2010
DOI: 10.1002/jbm.a.32931
|View full text |Cite
|
Sign up to set email alerts
|

The synthesis and characterization of nanophase hydroxyapatite using a novel dispersant‐aided precipitation method

Abstract: The synthesis of nanophase hydroxyapatite (nHA) is of importance in the field of biomaterials and bone tissue engineering. The bioactive and osteoconductive properties of nHA are of much benefit to a wide range of biomedical applications such as producing bone tissue engineered constructs, coating medical implants, or as a carrier for plasmid DNA in gene delivery. This study aimed to develop a novel low‐temperature dispersant‐aided precipitation reaction to produce nHA particles (<100 nm), which are regarded a… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

4
79
0

Year Published

2012
2012
2020
2020

Publication Types

Select...
7
1

Relationship

4
4

Authors

Journals

citations
Cited by 94 publications
(83 citation statements)
references
References 41 publications
4
79
0
Order By: Relevance
“…The collagen nanohydroxyapatite (nHa) scaffold was fabricated by the addition 3.6g nHa to the standard collagen scaffold. nHa was synthesised using a novel dispersant-aided precipitation technique [48] which was incorporated into collagen slurries using a suspension method [40]. Scaffolds were cut to 9.5mm diameter using circular biopsy punches postlyophilisation.…”
Section: Scaffold Fabricationmentioning
confidence: 99%
“…The collagen nanohydroxyapatite (nHa) scaffold was fabricated by the addition 3.6g nHa to the standard collagen scaffold. nHa was synthesised using a novel dispersant-aided precipitation technique [48] which was incorporated into collagen slurries using a suspension method [40]. Scaffolds were cut to 9.5mm diameter using circular biopsy punches postlyophilisation.…”
Section: Scaffold Fabricationmentioning
confidence: 99%
“…[93] However, research carried out by our lab has optimized CaPs for this purpose, using a formulation of the hydroxyapatite mineral phase as nonaggregating nanoparticles (nHA) previously developed in-house. [94] These NPs were originally investigated to develop a collagen-nHA composite scaffold with improved mechanical properties; [94] this material was then adapted to surpass the pDNA transfection efficiency of a commercial CaP transfection kit in hMSCs, [95] and was further tailored to deliver both reporter miR-mimics and antagomiRs to the same cell type, with a single low dose maintaining a silencing efficiency of greater than 90% over 1 week. [96] Although little is known regarding the uptake mechanisms of CaP-nucleic acid complexes, their ability to form precipitates on the cell surface and undergo clathrin and dynamin-dependent endocytosis has been documented, [37] and their pH-dependent solubility allows complex dissociation to commence within intracellular compartments.…”
Section: Nonviral Vectorsmentioning
confidence: 99%
“…[96,124] Studies on scaffold-based gene delivery in the lab originally began with the delivery of pDNA including BMP2 [95] and combinations of BMP2 and VEGF [155] utilizing the nHA particles [94] and coll-nHA scaffolds [94,156] developed in-house specifically for bone repair. Having demonstrated the dramatically enhanced therapeutic potential of such systems not only for bone but also cartilage and nerve repair, we also progressed to investigating the utility of alternative gene delivery vectors including PEI, [85,155,157] chitosan, [40,158] Lipofectamine 2000, [75] and cyclodextrin, [75,159] different genetic cargo such as pDNA, [85,95,155,157] miRNA, [96,124] and siRNA, [75,159] and various scaffold types including collagen alone, [85,95] collagennHA, [75,95,96,124,155,159] collagen-HA [85] and collagen hyaluronic acid (coll-HyA) [85] to tailor our systems depending on the final application required.…”
Section: Osteogenesis and Bone Repairmentioning
confidence: 99%
“…15 Since then, a number of further enhanced, bone-targeted, novel collagen-composite scaffolds have been produced by our group including the collagen-nHa scaffold which will be discussed primarily in this commentary. 14,16,17 Previous work within our laboratory has demonstrated the ability 1990s. 36,37 The scaffold essentially acts as a depot for the gene while simultaneously offering structural support and a matrix for new tissue deposition (Fig.…”
mentioning
confidence: 93%