The Synthesis of a 2D Ultra‐Large Protein Supramolecular Nanofilm by Chemoselective Thiol–Disulfide Exchange and its Emergent Functions

Xu, Yewei; Hu, Xinyi; Qin, Rongrong; Su, Hao; Li, Juling; Yang, Peng

doi:10.1002/ange.201912848

Cited by 7 publications

(6 citation statements)

References 70 publications

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“…Another reason for the initial fast release of the drug from the hydrogel is due to larger pore size of the hydrogel mesh than the size of disulfiram, so the release of drug could be further controlled in future experiments by using a crosslinker to enhance the cross-linking of the hydrogel network. 46 Constant release corresponds to the release of DSF from the compact hydrogel network because of enhanced cross-linking. These results are due to the osmotic pressure difference between the Ch/DSF hydrogel and the released medium.…”

Section: Resultsmentioning

confidence: 99%

“…The initial sudden DSF release from the hydrogel might be due to weak absorption of the drug in the hydrogel network or the quicker dispersion of DSF into the medium or both. Another reason for the initial fast release of the drug from the hydrogel is due to larger pore size of the hydrogel mesh than the size of disulfiram, so the release of drug could be further controlled in future experiments by using a crosslinker to enhance the cross-linking of the hydrogel network . Constant release corresponds to the release of DSF from the compact hydrogel network because of enhanced cross-linking.…”

Section: Resultsmentioning

confidence: 99%

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Thermosensitive Chitosan-Based Injectable Hydrogel as an Efficient Anticancer Drug Carrier

2020

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A thermosensitive, physically cross-linked injectable hydrogel was formulated for the effective and sustained delivery of disulfiram (DSF) to the cancer cells as there is no hydrogel formulation available until now for the delivery of DSF. As we know, hydrogels have an advantage over other drug delivery systems because of their unique properties, so we proposed to formulate an injectable hydrogel system for the sustained delivery of an anticancer drug (DSF) to cancer cells. To investigate the surface morphology, a scanning electron microscope study was carried out, and for thermal stability of hydrogels, TGA (thermogravimetric analysis) and DSC (differential scanning calorimetry) were performed. The rheological behavior of hydrogels was evaluated with the increasing temperature and time. These developed hydrogels possessing excellent biocompatibility could be injected at room temperature following rapid gel formation at body temperature. The swelling index and in vitro drug release studies were performed at different pH (6.8 and 7.4) and temperatures (25 and 37 °C). The cell viability of the blank hydrogel, free DSF solution, and Ch/DSF (chitosan/DSF)-loaded hydrogel was studied by MTT assay on SMMC-7721 cells for 24 and 48 h, which exhibited higher cytotoxicity in a dose-dependent manner in contrast to the free DSF solution. Moreover, the cellular uptake of DSF-loaded hydrogels was observed stronger as compared with free DSF. Hence, chitosan-based hydrogels loaded with DSF possessing exceptional properties can be used as a novel injectable anticancer drug for the sustained delivery of DSF for long-term cancer therapy.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Thermosensitive Chitosan-Based Injectable Hydrogel as an Efficient Anticancer Drug Carrier

2020

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“…Moving forward, our group reported the first example of amyloid-like lysozyme-cysteine nanofilm with an ultralarge area (900 cm 2 ) by a chemoselective exchange reaction between lysozyme and cysteine. 130 Inorganic CdTe QDs or Au NPs were encapsulated into the proteinaceous nanofilm with uniform distribution by simply mixing inorganic colloids, protein lysozyme, and cysteine in aqueous solution. The 2D inorganic NPs protein hybrid nanofilms offer great potential in many applications such as catalysis, biosensing, tissue engineering, and diagnostics.…”

Section: Amyloid-like Metal Hybrids (Almhs)mentioning

confidence: 99%

Metal–Protein Hybrid Materials with Desired Functions and Potential Applications

Saif

Yang

2021

ACS Appl. Bio Mater.

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Metal nanohybrids are fast emerging functional nanomaterials with advanced structures, intriguing physicochemical properties, and a broad range of important applications in current nanoscience research. Significant efforts have been devoted toward design and develop versatile metal nanohybrid systems. Among numerous biological components, diverse proteins offer avenues for making advanced multifunctional systems with unusual properties, desired functions, and potential applications. This review discusses the rational design, properties, and applications of metal–protein nanohybrid materials fabricated from proteins and inorganic components. The construction of functional biomimetic nanohybrid materials is first briefly introduced. The properties and functions of these hybrid materials are then discussed. After that, an overview of promising application of biomimetic metal–protein nanohybrid materials is provided. Finally, the key challenges and outlooks related to this fascinating research area are also outlined.

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“…Significantly, these hydrogels can be dissolved by reduction with low-molecular weight thiols and could therefore be used in controlled release applications. In a different approach, a protein-based nanofilm was developed by reducing the Cys6-Cys27 disulfide bond in lysozyme which could then oxidize and aggregate to form a proteinaceous film that encapsulated a range of molecules and particles [206].…”

Section: Biomaterialsmentioning

confidence: 99%

Can thiol-based redox systems be utilized as parts for synthetic biology applications?

Pillay

John

2021

Redox Report

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Objectives Synthetic biology has emerged from molecular biology and engineering approaches and aims to develop novel, biologically-inspired systems for industrial and basic research applications ranging from biocomputing to drug production. Surprisingly, redoxin (thioredoxin, glutaredoxin, peroxiredoxin) and other thiol-based redox systems have not been widely utilized in many of these synthetic biology applications. Methods We reviewed thiol-based redox systems and the development of synthetic biology applications that have used thiol-dependent parts. Results The development of circuits to facilitate cytoplasmic disulfide bonding, biocomputing and the treatment of intestinal bowel disease are amongst the applications that have used thiol-based parts. We propose that genetically encoded redox sensors, thiol-based biomaterials and intracellular hydrogen peroxide generators may also be valuable components for synthetic biology applications. Discussion Thiol-based systems play multiple roles in cellular redox metabolism, antioxidant defense and signaling and could therefore offer a vast and diverse portfolio of components, parts and devices for synthetic biology applications. However, factors limiting the adoption of redoxin systems for synthetic biology applications include the orthogonality of thiol-based components, limitations in the methods to characterize thiol-based systems and an incomplete understanding of the design principles of these systems.

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The Synthesis of a 2D Ultra‐Large Protein Supramolecular Nanofilm by Chemoselective Thiol–Disulfide Exchange and its Emergent Functions

Cited by 7 publications

References 70 publications

Thermosensitive Chitosan-Based Injectable Hydrogel as an Efficient Anticancer Drug Carrier

Thermosensitive Chitosan-Based Injectable Hydrogel as an Efficient Anticancer Drug Carrier

Metal–Protein Hybrid Materials with Desired Functions and Potential Applications

Can thiol-based redox systems be utilized as parts for synthetic biology applications?

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