The synthesis and radiolabelling of novel markers of tissue hypoxia of the iodinated azomycin nucleoside class.

Schneider, Richard F.; Engelhardt, Edward L.; Stobbe, Corinne C.; Fenning, Matthew C.; Chapman, J. D.

doi:10.1002/(sici)1099-1344(199707)39:7<541::aid-jlcr5>3.0.co;2-b

Cited by 36 publications

(47 citation statements)

References 13 publications

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“…They were synthesized locally and radiolabelled using procedures that have been reported previously (Schneider et al, 1997). The kinetics of their covalent linkage to molecules within tumour cells in vitro and mouse tumours in vivo have also been reported (Chapman et al, 1996;Schneider et al, 1997).…”

Section: Materials and Methods Hypoxic Markersmentioning

confidence: 99%

“…Their selective survival, reoxygenation and eventual proliferation can produce local failure after treatment. Many factors associated with tumour cells and their interactions with host cells can modulate the oxygenation status of individual tumours as well as the number of clonogenic cells that are hypoxic and maximally radioresistant (Chapman, 1997).…”

mentioning

confidence: 99%

“…The distribution of HF values of individual tumours around their mean value can vary significantly and may be indicative of the intertumour heterogeneity of this specific property. Several different invasive and non-invasive assays of tumour oxygenation are under development for quantifying this tumour property before, during and after various therapies (Stone et al, 1993;Chapman, 1997). In that regard, bioreducible hypoxic markers have the potential for measuring the proportion of viable hypoxic cells within individual tumours (Chapman et al, 1981).…”

mentioning

confidence: 99%

“…Chronic hypoxia in tumours results from the proliferation of their cells to volumes beyond those that can be adequately supplied with oxygen delivered through tumour vasculature (Chapman, 1997). Such tumour growth can lead to cords and necrosis, which have been observed by conventional histology (Thomlinson and Gray, 1958).…”

mentioning

confidence: 99%

“…Two bioreducible hypoxic markers developed for the non-invasive detection of tumour hypoxia using nuclear medicine procedures (Schneider et al, 1997) were used to investigate spontaneous and induced oxygenation changes within individual rodent tumours. Differences in tumour avidity for these markers labelled with different radioisotopes of iodine and administered before and after oxygen modulation procedures were suggestive of changes in tumour HF.…”

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A dual hypoxic marker technique for measuring oxygenation change within individual tumors

Iyer

Kim²,

Schneider³

et al. 1998

Br J Cancer

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Summary Rodent tumour models have been the 'workhorse' for tumour oxygenation research and for investigating radiobiological hypoxic fraction. Because of the intertumour heterogeneity of blood flow and related parameters, most studies have pooled information derived from several different tumours to establish the statistical significance of specific measurements. But it is the oxygenation status of and its modulation in individual tumours that has important prognostic significance. In that regard, the bioreducible hypoxic marker technique was tested for its potential to quantify oxygenation changes within individual tumours. f-D-lodinated azomycin galactoside (IAZG) and P-Diodinated azomycin xylopyranoside (IAZXP) were each radiolabelled with lodine-125 and iodine-131 for measurements of animal tumour oxygenation. The tumour-blood (T/B) ratio of marker radioactivity in mice after the renal excretion of unbound marker (at 3 h and longer times) had been shown to be proportional to radiobiological hypoxic fraction. When markers labelled with both radioisotopes were administered simultaneously to EMT-6 tumour-bearing scid mice, T/B ratios were found to vary by up to 300% between different tumours, with an average intratumour variation of only -4%. When the markers were administered 2.5-3.0 h apart, changes in T/B ratios of 8-25% were observed in 10 out of 28 (36%) tumours. Changes to both higher and lower hypoxic fraction were observed, suggestive of acute or cycling hypoxia. When 0.8 mg g-1 nicotinamide plus carbogen was administered to increase tumour oxygenation, reductions in T/B ratios (mean AT/B -38%) were observed in all tumours. Similar results were obtained with Dunning rat prostate carcinomas growing in Fischer X Copenhagen rats whose T/B ratios of IAZG and radiobiological hypoxic fractions are significantly lower. These studies suggest that fluctuating hypoxia can account for at least 25% of the total hypoxic fraction in some tumours and that correlations between bioreducible marker avidity and related tumour properties will be optimal when the independent assays are performed over the same time period. This dual hypoxic marker technique should prove useful for investigating both spontaneous and induced oxygenation changes within individual rodent tumours.

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Section: Materials and Methods Hypoxic Markersmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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A dual hypoxic marker technique for measuring oxygenation change within individual tumors

Iyer

Kim²,

Schneider³

et al. 1998

Br J Cancer

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The Chemistry of Inorganic Nuclides (⁸⁶Y,⁶⁸Ga,⁶⁴Cu,⁸⁹Zr,¹²⁴I)

Price

Orvig

2014

The Chemistry of Molecular Imaging

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Carbohydrate‐Based Molecules for Molecular Imaging in Nuclear Medicine

Morais¹,

Falconer

Santos³

2013

Eur J Org Chem

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This review covers published literature describing the synthesis of labeled carbohydrates for use in molecular imaging, with a particular focus on the use of nuclear techniques (PET, SPECT). Recent advances in the radiosynthesis of [18F]FDG (electrophilic vs. nucleophilic radiofluorinations), a PET radiotracer based on glucose and the most widely PET tracer currently in use for cancer and inflammatory disease diagnosis, is considered. The powerful impact of [18F]FDG in the clinic has prompted intensive research efforts into glucose‐based radiotracers for PET and SPECT imaging. These achievements are also reviewed, along with the use of glycopeptides for nuclear molecular imaging. Finally, recent work on the radionuclide labeling of nucleosides and glycoconjugates is discussed.

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The synthesis and radiolabelling of novel markers of tissue hypoxia of the iodinated azomycin nucleoside class.

Cited by 36 publications

References 13 publications

A dual hypoxic marker technique for measuring oxygenation change within individual tumors

A dual hypoxic marker technique for measuring oxygenation change within individual tumors

The Chemistry of Inorganic Nuclides (⁸⁶Y,⁶⁸Ga,⁶⁴Cu,⁸⁹Zr,¹²⁴I)

Carbohydrate‐Based Molecules for Molecular Imaging in Nuclear Medicine

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The synthesis and radiolabelling of novel markers of tissue hypoxia of the iodinated azomycin nucleoside class.

Cited by 36 publications

References 13 publications

A dual hypoxic marker technique for measuring oxygenation change within individual tumors

A dual hypoxic marker technique for measuring oxygenation change within individual tumors

The Chemistry of Inorganic Nuclides (86Y,68Ga,64Cu,89Zr,124I)

Carbohydrate‐Based Molecules for Molecular Imaging in Nuclear Medicine

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The Chemistry of Inorganic Nuclides (⁸⁶Y,⁶⁸Ga,⁶⁴Cu,⁸⁹Zr,¹²⁴I)