The Synovial Lining and Synovial Fluid Properties after  Joint Arthroplasty

Kung, Michael S.; Markantonis, John; Nelson, Scott D.; Campbell, Patricia A.

doi:10.3390/lubricants3020394

Cited by 29 publications

(16 citation statements)

References 54 publications

(65 reference statements)

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“…In RA, inflammation and cellular infiltration cause hyperplasia of the synovial tissue leading to the pannus formation. The pannus is a tissue composed of MLS, FLS, leucocytes, plasma cells and mastocytes that mediates cartilage damage and bone erosion [7][8][9][10] .…”

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confidence: 99%

Identification of putative master regulators in rheumatoid arthritis synovial fibroblasts using gene expression data and network inference

Zerrouk

Miagoux

Dispot

et al. 2020

Sci Rep

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Rheumatoid arthritis (RA) is a systemic autoimmune disease that affects the synovial joints of the body. Rheumatoid arthritis fibroblast-like synoviocytes (RA FLS) are central players in the disease pathogenesis, as they are involved in the secretion of cytokines and proteolytic enzymes, exhibit invasive traits, high rate of self-proliferation and an apoptosis-resistant phenotype. We aim at characterizing transcription factors (TFs) that are master regulators in RA FLS and could potentially explain phenotypic traits. We make use of differentially expressed genes in synovial tissue from patients suffering from RA and osteoarthritis (OA) to infer a TF co-regulatory network, using dedicated software. The co-regulatory network serves as a reference to analyze microarray and single-cell RNA-seq data from isolated RA FLS. We identified five master regulators specific to RA FLS, namely BATF, POU2AF1, STAT1, LEF1 and IRF4. TF activity of the identified master regulators was also estimated with the use of two additional, independent software. The identified TFs contribute to the regulation of inflammation, proliferation and apoptosis, as indicated by the comparison of their differentially expressed target genes with hallmark molecular signatures derived from the Molecular Signatures Database (MSigDB). Our results show that TFs influence could be used to identify putative master regulators of phenotypic traits and suggest novel, druggable targets for experimental validation.

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mentioning

confidence: 99%

Identification of putative master regulators in rheumatoid arthritis synovial fibroblasts using gene expression data and network inference

Zerrouk

Miagoux

Dispot

et al. 2020

Sci Rep

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“…Beyond filling the space in the joint cavity and absorbing shock, adipose tissues also secrete cytokines and adipokines ( 29 , 30 ) and are therefore potent immune-modulators. It is also believed that the infrapatellar fat pad engages in intimate crosstalk with the synovial membrane, a specialized connective tissue that lines the inner surface of the joint capsule ( 31 ). The synovial membrane consists of three layers: The intimal lining layer is found closest to the joint cavity and consists mainly of macrophages (“type A cells”) and fibroblasts (“type B cells”) that show low degrees of cell division ( 31 ).…”

Section: Macro- and Microanatomy Of The Knee Jointmentioning

confidence: 99%

Monocytes, Macrophages, and Their Potential Niches in Synovial Joints – Therapeutic Targets in Post-Traumatic Osteoarthritis?

et al. 2021

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Synovial joints are complex structures that enable normal locomotion. Following injury, they undergo a series of changes, including a prevalent inflammatory response. This increases the risk for development of osteoarthritis (OA), the most common joint disorder. In healthy joints, macrophages are the predominant immune cells. They regulate bone turnover, constantly scavenge debris from the joint cavity and, together with synovial fibroblasts, form a protective barrier. Macrophages thus work in concert with the non-hematopoietic stroma. In turn, the stroma provides a scaffold as well as molecular signals for macrophage survival and functional imprinting: “a macrophage niche”. These intricate cellular interactions are susceptible to perturbations like those induced by joint injury. With this review, we explore how the concepts of local tissue niches apply to synovial joints. We introduce the joint micro-anatomy and cellular players, and discuss their potential interactions in healthy joints, with an emphasis on molecular cues underlying their crosstalk and relevance to joint functionality. We then consider how these interactions are perturbed by joint injury and how they may contribute to OA pathogenesis. We conclude by discussing how understanding these changes might help identify novel therapeutic avenues with the potential of restoring joint function and reducing post-traumatic OA risk.

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“…During loading and movement of the femur, synovial liquid is squeezed out into the natural joints. Kung et al [1] describe the importance of preserving the integrity of joint including the synovial lining and natural lubricating fluid properties for future successful implant survivorship.…”

Section: Introductionmentioning

confidence: 99%

Harsh Sliding Wear of a Zirconia Ball against a-C:H Coated CoCrMo Disc in Hyaluronic Gel

2020

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The a-C:H (amorphous carbon-hydrogen) films belong to the family of DLC (diamond-like carbon) coatings. The a-C:H coating was deposited on medical grade CoCrMo substrates by plasma-assisted chemical vapor deposition (PA-CVD) using benzene as gaseous precursor. Benzene offers an aromatic structure, which affects the a-C:H properties after plasma decomposition. A zirconia ball was sliding at two different frequencies, 50 Hz or 1Hz, against the uncoated and a-C:H coated CoCrMo. The frequency of 1 Hz is typical for human movement during fast walking. The harsh sliding conditions with a normal load of 100 N and 50 Hz frequency simulate extreme overloading of the biomedical sliding partners. It gives insight into the failure mechanisms. The wear tests were carried out in laboratory air (dry, RH: 15.6%) or using hyaluronic gel as lubricant. The hyaluronic gel acts as an effective intermediate medium. It adheres very well to both, a-C:H coating and zirconia. No wear was evident on the ZrO2 ball at 1 Hz and 100 N. Minor wear traces were observed on the a-C:H coating only. A wear coefficient of 0.16 × 10−6 mm3/N·m were calculated for a-C:H coated CoCrMo after ZrO2 ball sliding with 1 Hz and 100 N in hyaluronic gel. This is two orders of magnitude lower in comparison to dry sliding of ZrO2 ball against DLC coated CoCrMo with 1 Hz. The coefficient of friction (COF) remained below 0.09 until the hyaluronic gel starts to lose viscosity. This finding pronounces the importance of a proper homogeneous lubrication during operation of the biomedical joints. For extreme harsh tribological loading like sudden jumps of a patient with artificial joints, the application of an intermediate layer before a-C:H coating needs further evaluation.

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The Synovial Lining and Synovial Fluid Properties after Joint Arthroplasty

Cited by 29 publications

References 54 publications

Identification of putative master regulators in rheumatoid arthritis synovial fibroblasts using gene expression data and network inference

Identification of putative master regulators in rheumatoid arthritis synovial fibroblasts using gene expression data and network inference

Monocytes, Macrophages, and Their Potential Niches in Synovial Joints – Therapeutic Targets in Post-Traumatic Osteoarthritis?

Harsh Sliding Wear of a Zirconia Ball against a-C:H Coated CoCrMo Disc in Hyaluronic Gel

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