The synovitis of "non-inflammatory" orthopaedic arthropathies: a quantitative histological and immunohistochemical analysis

Peßler, Frank; Dai, Lei; Díaz-Torné, Cèsar; Gómez-Vaquero, Carmen; Me, Paessler; Dh, Zheng; Einhorn, Eugene; Range, Ursula; Scanzello, Carla R.; Hr, Schumacher

doi:10.1136/ard.2008.087775

Cited by 101 publications

(96 citation statements)

References 11 publications

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“…In response to injury of various types, including trauma, the synovial membrane rapidly becomes hyperplastic (2,3). It is commonly believed that synovial hyperplasia is sustained mainly by stromal cells including type B (fibroblast-like) synoviocytes, also called synovial fibroblasts, with infiltration of blood-borne inflammatory and immune cells particularly in inflammatory joint diseases such as rheumatoid arthritis (4).…”

Section: Conclusion Our Findings Provide the First Evidence Of The Ementioning

confidence: 99%

Functional mesenchymal stem cell niches in adult mouse knee joint synovium in vivo

Kurth¹,

Dell’Accio²,

Crouch³

et al. 2011

Arthritis & Rheumatism

180

174

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Objective. We previously reported that human synovium contains cells that, after culture expansion, display properties of mesenchymal stem cells (MSCs). The objective of this study was to identify MSCs in native synovium in vivo.Methods. To identify stem cells in the synovium in vivo, a double nucleoside analog cell-labeling scheme was used in a mouse model of joint-surface injury. For labeling of slow-cycling cells, mice received iododeoxyuridine (IdU) for 30 days, followed by a 40-day washout period. For labeling of cells that proliferate after injury, mice underwent knee surgery to produce an articular cartilage defect and received chlorodeoxyuridine (CIdU) for 4 days, starting at multiple time points after surgery. Unoperated and sham-operated joints served as controls. Knee joint paraffin sections were analyzed by double and triple immunostaining to detect nucleoside analogs, conventional MSC markers, and chondrocytelineage markers.Results. Long-term-retaining, slow-cycling IdUpositive cells were detected in the synovium. At 4 days and 8 days after injury, there was marked proliferation of IdU-positive cells, which costained for CIdU. IdUpositive cells were nonhematopoietic, nonendothelial stromal cells, were distinct from pericytes, and stained positive for MSC markers. MSCs were phenotypically heterogeneous and located in topographically distinct niches in the lining layer and the subsynovial tissue. Twelve days after injury, double nucleoside-labeled cells within synovium were embedded in cartilagespecific metachromatic extracellular matrix and costained positive for the chondrocyte-lineage markers Sox9 and type II collagen.Conclusion. Our findings provide the first evidence of the existence of resident MSCs in the knee joint synovium that undergo proliferation and chondrogenic differentiation following injury in vivo.

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Section: Conclusion Our Findings Provide the First Evidence Of The Ementioning

confidence: 99%

Functional mesenchymal stem cell niches in adult mouse knee joint synovium in vivo

Kurth¹,

Dell’Accio²,

Crouch³

et al. 2011

Arthritis & Rheumatism

180

174

View full text Add to dashboard Cite

show abstract

“…Although this grading system is the only established score [45], it is intended primarily to differentiate between OA and rheumatoid arthritis. More advanced immunohistochemical markers like Ki-67 or CD68 [38] might be necessary to define a more sensitive grading system for the synovium in patients with OA of the knee. Such a grading system might lead to better correlations of the histopathologic degree of synovitis and biomechanical properties of articular cartilage in the lateral compartment in patients with medial compartment OA.…”

Section: Discussionmentioning

confidence: 99%

Does Intraarticular Inflammation Predict Biomechanical Cartilage Properties?

Waldstein

Perino

Jawetz

et al. 2014

Clinical Orthopaedics &Amp; Related Research

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“…This is consistent with reports in established osteoarthritis patients and suggests that synovitis has important clinical implications at early, "pre-clinical" stages of disease. Other changes such as synovial lining hyperplasia and increased vascularity are also observed and often included in summed histologic scores of "inflammation" [9]. Whether these varying patterns of synovial histopathologic change reflect different molecular mechanisms, different stages of the inflammatory process, or have the same impact on disease progression and symptomatology, are important questions that remain.…”

Section: Histopathologic Analysis Of Synovitis In Oamentioning

confidence: 99%

Pathologic and Pathogenic Processes in Osteoarthritis: The Effects of Synovitis

Scanzello

2012

HSS Journal®: The Musculoskeletal Journal of Hospital for Speci

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The synovitis of "non-inflammatory" orthopaedic arthropathies: a quantitative histological and immunohistochemical analysis

Abstract: Synovial membranes from "non-inflammatory" arthropathies featured neovascularisation and inflammation intermediate between normal and OA synovium. These results expand previous findings that mechanical joint injury may lead to a mild-to-moderate synovitis.

Cited by 101 publications

References 11 publications

Functional mesenchymal stem cell niches in adult mouse knee joint synovium in vivo

Functional mesenchymal stem cell niches in adult mouse knee joint synovium in vivo

Does Intraarticular Inflammation Predict Biomechanical Cartilage Properties?

Pathologic and Pathogenic Processes in Osteoarthritis: The Effects of Synovitis

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