The synthesis and use of thionphospholipids in 31P-NMR studies of lipid polymorphism

Lipidic intramembranous particles

“…This strongly suggests that in cardiolipin/ egg PC systems both lipids are present in the H~I tubes [33]. A similar conclusion has been obtained from 31P-NMR using thion-phospholipids [78]. The presence of PC in the cylinders could explain the increase in tube diameter, since this molecule is probably more hydrated and therefore has an overall cylindrical shape.…”

“…As has been hypothesized [46,78] and discussed above (subsection IVB. ), inverted micelles may be envisaged as intermediates during membrane fusion.…”

In Vitro Interaction between a Chloroplast Transit Peptide and Chloroplast Outer Envelope Lipids Is Sequence-specific and Lipid Class-dependent

“…In this strain, growth is tightly coupled to increased synthesis of CL, which in the presence of specific divalent cations (Mg 2ϩ ) will adopt a preference for the non-bilayer phase (53). These cells grow only in the presence of specific cations, indicating that polymorphic regulation of membrane-lipid composition is essential for cell viability.…”

A molecular model for the specific cardiolipin-presequence interactions

“…Moreover, the presence of phosphatidylethanolamine in the vesicles enhanced contact formation [ 101. Both mitochondria1 C L and phosphatidylethanolamine have a high tendency to form aggregate structures with concave interfaces [6,16], indicating that this property promotes contact formation. This could for instance be explained by a p25-induced semifusion intermediate.…”

“…Moreover, this lipid has a unique chemical structure in that it consists of two phosphatidyl moieties, which are linked by a glycerol bridge, and it therefore carries two negative charges and four acyl chains. The fatty-acid composition of mitochondrial CL is very unsaturated [5], which leads to a greater tendency to form structures with concave interfaces [6]. Presequences share no sequence similarity, but they are all positively charged and have the ability to form an amphiphilic a-helix.…”