcoma and bacterial infection.…”

Emerging modulators for osteogenic differentiation: a combination of chemical and topographical cues for bone microenvironment engineering

“…The fabrication of microgrooved Ti substrates followed by the deposition of a nanostructured copper-containing tantalum layer and a pure tantalum cap layer induced the alignment of pre-osteoblasts and the anisotropic assembly of their stress actin fibers through strong contact guidance while enabling cell adhesion and anti–infection properties. 53…”

“…The fabrication of microgrooved Ti substrates followed by the deposition of a nanostructured copper-containing tantalum layer and a pure tantalum cap layer induced the alignment of pre-osteoblasts and the anisotropic assembly of their stress actin fibers through strong contact guidance while enabling cell adhesion and anti-infection properties. 53 Moreover, taking into consideration the importance of the native ECM, namely its composition and topography in the behavior of the bone resident cells, researchers combined wrinkled polydimethylsiloxane (PDMS) scaffolds of different sizes and cell-derived matrices (CDM). 54 After 10-days of culture with human fibroblasts, the subsequent decellularization of the scaffolds was used to study its impact in controlling the behaviour of MSCs.…”